Bacterial Lux Biosensors in Genotoxicological Studies

Абилев, С. К.; Igonina, E. V.; Свиридова, Д. А.; Smirnova, S. V.

doi:10.3390/bios13050511

Cited by 3 publications

(1 citation statement)

References 59 publications

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“…In particular, E. coli MG1655 strains were used to express the lux operon from P. luminescens under the control of inducible promoter genes for evaluating both the oxidative and DNA-damaging activity of potential genotoxic compounds. The applicability of the use of these BL-based biosensors for rapid screening of antioxidant and radioprotective activities was confirmed by the analysis of 29 antioxidants and radioprotectors [ 75 ].…”

Section: Bl Biosensors Applied To Water Quality Monitoringmentioning

confidence: 99%

Illuminating Progress: The Contribution of Bioluminescence to Sustainable Development Goal 6—Clean Water and Sanitation—Of the United Nations 2030 Agenda

Gregucci,

Nazir,

Calabretta

et al. 2023

Sensors

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The United Nations Agenda 2030 Sustainable Development Goal 6 (SDG 6) aims at ensuring the availability and sustainable management of water and sanitation. The routine monitoring of water contaminants requires accurate and rapid analytical techniques. Laboratory analyses and conventional methods of field sampling still require considerable labor and time with highly trained personnel and transport to a central facility with sophisticated equipment, which renders routine monitoring cumbersome, time-consuming, and costly. Moreover, these methods do not provide information about the actual toxicity of water, which is crucial for characterizing complex samples, such as urban wastewater and stormwater runoff. The unique properties of bioluminescence (BL) offer innovative approaches for developing advanced tools and technologies for holistic water monitoring. BL biosensors offer a promising solution by combining the natural BL phenomenon with cutting-edge technologies. This review provides an overview of the recent advances and significant contributions of BL to SDG 6, focusing attention on the potential use of the BL-based sensing platforms for advancing water management practices, protecting ecosystems, and ensuring the well-being of communities.

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Section: Bl Biosensors Applied To Water Quality Monitoringmentioning

confidence: 99%

Illuminating Progress: The Contribution of Bioluminescence to Sustainable Development Goal 6—Clean Water and Sanitation—Of the United Nations 2030 Agenda

Gregucci,

Nazir,

Calabretta

et al. 2023

Sensors

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The ability of photochemical decomposition products of the Roundup to induce oxidative stress in bacterial cells

Saratovskikh,

Machigov,

Yarmolenko

et al. 2024

Ecological genetics

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BACKGROUND: The most common herbicide in agriculture for weed control is Roundup (Glyphosate), the active ingredient of which is N-phosphonomethylglycine (N-FMG). It is necessary to assess the hazard of the products of photochemical decomposition of N-FMG formed under the influence of solar UV and ozone. AIM: Using E. Coli lux-biosensors, a study of the ability of N-FMG photochemical degradation products to induce oxidative stress in bacterial cells. MATERIALS AND METHODS: Photochemical oxidation of an aqueous solution of N-FMG, mass spectrometry for the determination of N-FMG photochemical oxidation products and determination of their activity using E.coli (pSoxS-lux) and E.coli (pKatG-lux). RESULTS: Using biosensors, it was shown that the products of photochemical decomposition of N-PMG cause an increase in the concentration of superoxide anion radical and H2O2 in E. coli cells, which induces oxidative stress in the bacterial cell. CONCLUSION: The photochemical decomposition product of N-PMG induces the formation of superoxide anion radical and H2O2 in bacterial cells. Keywords: glyphosate (roundup, N-phosphonomethylglycine), photochemical degradation, mass spectrometry, E. coli, oxidative stress, biosensors pSoxS-lux, pKatG-lux.

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Analysis of cytogenetic disorders in residents of an industrial region in connection with work at coal-fired thermal power plants

Marushchak,

Minin

2024

Ecological genetics

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Background. In the air of an industrial environment associated with the processing and combustion of coal contains a huge amount of coal dust, heavy metals, polycyclic aromatic hydrocarbons, which have a negative impact on genetic stability. In this regard, the purpose of the study is to study the genotoxic effects in workers of coal thermal power plants. Materials and methods. The paper presents a cytogenetic analysis of genomic damage in 455 coal-fired thermal power plant workers compared with 533 control donors from Kemerovo using a micronucleus test. The formation of genomic abnormalities in coal-fired thermal power plant workers was assessed in relation to sex, age, smoking status, presence of chronic diseases, length of service, and working shops. Results. A significant increase in the frequency of occurrence of lymphocytes with micronuclei, nucleoplasmic bridges, nuclear buds, as well as cells at the stage of apoptosis in workers of coal-fired thermal power plants compared to the control group was established. An increase in the frequency of occurrence of cells with cytogenetic disorders was revealed in women working in coal production and workers over 51 years of age. Work experience and professional specialization had a significant impact on the formation of genomic disorders. Conclusion. The obtained results indicate a significant contribution of environmental factors to the development of geno- and cytotoxic effects in workers of coal-fired thermal power plants.

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Bacterial Lux Biosensors in Genotoxicological Studies

Cited by 3 publications

References 59 publications

Illuminating Progress: The Contribution of Bioluminescence to Sustainable Development Goal 6—Clean Water and Sanitation—Of the United Nations 2030 Agenda

Illuminating Progress: The Contribution of Bioluminescence to Sustainable Development Goal 6—Clean Water and Sanitation—Of the United Nations 2030 Agenda

The ability of photochemical decomposition products of the Roundup to induce oxidative stress in bacterial cells

Analysis of cytogenetic disorders in residents of an industrial region in connection with work at coal-fired thermal power plants

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