Bacterial Nanocellulose Fortified with Antimicrobial and Anti-Inflammatory Natural Products from Chelidonium majus Plant Cell Cultures

Abstract

In this work we developed a bi-functional Bacterial-Nano-Cellulose (BNC) carrier system for cell cultures of Chelidonium majus—a medicinal plant producing antimicrobial compounds. The porous BNC was biosynthesized for 3, 5 or 7 days by the non-pathogenic Komagataeibacter xylinus bacteria and used in three forms: (1) Without removal of K. xylinus cells, (2) partially cleaned up from the remaining K. xylinus cells using water washing and (3) fully purified with NaOH leaving no bacterial cells remains. The suspended C. majus cells were inoculated on the BNC pieces in liquid medium and the functionalized BNC was harvested and subjected to scanning electron microscopy observation and analyzed for the content of C. majus metabolites as well as to antimicrobial assays and tested for potential proinflammatory irritating activity in human neutrophils. The highest content and the most complex composition of pharmacologically active substances was found in 3-day-old, unpurified BNC, which was tested for its bioactivity. The assays based on the IL-1β, IL-8 and TNF-α secretion in an in vitro model showed an anti-inflammatory effect of this particular biomatrix. Moreover, 3-day-old-BNC displayed antimicrobial and antibiofilm activity against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. The results of the research indicated a possible application of such modified composites, against microbial pathogens, especially in local surface infections, where plant metabolite-enriched BNC may be used as the occlusive dressing.

Waste Rubber Incorporated in the Alkali-Activated Metakaolin’s Aluminosilicate Network Enhanced by Microwave Irradiation

“…However, one of the most sought-after characteristics of advanced biologically active materials is antimicrobial activity, which BC itself lacks. Therefore, BC is commonly modified with divergent antimicrobial agents, such as metal/metal oxide nanoparticles, antibiotics, and organic compounds (e.g., amino acids, curcumin, chitosan,…) (Zielińska et al 2022;Krasowski et al 2021;Moritz et al 2014).…”

Determination the optimal conditions for production of nanocellulose from acetic acid bacteria

“…2022) reported that HS broth pH (6.0), inoculated by 5% (v/v) from the strain Komagataeibacter maltaceti P285 then incubated at temperature 30°C for about 7 day, after cultivation, cellulose harvested then purified with high productivity. This medium was also used by Zieli´nska et al (2022) to produce high amounts of BC from another strain of AAB; K. xylinus. The nanocellulose termed as bacterial cellulose (BC) which produced through the bottom up, approach use the fermentation of low mwt.…”

Bio-elicitation stimulated isoquinoline alkaloids production in Chelidonium majus cells cultured on bio-nano-cellulose

“…C. majus cells were used as a model system for the production of protoberberine, protopine and benzophenanthridine derivatives. The plant cells were cultured on bacterial nanocellulose (BNC) carriers 1 . To enhance biosynthesis, three strains of microorganisms, i.e., Candida albicans, Staphylococcus aureus and Pseudomonas aeruginosa were inoculated at the top or the bottom of BNC discs.…”