Bacterial Populations Active in Metabolism of C ₁ Compounds in the Sediment of Lake Washington, a Freshwater Lake

Phylogenetic Study of Methanol Oxidizers from Chilika-Lake Sediments Using Genomic and Metagenomic Approaches

“…CS3, reportedly a novel strain from a brackish water environment, showed a lower similarity (85 %) with Methyloversatilis universalis (Table 3). Some reported gene sequences here matched with the gene sequences of active methylotrophs from Lake Washington [5] and from the deep sea [9].…”

“…Methylotrophs are distributed in diverse environments from freshwater Lake [5], deep-sea sediments [9], hypersaline lake [24], chlorinated environments [41], plant phyllosphere [42] to hot water effluent [43], suggesting their ubiquity. Present study focused on the aerobic methylotrophs covering 1,100 km 2 of the Chilika Lake sediments through established molecular markers (such as the functional gene mxaF and phylogenetic 16S rRNA gene probes) which demonstrated the presence of phylogenetically diverse aerobic methylotrophs.…”

“…Chilika, slowly but steadily transforming into a lagoon, is an attractive and unparalleled fusion of marine, river, and estuarine habitat that supports unique assemblage of marine, brackish water and freshwater microbes [2], presents a challenge to physiologists and biochemists [3]. Biological effects of siltation and salinity, overall biodiversity loss due to the changes in phytoplankton communities, and degradation of lake ecosystem owing to the freshwater invasive species thereby declining the overall productivity, have been profoundly documented in recent times [2,3,5,7,8].…”

“…Due to their specific properties, for instance, haloalkaliphilic methanotrophs could be of use in modern biotechnology [4]. Methylotrophs have been reported from diverse aquatic niches, such as, Washington Lake, USA [5], Mono Lake, USA [6], Lonar Lake, India [7], Weyerhaeuser [8], Warm pool, China [9], Colne Estuary, UK [10], Brackish marsh, Portugal [11], and as a marine symbiont [12]. There is increasing evidence for the striving methanotrophs in various ecogeographical regions including the saline and alkaline aquatic environments.…”

Active methylotrophs in the sediments of Lonar Lake, a saline and alkaline ecosystem formed by meteor impact

“…DNA-SIP successfully identified active methanotrophs and methylotrophs in Transbaikal soda lake sediments (Lin et al, 2004), Washington fresh water lake sediments (Nercessian et al, 2005), Colne estuary sediments (Moussard et al, 2009) and alkaline soils (pH 9) from a Chinese coal mine (Han et al, 2009). To our knowledge, DNA-SIP experiments have not been used to characterize methylotrophs utilizing methanol and methylamine in soda lakes.…”