Bacterial meningitis pathogens identified in clinical samples using a SERS DNA detection assay

Gracie, Kirsten; Lindsay, Diane; Graham, Duncan; Faulds, Karen

doi:10.1039/c5ay00063g

Cited by 18 publications

(13 citation statements)

References 21 publications

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“…By contrast, label-based or extrinsic SERS combines optical activity of plasmonic materials (Ag, Au, Cu, etc.) functionalised with SERS-active messenger molecules (so-called Raman 'reporters'), which are resonant with a wide range of available excitation lasers [30]. The recognition element, e.g., antibody, enzyme, aptamer, etc., attached to NP surface binds to epitope(s) of specific target analytes (e.g., a metabolite, nucleic acid or bacterium) and its plasmonically enhanced characteristic SERS signal is measured indirectly through the Raman reporter.…”

Section: The Affinity Of Sers-active Substrates For Biochemical Molecmentioning

confidence: 99%

“…Here, SERS signals of target analytes are measured indirectly through SERS-active reporter molecules and recognition elements adsorbed onto NP surface. Notably, Gracie et al employed AgNPs labelled with a fluorescent reporter and biotin modification probes to detect multiple DNA sequences extracted from etiological agents of meningitis in clinical samples [30], extending on the picomolar quantitative detection of actual meningitis pathogens reported previously [31]. When utilised in this manner, SERS produces unique, sharp and well resolved analyte-specific signature peaks of exceptionally narrow bandwidth and fluorescence-free background.…”

Section: Introductionmentioning

confidence: 99%

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Enhancing Disease Diagnosis: Biomedical Applications of Surface-Enhanced Raman Scattering

et al. 2019

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Surface-enhanced Raman scattering (SERS) has recently gained increasing attention for the detection of trace quantities of biomolecules due to its excellent molecular specificity, ultrasensitivity, and quantitative multiplex ability. Specific single or multiple biomarkers in complex biological environments generate strong and distinct SERS spectral signals when they are in the vicinity of optically active nanoparticles (NPs). When multivariate chemometrics are applied to decipher underlying biomarker patterns, SERS provides qualitative and quantitative information on the inherent biochemical composition and properties that may be indicative of healthy or diseased states. Moreover, SERS allows for differentiation among many closely-related causative agents of diseases exhibiting similar symptoms to guide early prescription of appropriate, targeted and individualised therapeutics. This review provides an overview of recent progress made by the application of SERS in the diagnosis of cancers, microbial and respiratory infections. It is envisaged that recent technology development will help realise full benefits of SERS to gain deeper insights into the pathological pathways for various diseases at the molecular level.

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Section: The Affinity Of Sers-active Substrates For Biochemical Molecmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhancing Disease Diagnosis: Biomedical Applications of Surface-Enhanced Raman Scattering

et al. 2019

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“…Nam and co‐workers presented DNA‐based nanogap structures with quantitative and reproducible SERS signals . Graham and co‐workers reported Ag colloids for sensitive and reproducible detection of DNA . Our group also reported single‐crystalline noble metal NW‐based nanogap structures providing strong, reproducible, and stable SERS signals .…”

Section: Resultsmentioning

confidence: 63%

Nanogap‐Rich Au Nanowire SERS Sensor for Ultrasensitive Telomerase Activity Detection: Application to Gastric and Breast Cancer Tissues Diagnosis

Eom

Kim

Hwang

et al. 2017

Adv Funct Materials

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Telomerase has attracted much attention as a universal cancer biomarker because telomerase is overexpressed in more than 85% of human cancer cells while suppressed in normal somatic cells. Since a strong association exists between telomerase activity and human cancers, the development of effective telomerase activity assay is critically important. Here, a nanogaprich Au nanowire (NW) surface-enhanced Raman scattering (SERS) sensor is reported for detection of telomerase activity in various cancer cells and tissues. The nanogap-rich Au NWs are constructed by deposition of nanoparticles on single-crystalline Au NWs and provided highly reproducible SERS spectra. The telomeric substrate (TS) primer-attached nanogap-rich Au NWs can detect telomerase activity through SERS measurement after the elongation of TS primers, folding into G-quadruplex structures, and intercalation of methylene blue. This sensor enables us to detect telomerase activity from various cancer cell lines with a detection limit of 0.2 cancer cells mL −1 . Importantly, the nanogap-rich Au NW sensor can diagnose gastric and breast cancer tissues accurately. The nanogap-rich Au NW sensors show strong SERS signals only in the presence of tumor tissues excised from 16 tumorbearing mice, while negligible signals in the presence of heated tumor tissues or normal tissues. It is anticipated that nanogap-rich Au NW SERS sensors can be used for a universal cancer diagnosis and further biomedical applications including a diverse biomarker sensing.

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“…Most current applications of SERS for DNA analysis involve development of assays in which the structure of the target sequence is detected through hybridisation with a complimentary strand and it is the hydridisation event which is detected, typically by detecting the SERS signal from a label on the complimentary starnd. 3,4 This general approach is highly selective and sensitive and has been used to create clinically relevant assays. 5 Conversely, direct label-free analysis of DNA or RNA, although it has the advantage of simplicity, is not expected to rival hybridisation-based techniques for sequence determination because of its low positional sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Quantitative surface-enhanced Raman spectroscopy of single bases in oligodeoxynucleotides

Dick

Bell

2017

Faraday Discuss.

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To address the question of whether the SERS signals of ss-DNA are simply combinations of the signals from the individual bases that comprise the sequence, SERS spectra of unmodified ss-DNA sequences were obtained using a hydroxylamine-reduced Ag colloid aggregated with MgSO. Initially, synthetic oligodeoxynucleotides with systematic structural variations were used to investigate the effect of adding single nucleobases to the 3' terminus of 10-mer and 20-mer sequences. It was found that the resulting SERS difference spectra could be used to identify the added nucleobases since they closely matched reference spectra of the same nucleobase. Investigation of the variation in intensity of an adenine probe which was moved along a test sequence showed there was a small end effect where nucleobases near the 3' terminus gave slightly larger signals but the effect was minor (30%). More significantly, in a sample set comprising 25-mer sequences where A, T or G nucleobases were substituted either near the centres of the sequences or the 5' or 3' ends, the SERS difference spectra only matched the expected form in approximately half the cases tested. This variation appeared to be due to changes in secondary structure induced by altering the sequences since uncoiling the sequences in a thermal pre-treatment step gave difference spectra which in all cases matched the expected form. Multivariate analysis of the set of substitution data showed that 99% of the variance could be accounted for in a model with just three factors whose loadings matched the spectra of the A, T, and G nucleobases and which contained no positional information. This suggests that aside from the differences in secondary structure which can be eliminated by thermal pre-treatment, the SERS spectra of the 25-mers studied here are simply the sum of their component parts. Although this means that SERS provides very little information on the primary sequence it should be excellent for the detection of post-transcription modifications to DNA which can occur at multiple positions along a given sequence.

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Bacterial meningitis pathogens identified in clinical samples using a SERS DNA detection assay

Cited by 18 publications

References 21 publications

Enhancing Disease Diagnosis: Biomedical Applications of Surface-Enhanced Raman Scattering

Enhancing Disease Diagnosis: Biomedical Applications of Surface-Enhanced Raman Scattering

Nanogap‐Rich Au Nanowire SERS Sensor for Ultrasensitive Telomerase Activity Detection: Application to Gastric and Breast Cancer Tissues Diagnosis

Quantitative surface-enhanced Raman spectroscopy of single bases in oligodeoxynucleotides

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