Bacterial lipase and high-fat diets in canine exocrine pancreatic insufficiency: A new therapy of steatorrhea?

Suzuki, Ayako; Mizumoto, Akiyoshi; Sarr, M. G.; DiMagno, E.P.

doi:10.1053/gast.1997.v112.pm9178698

Cited by 68 publications

(34 citation statements)

References 4 publications

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“…Physiological studies have shown that preduodenal lipases are capable of acting not only in the stomach but also in the duodenum in synergy with a pancreatic lipase (14). Various clinical studies have been conducted on both animals and humans to assess the efficacy of enzymatic replacement therapies using acid-resistant lipases to treat exocrine pancreatic insufficiency (21). This treatment significantly increased the weight and reduced the steatorrhea in dogs.…”

mentioning

confidence: 99%

Crystal Structure of Human Gastric Lipase and Model of Lysosomal Acid Lipase, Two Lipolytic Enzymes of Medical Interest

Roussel

Canaan

Egloff

et al. 1999

Journal of Biological Chemistry

154

132

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Fat digestion in humans requires not only the classical pancreatic lipase but also gastric lipase, which is stable and active despite the highly acidic stomach environment. We report here the structure of recombinant human gastric lipase at 3.0-Å resolution, the first structure to be described within the mammalian acid lipase family. This globular enzyme (379 residues) consists of a core domain belonging to the ␣/␤ hydrolase-fold family and a "cap" domain, which is analogous to that present in serine carboxypeptidases. It possesses a classical catalytic triad (Ser-153, His-353, Asp-324) and an oxyanion hole (NH groups of Gln-154 and Leu-67). Four N-glycosylation sites were identified on the electron density maps. The catalytic serine is deeply buried under a segment consisting of 30 residues, which can be defined as a lid and belonging to the cap domain. The displacement of the lid is necessary for the substrates to have access to Ser-153. A phosphonate inhibitor was positioned in the active site that clearly suggests the location of the hydrophobic substrate binding site. The lysosomal acid lipase was modeled by homology, and possible explanations for some previously reported mutations leading to the cholesterol ester storage disease are given based on the present model.

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mentioning

confidence: 99%

Crystal Structure of Human Gastric Lipase and Model of Lysosomal Acid Lipase, Two Lipolytic Enzymes of Medical Interest

Roussel

Canaan

Egloff

et al. 1999

Journal of Biological Chemistry

154

132

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show abstract

“…This knowledge makes a considerable contribution to evaluation of intestinal and pancreatic functionality, especially in the case of pathologies such as malabsorption, maldigestion and steatorrhea, which are common in the dog [22].…”

Section: Discussionmentioning

confidence: 99%

Blood Lipids, Fecal Fat and Chymotrypsin Excretion in the Dog: Influence of Age, Body Weight and Sex

et al. 2004

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ABSTRACT. Effects of physiological variables (age, body weight and sex) on lipemia (total lipids, phospholipids, triglycerides and NEFA), fecal fat and chymotrypsin excretion were examined in dogs. On comparing various ages and body weights, they found statisticall y significant differences, while only total lipids showed a statistically significant difference between the two sexes. Moreover, there was a significant correlation between body weight and fecal fat and chymotrypsin excretion. The results obtained contribute to the evaluation of small intestine functionality, especially in relation to some diseases, as malabsorption, maldigestion and steatorrhea in the dog. KEY WORDS: blood lipid, canine, fecal chymotrypsin, fecal fat.

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“…Microsphere preparations have demonstrated a superior efficacy as compared to other preparations, and they facilitate fat digestion [42,43]. Pancreatic extracts constituted of fungal or bacterial lipases resistant to acid are now being developed; these preparations have already been tried successfully in steatorrhea therapy and they represent the future of substitutive therapy [44].…”

Section: Treatment Of Exocrine Pancreatic Insufficiency After Pancreamentioning

confidence: 99%

Pancreatic Exocrine Insufficiency Following Pancreatic Resection

Pezzilli¹

2012

Pancreatic Dis Ther

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Bacterial lipase and high-fat diets in canine exocrine pancreatic insufficiency: A new therapy of steatorrhea?

Cited by 68 publications

References 4 publications

Crystal Structure of Human Gastric Lipase and Model of Lysosomal Acid Lipase, Two Lipolytic Enzymes of Medical Interest

Crystal Structure of Human Gastric Lipase and Model of Lysosomal Acid Lipase, Two Lipolytic Enzymes of Medical Interest

Blood Lipids, Fecal Fat and Chymotrypsin Excretion in the Dog: Influence of Age, Body Weight and Sex

Pancreatic Exocrine Insufficiency Following Pancreatic Resection

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