Bacterial neuraminidase inhibitory linarin from Dendranthema zawadskii

Abstract

Dendranthema zawadskii is a one of the popular plants as native in South Korea. In this study, linarin was isolated and purified using silica-gel, Diaion, and Sephadex LH-20 from the aerial parts of D. zawadskii. The chemical structure was completely identified through spectroscopic data including 1D, 2D nucleic magnetic resonance, and HRFABMS. Furthermore, linarin inhibited the bacterial neuraminidase (BNA) activity with 13.5 μM of IC 50 dose-dependently. Through the enzyme kinetic experiments, linarin as BNA inhibitor exhibited a typical noncompetitive inhibition mode which K m was contestant and V max decreased as the concentration of the inhibitor increased. It was further identified that the inhibition constant was 16.0 μM. Linarin was the most abundance metabolite in the aerial part of D. zawadskii extract by UHPLC-TOF/MS analysis. Therefore, D. zawadskii and its main component are expected that it can be effectively used for the infection and inflammation caused by bacteria.

Inhibitory Potential of Quercetin Derivatives Isolated from the Aerial Parts of Siegesbeckia pubescens Makino against Bacterial Neuraminidase

“…They are responsible for the cleavage of glycol-conjugated proteins on the host cell membrane to release sialic acid [ 3 , 4 , 5 ]. In particular, bacterial neuraminidase (BNA) contributes to bacterial cell-to-cell signaling [ 6 ], which leads to the generation of self-extracellular polymeric substances (EPSs) that cause bacterium to adhere to one another, which is known as biofilms [ 7 , 8 ]. In addition, neuraminidase is also secreted by bacterial pathogens for the colonization of biofilms [ 9 ], which causes differences in the immune defense system by being present in bacterially infected sites of the human body [ 10 ].…”