Bacterial Metabolism and Transport Genes Are Associated with the Preference of Drosophila melanogaster for Dietary Yeast

Call, Tanner B.; Davis, Emma K.; Bean, Joseph D.; Lemmon, Skyler G.; Chaston, John M.

doi:10.1128/aem.00720-22

Cited by 2 publications

(1 citation statement)

References 61 publications

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“…The two fly lines were reported previously and were named F37 and ME89 and are called Fl and Me3 in this study (10). Adult female flies were briefly starved for 3 hours in clean, empty fly vials, and then introduced to the FlyPad arenas (54) as in our previous work (55). Each arena features two diet troughs that were prepared to contain 6.5 microliters of Y-G diet, except the preservative was omitted from the Y-G diet.…”

Section: Methodsmentioning

confidence: 99%

Humidity determines penetrance of a latitudinal gradient in genetic selection on the microbiota byDrosophila melanogaster

Massey,

Nosker,

Gale

et al. 2024

Preprint

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The fruit fly Drosophila melanogaster is a model for understanding how hosts and their microbial partners interact as the host adapts to wild environments. These interactions are readily interrogated because of the low taxonomic and numeric complexity of the flies' bacterial communities. Previous work has established that host genotype, the environment, diet, and interspecies microbial interactions can all influence host fitness and microbiota composition, but the specific processes and characters mediating these processes are incompletely understood. Here, we compared the variation in microbiota composition between wild-derived fly populations when flies could choose between the microorganisms in their diets and when flies were reared under environmental perturbation (different humidities). We also compared the colonization of the resident and transient microorganisms. We show that the ability to choose between microorganisms in the diet and the environmental condition of the flies can influence the relative abundance of the microbiota. There were also key differences in the abundances of the resident and transient microbiota. However, the microbiota only differed between populations when the flies were reared at humidities at or above 50% relative humidity. We also show that elevated humidity determined the penetrance of a gradient in host genetic selection on the microbiota that is associated with the latitude the flies were collected from. Finally, we show that the treatment-dependent variation in microbiota composition is associated with variation in host stress survival. Together, these findings emphasize that host genetic selection on the microbiota composition of a model animal host can be patterned with the source geography, and that such variation has the potential to influence their survival in the wild.

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Section: Methodsmentioning

confidence: 99%

Humidity determines penetrance of a latitudinal gradient in genetic selection on the microbiota byDrosophila melanogaster

Massey,

Nosker,

Gale

et al. 2024

Preprint

Self Cite

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Environment and diet shape the geography-specificDrosophila melanogastermicrobiota composition

Gale,

Kreutz,

Gottfredson Morgan

et al. 2024

Preprint

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Geographic and environmental variation in the animal microbiota can be directly linked to the evolution and wild fitness of their hosts but often appears to be disordered. Here, we sought to better understand patterns that underlie wild variation in the microbiota composition of Drosophila melanogaster. First, environmental temperature predicted geographic variation in fly microbial communities better than latitude did. The microbiota also differed between wild flies and their diets, supporting previous conclusions that the fly microbiota is not merely a reflection of diet. Flies feeding on different diets varied significantly in their microbiota composition, and flies sampled from individual apples were exceptionally depauperate for the Lactic Acid Bacteria (LAB), a major bacterial group in wild and laboratory flies. However, flies bore significantly more LAB when sampled from other fruits or compost piles. Follow-up analyses revealed that LAB abundance in the flies uniquely responds to fruit decomposition, whereas other microbiota members better indicate temporal seasonal progression. Finally, we show that diet-dependent variation in the fly microbiota is associated with phenotypic differentiation of fly lines collected in a single orchard. These last findings link covariation between the flies' dietary history, microbiota composition, and genetic variation across relatively small (single-orchard) landscapes, reinforcing the critical role that environment-dependent variation in microbiota composition can play in local adaptation and genomic differentiation of a model animal host.

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Bacterial Metabolism and Transport Genes Are Associated with the Preference of Drosophila melanogaster for Dietary Yeast

Cited by 2 publications

References 61 publications

Humidity determines penetrance of a latitudinal gradient in genetic selection on the microbiota byDrosophila melanogaster

Humidity determines penetrance of a latitudinal gradient in genetic selection on the microbiota byDrosophila melanogaster

Environment and diet shape the geography-specificDrosophila melanogastermicrobiota composition

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