Bacterial Lipoprotein-Based Vaccines Induce Tumor Necrosis Factor-Dependent Type 1 Protective Immunity against<i>Leishmania major</i>

Cote-Sierra, Javier; Bredan, Amin; Toldos, Carmen M.; Stijlemans, Benoı̂t; Brys, Lea; Cornélis, Pierre; Segovia, Manuel; Baetseĺier, Patrick De; Revets, Hilde

doi:10.1128/iai.70.1.240-248.2002

Cited by 35 publications

(31 citation statements)

References 43 publications

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“…Among these novel immunoreactive antigens, four lipoproteins (conserved hypothetical lipoprotein FTT1103, OmpA family protein, hypothetical membrane protein FTT1676 and lipoprotein FTT1591) and two signal peptidase I-cleaved proteins (hypothetical protein FTT1402, glycerophosphoryl diester phosphodiesterase) were predicted using LipoP program. The lipoproteins were successfully used as the potential protective antigens against some pathogens [22,23], thus they represent interesting targets for the vaccine development. The immunogenicity of OmpA protein has recently been reported in other bacteria [24,25].…”

Section: Discussionmentioning

confidence: 99%

Identification of immunoreactive antigens in membrane proteins enriched fraction from Francisella tularensis LVS

Janovská¹,

Pávková²,

Hubálek³

et al. 2007

Immunology Letters

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Section: Discussionmentioning

confidence: 99%

Identification of immunoreactive antigens in membrane proteins enriched fraction from Francisella tularensis LVS

Janovská¹,

Pávková²,

Hubálek³

et al. 2007

Immunology Letters

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“…The lipid moieties in lipoproteins are important in allowing the protein to assume its correct conformation. In addition, the lipid confers immunostimulatory and adjuvant properties to the protein and interacts with toll-like receptors (4,5,8,12,16,22). The pDUMP/ pCATCH plasmids are novel vectors engineered to express recombinant lipoproteins in E. coli (13).…”

Section: Discussionmentioning

confidence: 99%

Human Antibody Response to Outer Membrane Protein G1a, a Lipoprotein of Moraxella catarrhalis

et al. 2005

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Moraxella catarrhalis is an important cause of respiratory infections in adults with chronic obstructive pulmonary disease (COPD) and of otitis media in children. Outer membrane protein (OMP) G1a is an ϳ29-kDa surface lipoprotein and is a potential vaccine candidate. The gene that encodes OMP G1a was expressed and purified using a novel plasmid vector. [ 3 H]palmitic acid labeling demonstrated that both native and recombinant OMP G1a contain covalently bound palmitic acid. To assess the expression of OMP G1a during human infection, paired sera and sputum supernatants from adults with COPD followed prospectively were studied by enzyme-linked immunosorbent assays with recombinant lipidated OMP G1a to detect antibodies made specifically during carriage of M. catarrhalis. Overall, 23% of patients developed either a serum immunoglobulin G (IgG) response (9%) or sputum IgA response (21%) to OMP G1a, following 100 episodes of acquisition and clearance of M. catarrhalis. Patients developed antibody responses at similar rates following episodes of clinical exacerbation compared to asymptomatic colonization. Serum IgG antibodies following natural infection were directed predominantly at OMP G1a epitopes that are not exposed on the bacterial surface. These data show that OMP G1a is expressed during infection of the human respiratory tract and is a target of systemic and mucosal antibodies. These observations indicate that OMP G1a, a highly conserved surface protein, should be evaluated further as a vaccine candidate.Over the past 2 decades, Moraxella catarrhalis has emerged as an important human respiratory pathogen. Acquisition of a new strain of M. catarrhalis is associated with the development of an exacerbation in patients with chronic obstructive pulmonary disease (COPD) (43). Indeed, M. catarrhalis is the second most common bacterial cause of exacerbations in adults with COPD after nontypeable Haemophilus influenzae (32,38,44). COPD is the fourth-leading cause of death in the United States and is projected to rank fifth in the world by 2020 (2,26,28,39). M. catarrhalis is also the third-most-common bacterial cause of otitis media in children after Streptococcus pneumoniae and nontypeable H. influenzae (25). Approximately 25 million episodes of otitis media occur annually in the United States, and $2 billion is spent annually on treatment (25). It is estimated that M. catarrhalis causes 10% to 20% of cases of acute otitis media. Recurrent otitis media in infants and young children affects speech development and cognitive abilities (25, 42). Finally, M. catarrhalis is also a common cause of sinusitis in children and adults (32). Since M. catarrhalis is an important human pathogen for infants and adults with COPD, there is a need for a vaccine. Infants could be immunized to prevent otitis media and adults with COPD could be immunized to prevent exacerbations caused by M. catarrhalis.One approach to vaccine development for gram-negative bacteria has been to use outer membrane proteins (OMPs) as vaccine antigens. Effectiv...

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“…We found that OprI acts as a natural adjuvant that induces long-lived Th1 immune responses against heterologous Ags/peptides when fused to its Cterminal or admixed and provided protection upon subsequent challenge with the pathogen (16).…”

Section: Asthma Is An Inflammatory Lung Disease That Is Initiated Andmentioning

confidence: 99%

“…We have previously shown in a murine Leishmania model that OprI acts as a natural adjuvant that induces long-lasting Th1-type immune responses against heterologous Ags in vivo and provides protection against subsequent challenge with the given pathogen (16). Since bacterial lipoproteins are molecules that stimulate innate immune cells to produce proinflammatory cytokines, we analyzed the ability of lipoprotein OprI to activate and to mature immature DC.…”

Section: Opri Matures/activates Immature DC and Enhances Their Capacimentioning

confidence: 99%

Lipoprotein I, a TLR2/4 Ligand Modulates Th2-Driven Allergic Immune Responses

Revets

Pynaert

Grooten

et al. 2005

The Journal of Immunology

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Asthma is an inflammatory lung disease that is initiated and directed by Th2 and inhibited by Th1 cytokines. Microbial infections have been shown to prevent allergic responses by inducing the secretion of the Th1 cytokines IL-12 and IFN-γ. In this study, we examined whether administration of lipoprotein I (OprI) from Pseudomonas aeruginosa could prevent the inflammatory and physiological manifestations of asthma in a murine model of OVA-induced allergic asthma. OprI triggered dendritic cells to make IL-12 and TNF-α, with subsequent IFN-γ production from T cells. OprI stimulation of dendritic cells involved both TLR2 and TLR4. Intranasal coadministration of OprI with OVA allergen resulted in a significant decrease in airway eosinophilia and Th2 (IL-4 and IL-13) cytokines and this effect was sustained after repeated allergen challenge. The immediate suppressive effect of OprI (within 2 days of administration) was accompanied by an increase in Th1 cytokine IFN-γ production and a significant, but transient infiltration of neutrophils. OprI did not redirect the immune system toward a Th1 response since no increased activation of locally recruited Th1 cells could be observed upon repeated challenge with allergen. Our data show for the first time that a bacterial lipoprotein can modulate allergen-specific Th2 effector cells in an allergic response in vivo for a prolonged period via stimulation of the TLR2/4 signaling pathway.

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Bacterial Lipoprotein-Based Vaccines Induce Tumor Necrosis Factor-Dependent Type 1 Protective Immunity againstLeishmania major

Abstract: Immunity against

Cited by 35 publications

References 43 publications

Identification of immunoreactive antigens in membrane proteins enriched fraction from Francisella tularensis LVS

Identification of immunoreactive antigens in membrane proteins enriched fraction from Francisella tularensis LVS

Human Antibody Response to Outer Membrane Protein G1a, a Lipoprotein of Moraxella catarrhalis

Lipoprotein I, a TLR2/4 Ligand Modulates Th2-Driven Allergic Immune Responses

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