Bacterial persistence

Liu, YunXiao; Yang, Jin; Zhao, Zhilun; Pu, Yonglin; Bai, Fan

doi:10.1007/s11426-014-5245-1

Cited by 4 publications

(6 citation statements)

References 63 publications

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“…Above its LCST, the hydrophobic surface of PNIPAAm promotes adsorption of extracellular matrix (ECM) with subsequent cell adhesion, while below the LCST, the high water content in the hydration layer prevents the adsorption of proteins, promoting the detachment of the cells. 37 This second explanation is relevant because in the previous sections (3.2 and 3.3), we have demonstrated that the grafting time influences the amount of hydrogel deposited, its morphology and its swelling properties. Therefore, the expanded and globule conformations are highly dependent on the grafting yield (GY).…”

Section: In Vitro Cell Culture Studiesmentioning

confidence: 75%

“…Bands observed in the range 3200-3600 cm À1 (labelled as 1 and 2) are attributed to -OH groups and to the stretching vibration of-NH 2 , respectively. 37 Secondly, the spectra of the grafted samples show the characteristic absorption bands at about 2800-3000 cm À1 , which are attributed to the stretching vibration of -CH 2 groups in the iPP backbone, covered by the band of -CH stretching of PNIPAAm at 2970 cm À1 (labelled as 3). 38 Finally, in all cases, the grafted samples exhibit characteristic absorption bands of PNIPAAm with sharp and strong intensities at wavenumbers of 1640 cm À1 (4, CQO stretching of amide I) and 1540 cm À1 (5, N-H bending of amide II).…”

Section: Effect Of the Crosslinker Concentration On The Composition Amentioning

confidence: 99%

“…Two possible explanations for such adhesion-and de-adhesion mechanisms have been reported in the literature. [28][29][30]37,41 It is well-known that ether groups are effective at repelling cells and proteins 42 and, as shown above by XPS ( Fig. 5), new C-O-C groups appeared as a result of the bond created by the grafting reaction between the hydroxyl groups on the plasma treated mesh surface and the NIPAAm monomer.…”

Section: In Vitro Cell Culture Studiesmentioning

confidence: 99%

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Polypropylene mesh for hernia repair with controllable cell adhesion/de-adhesion properties

et al. 2020

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Herein, a versatile bilayer system, composed by a polypropylene (PP) mesh and a covalently bonded poly(N-isopropylacrylamide) (PNIPAAm) hydrogel, is reported. The cell adhesion mechanism was successfully modulated by controlling the architecture of the hydrogel in terms of duration of PNIPAAm grafting time, crosslinker content, and temperature of material exposure in PBS solutions (below and above the LCST of PNIPAAm). The best in vitro results with fibroblast (COS-1) and epithelial (MCF-7) cells was obtained with a mesh modified with a porous iPP-g-PNIPAAm bilayer system, prepared via PNIPAAm grafting for 2 h at the lowest N,N 0 -methylene bis(acrylamide) (MBA) concentration (1 mM). Under these conditions, the detachment of the fibroblast-like cells was 50% lower than that of the control, after 7 days of cell incubation, which represents a high de-adhesion of cells in a short period. Moreover, the whole system showed excellent stability in dry or wet media, proving that the thermosensitive hydrogel was well adhered to the polymer surface, after PP fibre activation by cold plasma. This study provides new insights on the development of anti-adherent meshes for abdominal hernia repair.

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Section: In Vitro Cell Culture Studiesmentioning

confidence: 75%

Section: Effect Of the Crosslinker Concentration On The Composition Amentioning

confidence: 99%

Section: In Vitro Cell Culture Studiesmentioning

confidence: 99%

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Polypropylene mesh for hernia repair with controllable cell adhesion/de-adhesion properties

et al. 2020

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“…Persister is a bacteria subpopulation that grows slowly or in a dormant manner. The state of persistence can be induced by a variety of mechanisms, and their antibiotic susceptibility is generally reduced due to dormancy [ 3 ]. This feature of persisters makes them unable to be completely eliminated during antibiotic treatment, which increases the likelihood of resistance and can lead to infection recurrence [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Bacterial Persistence Mechanisms

Pan,

Liu,

et al. 2023

IJMS

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The recurrence of bacterial infectious diseases is closely associated with bacterial persisters. This subpopulation of bacteria can escape antibiotic treatment by entering a metabolic status of low activity through various mechanisms, for example, biofilm, toxin–antitoxin modules, the stringent response, and the SOS response. Correspondingly, multiple new treatments are being developed. However, due to their spontaneous low abundance in populations and the lack of research on in vivo interactions between persisters and the host’s immune system, microfluidics, high-throughput sequencing, and microscopy techniques are combined innovatively to explore the mechanisms of persister formation and maintenance at the single-cell level. Here, we outline the main mechanisms of persister formation, and describe the cutting-edge technology for further research. Despite the significant progress regarding study techniques, some challenges remain to be tackled.

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“…The overmining of soil bacteria, repeated discovery of similar scaffolds, and poor penetration of compounds into bacterial cells reduced the rate of antibiotic discovery 20–22 . Moreover, the discovery of antibiotics is facing potential emergence of bacterial resistance at any time 3,23 . In addition, the profit return on the investment of antibiotic development is difficult to estimate, which leads to a low innovation in the pharmaceutical industry.…”

Section: Introductionmentioning

confidence: 99%

Discovery, synthesis, and optimization of teixobactin, a novel antibiotic without detectable bacterial resistance

Tang

Wang

et al. 2022

Journal of Peptide Science

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Discovering new antibiotics with novel chemical scaffolds and antibacterial mechanisms presents a challenge for medicinal scientists worldwide as the ever‐increasing bacterial resistance poses a serious threat to human health. A new cyclic peptide‐based antibiotic termed teixobactin was discovered from a screen of uncultured soil bacteria through iChip technology in 2015. Teixobactin exhibits excellent antibacterial activity against all the tested gram‐positive pathogens and Mycobacterium tuberculosis, including drug‐resistant strains. Given that teixobactin targets the highly conserved lipid II and lipid III, which induces the simultaneous inhibition of both peptidoglycan and teichoic acid synthesis, the emergence of resistance is considered to be rather difficult. The novel structure, potent antibacterial activity, and highly conservative targets make teixobactin a promising lead compound for further antibiotic development. This review provides a comprehensive treatise on the advances of teixobactin in the areas of discovery processes, antibacterial activity, mechanisms of action, chemical synthesis, and structural optimizations. The synthetic methods for the key building block l‐allo‐End, natural teixobactin, representative teixobactin analogs, as well as the structure–activity relationship studies will be highlighted and discussed in details. Finally, some insights into new trends for the generation of novel teixobactin analogs and tips for future work and directions will be commented.

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Bacterial persistence

Cited by 4 publications

References 63 publications

Polypropylene mesh for hernia repair with controllable cell adhesion/de-adhesion properties

Polypropylene mesh for hernia repair with controllable cell adhesion/de-adhesion properties

Recent Advances in Bacterial Persistence Mechanisms

Discovery, synthesis, and optimization of teixobactin, a novel antibiotic without detectable bacterial resistance

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