Bacterial remediation of pesticide polluted soils: Exploring the feasibility of site restoration

Current Trends in Bioaugmentation Tools for Bioremediation: A Critical Review of Advances and Knowledge Gaps

“…Varner et al [ 53 ] explored the effect of the ecological growth strategies of plasmid donors and recipients on the conjugation and naphthalene degradation of two PAH-degrading plasmids, pNL1 and NAH7 [ 53 ]. Bokade et al [ 3 ] reviewed the suggested mobile genetic elements mediating the horizontal transfer of pesticide degradation genes, i.e., plasmids, transposons, genomic islands, transcription sequences, and integron gene cassettes.…”

“…Contaminated sites can act as a repository of highly adapted diverse populations which must be harnessed to score different degraders. Bokade et al [ 3 ] reviewed different enrichment approaches employed in the isolation of microorganisms from diverse environments, i.e., magnetic separation, differential centrifugation, micromanipulation, dilution to extinction, concentration to extinction, toxicity to extinction, heat pretreatment, and dilution-to-stimulation/extinction. Enrichment techniques offer an effective strategy for selectively isolating the microorganisms of interest.…”

“…Usually, this method involves the use of specific growth media and conditions that favour the growth of a specific microorganism over others. Several techniques including micromanipulation, magnetic separation, differential centrifugation, dilution-to-extinction, concentration-to-extinction, and toxicity-to-extinction have been applied for the enrichment and isolation of specific degrader microorganisms, depending on the intended purpose [ 3 ].…”

“…To select an optimal bioaugmentation strategy, further studies on the interactions of different functional bacteria related to their resistance to multiple stress factors, enzyme activity and system robustness are needed [ 2 ]. Rigorous research and critical analyses of available databases, as well as the incorporation of genetic engineering, nanotechnology, and systems biology can bring bioremediation to a more advanced level [ 3 ].…”

“…Bioaugmentation is a site-specific approach. Thus, recent research publications and reviews on bioaugmentation have focused on the following aspects: thermophilic reductive dechlorination [ 4 , 5 ]; the psychrophilic treatment of groundwaters [ 6 ]; microbially induced calcium carbonate precipitation techniques to mitigate the wind-induced erosion of calcareous desert sand [ 7 ]; hydrocarbon biodegradation in freshwater sediments from historically contaminated lakes [ 8 ]; the bacterial remediation of pesticide-polluted soils [ 3 ], emerging trends in the remediation of organic contaminated soils as a whole [ 9 ]; mechanisms of microbial activity in heavy metal removal [ 10 ]; comparisons of autochthonous and allochthonous bioaugmentation [ 11 ]; the stimulation of plant growth in bioaugmented hydroponic systems [ 12 ]; and the bioaugmentation of wastewaters (WWs) with yeast in the presence of antimicrobials [ 13 ], among others.…”