Bacterial Nanocellulose Loaded with Bromelain: Assessment of Antimicrobial, Antioxidant and Physical-Chemical Properties

Ataide, Janaína Artem; Carvalho, Nathália Mendes de; Rebelo, Márcia de Araújo; Chaud, Marco V.; Grotto, Denise; Gerenutti, Marli; Rai, Mahendra; Mazzola, Priscila Gava; Jozala, Ângela Faustino

doi:10.1038/s41598-017-18271-4

Cited by 79 publications

(62 citation statements)

References 47 publications

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“…MIC method [23,29] was adapted was adapted in 96-well microplates and performed in triplicate. The plate was stored in oven at 37 º C overnight.…”

Section: Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

“…Immobilization of biomolecules in CB has also been studied, either by increasing its antibacterial or enzymatic, and may providing a control release system [23][24][25]. Although previous studies show the antimicrobial action on nisin incorporated in CB and its antioxidant capacity [26], it is necessary to investigate the stability of this system.…”

Section: Introductionmentioning

confidence: 99%

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Nisin-Loaded Bacterial Cellulose: Evaluation of Its Antimicrobial Activity Stability

Santos¹,

Soeiro²,

Ataide³

et al. 2020

Preprint

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Nisin is a 3.4 kDa antimicrobial peptide, produced by Lactococcus lactis (ATCC 11454). This bacteriocin can inhibit spores gemination and gram-positive bacteria development and has gained visibility in therapeutic use. The bacterial cellulose (CB) has been considered an ideal material and with high quality applied in food and medical-pharmaceutical inputs. Because of all this benefits, it is important to know the system proceeding of CB with nisin. Therefore, it was realize nisin release profile analysis of CBs was performed; analysis of the minimum inhibitory concentration (MIC) of nisin against Escherichia coli ATCC 25922, Pseudomonas aeuroginosa ATCC 9721 and Staphylococus aureus ATCC 10390; antimicrobial stability test, for 100 days at different temperatures of 4º, 25º and 37 ° C against microorganisms: S. aureus e L. sakei. The results show that nisin is released by the CB in 4 hours of contact with medium and the MIC of nisin is 78 µg/mL for S. aureus, doesn’t have gram-negative inhibition. It had stability until 100 days against L. sakei and 60 days for S. aureus. The system proved to be efficient and CB potentiated the antimicrobial action of nisin, acting as a selective barrier for other compounds present in the standard solution, serving as protection of the peptide at different temperatures. The CB loading system can be an ideal antimicrobial stability system for nisin.

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“…MIC method [23,29] was adapted was adapted in 96-well microplates and performed in triplicate. The plate was stored in oven at 37 º C overnight.…”

Section: Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nisin-Loaded Bacterial Cellulose: Evaluation of Its Antimicrobial Activity Stability

Santos¹,

Soeiro²,

Ataide³

et al. 2020

Preprint

Self Cite

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“…Local application of bromelain has been shown to be rapid, effective, non-invasive, safe, easily performed at the bedside with minimal or no blood loss and negligibly interfere with natural wound healing processes (Koller, Bukovcan, Orsag, Kvalteni, & Graffinger, 2008;Muhammad & Ahmad, 2017). Bromelain also accelerates the healing of burn wounds in humans (Krieger et al, 2012), showing antioxidant and antimicrobial activities (Ataide, de Carvalho, et al, 2017;Ali, Milala, & Gulani, 2015;Dutta & Bhattacharyya, 2013;Praveen et al, 2014;Manosroi, Chankhampan, Pattamapun, Manosroi, & Manosroi, 2014).…”

Section: Bromelainmentioning

confidence: 99%

“…Commercial enzymatic debriding agents are available, and some previous studies reported their efficacy mainly in the treatment of burn wounds (Cordts et al, 2016;Rosenberg et al, 2014;Schulz et al, 2017) and to clear bruises and hematomas on boxers (Blonstein, Bromelain (Dutta & Bhattacharyya, 2013;Ali et al, 2015;Praveen et al, 2014;Manosroi et al, 2014;Ataide, de Carvalho, et al, 2017;Ekambaram et al, 2017) (Dutta & Bhattacharyya, 2013;Hu et al, 2011;Singer et al, 2010;Wu et al, 2012) (Blonstein, 1960;Cordts et al, 2016;Koller et al, 2008;Krieger et al, 2012;Rosenberg et al, 2004;Rosenberg et al, 2014;Schulz et al, 2017) Propolis ( Centella asiatica (Hong et al, 2005;Shukla, Rasik, Jain, et al, 1999;Gohil et al, 2010;Incandela et al, 2001;Zhang et al, 2016) (Shukla, Rasik, Jain, et al, 1999;Sawatdee et al, 2016;Gohil et al, 2010;Gul Satar et al, 2013;Sunilkumar et al, 1998;Rosen et al, 1967;Incandela et al, 2001;Poizot & Dumez, 1978;Suguna et al, 1996;Zhang et al, 2016) …”

Section: Bromelainmentioning

confidence: 99%

Natural actives for wound healing: A review

Ataide

Cefali

Croisfelt

et al. 2018

Phytotherapy Research

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Nature has been a source of medicinal treatments for thousands of years, with the use of plants as prototypes for drug development and for the extraction of active compounds. Skin injuries occur regularly in everyday life, and the human skin has the ability to promote repair spontaneously under healthy conditions. However, some intrinsic and external factors may interfere with skins' natural ability, leading to nonhealing lesions and chronic wounds, which directly affect health and quality of life. Thus, attention should be given to this health problem, using an appropriated management when necessary. In this scenario, phytotherapy may be an option for cutaneous wound treatment, although further high-quality studies are needed to firmly establish the clinical efficacy of plants. This article reviews traditionally used natural actives for wound healing, highlighting their characteristics and mode of action.

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“…Other antimicrobial compounds are bromelain, a protease present in pineapple tissues, which also has anti-inflammatory and anticancer properties [243], lignin and lignin-derived compounds [244], and particularly curcumin, i.e. a naturally occurring polyphenolic compound isolated from Curcuma longa.…”

Section: Nanocellulose As a Carrier For Cell Delivery Into Skin Defectsmentioning

confidence: 99%

Nanocellulose in Biotechnology and Medicine: Focus on Skin Tissue Engineering and Wound Healing

Bacakova¹,

Pajorová²,

Bačáková³

et al. 2018

Preprint

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Nanocellulose is cellulose in the form of nanostructures, i.e. features not exceeding 100 nm at least in one dimension. These nanostructures include nanofibrils, e.g. in bacterial cellulose; nanofibers, e.g. in electrospun matrices; nanowhiskers and nanocrystals. These structures can be further assembled into bigger 2D and 3D nano-, micro- and macro-structures, such as nanoplatelets, membranes, films, microparticles and porous macroscopic matrices. There are four main sources of nanocellulose: bacteria (Gluonacetobacter), plants (trees, shrubs, herbs), algae (Cladophora) and animals (Tunicata). Nanocellulose has emerged for a wide range of industrial, technology and biomedical applications, e.g. for adsorption, ultrafiltration, packaging, conservation of historical artifacts, thermal insulation and fire retardation, energy extraction and storage, acoustics, sensorics, controlled drug delivery, and particularly for tissue engineering. Nanocellulose is promising for use in scaffolds for engineering of blood vessels, neural tissue, bone, cartilage, liver, adipose tissue, urethra and dura mater, for repairing connective tissue and congenital heart defects, and for constructing contact lenses and protective barriers. This review is focused on applications of nanocellulose in skin tissue engineering and wound healing as a scaffold for cell growth, for delivering cells into wounds, and as a material for advanced wound dressings coupled with drug delivery, transparency and sensorics. Potential cytotoxicity and immunogenicity of nanocellulose are also discussed.

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Bacterial Nanocellulose Loaded with Bromelain: Assessment of Antimicrobial, Antioxidant and Physical-Chemical Properties

Cited by 79 publications

References 47 publications

Nisin-Loaded Bacterial Cellulose: Evaluation of Its Antimicrobial Activity Stability

Nisin-Loaded Bacterial Cellulose: Evaluation of Its Antimicrobial Activity Stability

Natural actives for wound healing: A review

Nanocellulose in Biotechnology and Medicine: Focus on Skin Tissue Engineering and Wound Healing

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