Bacterial Nanocellulose as a Scaffold for In Vitro Cell Migration Assay

Ugrin, Milena; Dinić, Jelena; Jeremić, Sanja; Dragičević, Sandra; Djeri, Bojana Banovic; Nikolić, Aleksandra

doi:10.3390/nano11092322

Cited by 5 publications

(3 citation statements)

References 47 publications

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“…Due to the extraordinary threedimensional (3D) network structure of cellulose and its mechanical properties and expected biocompatibility, cellulose is an optimal material possibility for an assortment of tissue designing applications. A report showed the biocompatibility of BNC in cell culture (Ugrin et al, 2021). In the study, another mammalian cell culture substrate was created with an Acetobacter aceti delivered BNC.…”

Section: Nanocellulose In the Biomedical Fieldmentioning

confidence: 99%

Nanocellulose: Resources, Physio-Chemical Properties, Current Uses and Future Applications

Kaur¹,

Sharma²,

Munagala³

et al. 2021

Front. Nanotechnol.

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The growing environmental concerns due to the excessive use of non-renewable petroleum based products have raised interest for the sustainable synthesis of bio-based value added products and chemicals. Recently, nanocellulose has attracted wide attention because of its unique properties such as high surface area, tunable surface chemistry, excellent mechanical strength, biodegradability and renewable nature. It serves wide range of applications in paper making, biosensor, hydrogel and aerogel synthesis, water purification, biomedical industry and food industry. Variations in selection of source, processing technique and subsequent chemical modifications influence the size, morphology, and other characteristics of nanocellulose and ultimately their area of application. The current review is focused on extraction/synthesis of nanocellulose from different sources such as bacteria and lignocellulosic biomass, by using various production techniques ranging from traditional harsh chemicals to green methods. Further, the challenges in nanocellulose production, physio-chemical properties and applications are discussed with future opportunities. Finally, the sustainability of nanocellulose product as well as processes is reviewed by taking a systems view. The impact of chemicals, energy use, and waste generated can often negate the benefit of a bio-based product. These issues are evaluated and future research needs are identified.

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Section: Nanocellulose In the Biomedical Fieldmentioning

confidence: 99%

Nanocellulose: Resources, Physio-Chemical Properties, Current Uses and Future Applications

Kaur¹,

Sharma²,

Munagala³

et al. 2021

Front. Nanotechnol.

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“…Nanocellulose in the crystalline form has distinct effects on cell viability and function ( Mortensen et al, 2022 ) and can be complexed with proteins such as casein to form composites or coupled with lysine molecules to promote cell proliferation and differentiation ( Pandanaboina et al, 2021 ; Biranje et al, 2022 ). Much of the recent work with nanocellulose has relied on bacterial sources since it is low cost and easy to produce ( Ugrin et al, 2021 ), but the presence of endotoxin in such preparations, especially when culturing immune cells, remains a concern ( Moriyama et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Agarose/crystalline nanocellulose (CNC) composites promote bone marrow-derived mast cell integrity, degranulation and receptor expression but inhibit production of de novo synthesized mediators

Kulka

Wagner

Cho

et al. 2023

Front. Bioeng. Biotechnol.

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Introduction: Mast cells are highly granulated tissue-resident leukocytes that require a three-dimensional matrix to differentiate and mediate immune responses. However, almost all cultured mast cells rely on two-dimensional suspension or adherent cell culture systems, which do not adequately reflect the complex structure that these cells require for optimal function.Methods: Crystalline nanocellulose (CNC), consisting of rod-like crystals 4–15 nm in diameter and 0.2–1 µm in length, were dispersed in an agarose matrix (12.5% w/v), and bone marrow derived mouse mast cells (BMMC) were cultured on the agarose/CNC composite. BMMC were activated with the calcium ionophore A23187 or immunoglobulin E (IgE) and antigen (Ag) to crosslink high affinity IgE receptors (FcεRI).Results: BMMC cultured on a CNC/agarose matrix remained viable and metabolically active as measured by reduction of sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro) benzene-sulfonic acid hydrate (XTT), and the cells maintained their membrane integrity as analyzed by measuring the release of lactate dehydrogenase (LDH) and propidium iodide exclusion by flow cytometry. Culture on CNC/agarose matrix had no effect on BMMC degranulation in response to IgE/Ag or A23187. However, culture of BMMC on a CNC/agarose matrix inhibited A23187-and IgE/Ag-activated production of tumor necrosis factor (TNF) and other mediators such as IL-1β, IL-4, IL-6, IL-13, MCP-1/CCL2, MMP-9 and RANTES by as much as 95%. RNAseq analysis indicated that BMMC expressed a unique and balanced transcriptome when cultured on CNC/agarose.Discussion: These data demonstrate that culture of BMMCs on a CNC/agarose matrix promotes cell integrity, maintains expression of surface biomarkers such as FcεRI and KIT and preserves the ability of BMMC to release pre-stored mediators in response to IgE/Ag and A23187. However, culture of BMMC on CNC/agarose matrix inhibits BMMC production of de novo synthesized mediators, suggesting that CNC may be altering specific phenotypic characteristics of these cells that are associated with late phase inflammatory responses.

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“…However, these supports lack in the rigidity and elasticity when compared to the native in vivo tissues. Also, there is still no information about the culture of lung cells onto bacterial nanocellulose (BNC) matrices, a promising biomaterial for tissue engineering, whose physical‐mechanical characteristics provide a compatible 3D environment for in vitro approaches (Dos et al, 2018; Ugrin et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

CALU‐3 lung cells three‐dimensionally assembled onto CellFate® matrix present angiotensin‐converting enzyme‐2 activity

dos Santos,

dos Reis,

Berti

et al. 2023

Biotech & Bioengineering

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Currently, there is a great need for the development of three‐dimensional (3D) in vitro lung models. Particularly, the production of a suitable 3D model of pulmonary epithelium for understanding the pathophysiology of diseases such as the COVID‐19 must consider the tissue architecture and presence, for example, of the angiotensin‐converting enzyme‐2 (ACE‐2) in the cells. Different polymeric membranes are being used to support cell culturing, especially of lung cells, however, there is still no information about the culture of these cells onto bacterial nanocellulose (BNC) matrices. We have used the BNC matrix CellFate® as a support for the assembly of a 3D in vitro model of lung epithelium, composed of human lung fibroblasts (HLF) and lung adenocarcinoma cells (CALU‐3). CellFate® matrices were made from bacterial fermentation resulting in a natural and biocompatible biopolymer. Cells were cultured onto CellFate® and maintained in a 5% CO2 humidified atmosphere at 37°C. Cell viability was assessed by the resazurin method The samples were, then, exposed to the air–liquid interface (ALI), and histologically analyzed. ACE‐2 activity was verified on the hydrolyze of the fluorogenic substrate Mca‐APK(Dnp)‐OH, and its presence was evaluated by flow cytometry. The expression of the anionic transporter SLCO3A1 was evaluated by qPCR. Cell viability analysis indicates that CellFate® was not toxic to these cells. By flow cytometry, the presence of the ACE‐2 was identified in the CALU‐3 cells surface corroborating the results obtained from enzymatic activity analysis. The SLCO3A1 transporter expression was identified in cells cultured onto CellFate®, but not in cells cultured onto the transwell (control). CALU‐3 cells cultivated onto CellFate® resulted in a pseudostratified organization, a typical morphology of the human respiratory tract epithelium. The current model opens perspectives for studies involving physiological characterization, improving its relevance for the understanding of the pathophysiology of diseases as well as the response to drugs.

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Bacterial Nanocellulose as a Scaffold for In Vitro Cell Migration Assay

Cited by 5 publications

References 47 publications

Nanocellulose: Resources, Physio-Chemical Properties, Current Uses and Future Applications

Nanocellulose: Resources, Physio-Chemical Properties, Current Uses and Future Applications

Agarose/crystalline nanocellulose (CNC) composites promote bone marrow-derived mast cell integrity, degranulation and receptor expression but inhibit production of de novo synthesized mediators

CALU‐3 lung cells three‐dimensionally assembled onto CellFate® matrix present angiotensin‐converting enzyme‐2 activity

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