Bacterial milking: A novel bioprocess for production of compatible solutes

Sauer, Thomas; Galinski, Erwin A.

doi:10.1002/(sici)1097-0290(19980705)59:1<128::aid-bit17>3.0.co;2-e

Cited by 67 publications

(23 citation statements)

References 26 publications

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“…Compatible solutes can be intracellularly stored at high concentrations without appreciable enzyme inhibition or inactivation (Brown et al 1986). The biotechnological potential of these compounds has also been shown to be due to their properties as stabilizers of biomolecules and whole cells (Frings et al 1995;Sauer and Galinski 1998).…”

Section: Introductionmentioning

confidence: 99%

Osmoadaptation in halophilic and alkaliphilic methanotrophs

Khmelenina

Kalyuzhnaya

Sakharovsky

et al. 1999

Archives of Microbiology

149

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By using (1)H- and (13)C-NMR spectroscopy, an accumulation of sucrose and two cyclic amino acids [ectoine (1,4,5, 6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid) and 5-oxoproline (pyrrolidone carboxylic acid)] was detected in the halotolerant methanotrophs Methylobacter alcaliphilus 20Z and Methylobacter modestohalophilus 10S. The organic solute pool was found to increase upon raising the NaCl concentration. In M. alcaliphilus 20Z, the intracellular level of the total solutes was shown to be sufficient to balance the osmotic pressure of the medium, whereas in M. modestohalophilus 10S their content was several times lower. Additionally, phosphatidylglycerol and phosphatidylcholine were predominant cell phospholipids in salt-adapted M. alcaliphilus 20Z. However, no phosphatidylcholine was found in M. modestohalophilus 10S, and the portion of phosphatidylglycerol increased while phosphatidylethanolamine decreased upon elevated external NaCl concentrations. Regularly arranged glycoprotein surface layers (S-layers) of hexagonal and linear (p2) symmetry were observed on the outer cell walls of M. alcaliphilus 20Z and M. modestohalophilus 10S. The S-layer in M alcaliphilus 20Z consisting of tightly packed, cup-shaped subunits was lost during growth at pH 7.2 (the lowest possible pH) in the absence of NaCl. Hence, osmoadaptation in the methanotrophs studied involves structure/function alterations of cell envelopes and changes in the chemical composition of membranes as well as de novo synthesis of compatible solutes.

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Section: Introductionmentioning

confidence: 99%

Osmoadaptation in halophilic and alkaliphilic methanotrophs

Khmelenina

Kalyuzhnaya

Sakharovsky

et al. 1999

Archives of Microbiology

149

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“…One of the osmolyes, ectoine, has been used to protect proteins and nucleic acids (Lippert and Galinski, 1992;Kolp et al, 2006). Ectoine is a common compatible solute in eubacteria and is produced by Halomonas elongata (Canovas et al, 1997;Sauer and Galinski, 1998). It should be mentioned that not only ectoine but also many other osmolytes confer similar protective effects on proteins and other macromolecules (Yancey et al, 1982;Arakawa and Timasheff, 1983, 1985Yancey, 2005).…”

Section: Industrial Applications Of Halophilic Organisms and Their Prmentioning

confidence: 99%

Halophilic Properties and their Manipulation and Application

et al. 2012

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“…This robustness of Halomonas elongate, together with the ability to achieve high cell densities, has led to its use in the manufacturing process for ectoines. The industrial bioprocess bacterial milking was designed for the large-scale production of ectoine by Sauer and Galinski (1998). In this process, Halomonas elongate is cultivated under high-salt conditions and ectoines are intracellularly accumulated.…”

Section: Production and Purification Of Extremolytesmentioning

confidence: 99%