Bacterial Response to Oxidative Stress and RNA Oxidation

Seixas, André F.; Quendera, Ana Patrícia; Sousa, João Pedro Abreu; Silva, Alda F. Q.; Arraiano, Cecília M.; Andrade, José M.

doi:10.3389/fgene.2021.821535

Cited by 84 publications

(54 citation statements)

References 155 publications

(193 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…POD could scavenge excess free radicals, and the reduction in its activity would promote the oxidative damage of the bacteria, thereby hindering the growth and metabolism [ 43 ]. Figure 6 showed the results of bacterial POD activity after treatment with different concentrations of CEO.…”

Section: Results and Analysismentioning

confidence: 99%

Effects of Cinnamon Essential Oil on Oxidative Damage and Outer Membrane Protein Genes of Salmonella enteritidis Cells

Zhang

Zhao

Chen

et al. 2022

Foods

View full text Add to dashboard Cite

Salmonella is an important pathogen causing food poisoning. Food safety and health are the themes of today′s society. As a class of food-borne pathogens, Salmonella enteritidis had become one of the common zoonotic pathogens. Cinnamon essential oil (CEO) had been reported as an antibacterial agent, but there are few studies on its antibacterial mechanism. This study investigated the effects of CEO on oxidative damage and outer membrane protein genes of Salmonella enteritidis cells. First, the reactive oxygen species content in bacteria treated with different concentrations of cinnamon essential oil was determined by fluorescence spectrophotometry, and the effects of superoxide dismutase (SOD), catalase (CAT) and superoxide dismutase (SOD), and catalase (CAT) and peroxidase (POD) were determined by the kit method. The activity of POD and the content of malondialdehyde (MDA) were investigated to investigate the oxidative damage of CEO to Salmonella enteritidis cells. By analyzing the effect of CEO on the Salmonella enteritidis cell membrane’s outer membrane protein gene expression, the mechanism of CEO′s action on the Salmonella enteritidis cell membrane was preliminarily discussed. The results showed that CEO treatment had an obvious oxidative damaging effect on Salmonella enteritidis. Compared with the control group, the increase in CEO concentration caused a significant increase in the bacteria ROS content. The observation technique experiment found that with the increase in CEO concentration, the number of stained cells increased, which indicated that CEO treatment would increase the ROS level in the cells, and it would also increase with the increase in CEO concentration, thus causing the oxidation of cells and damage. In addition, CEO treatment also caused the disruption of the balance of the cellular antioxidant enzymes (SOD, CAT, POD) system, resulting in an increase in the content of MDA, a membrane lipid metabolite, and increased protein carbonylation, which ultimately inhibited the growth of Salmonella enteritidis. The measurement results of cell membrane protein gene expression levels showed that the Omp genes to be detected in Salmonella enteritidis were all positive, which indicated that Salmonella enteritidis carried these four genes. Compared with the control group, the relative expressions of OmpF, OmpA and OmpX in the CEO treatment group were significantly increased (p < 0.05), which proved that the cell function was disturbed. Therefore, the toxicity of CEO to Salmonella enteritidis could be attributed to the damage of the cell membrane and the induction of oxidative stress at the same time. It was speculated that the antibacterial mechanism of CEO was the result of multiple effects. This work was expected to provide a theoretical basis for the development of new natural food preservatives and the prevention and control of Salmonella enteritidis.

show abstract

Section: Results and Analysismentioning

confidence: 99%

Effects of Cinnamon Essential Oil on Oxidative Damage and Outer Membrane Protein Genes of Salmonella enteritidis Cells

Zhang

Zhao

Chen

et al. 2022

Foods

View full text Add to dashboard Cite

show abstract

“…Antibiotic treatment can perturb bacterial metabolism and increase production of reactive metabolic byproducts (RMB), such as reactive oxygen species (ROS). These reactive metabolites are well known for their ability to damage macromolecules, such as DNA and proteins, and they can be mutagenic (Markkanen, 2017;Seixas et al, 2022;Wong et al, 2022). Although even sub-lethal doses of antibiotics are sufficient to increase mutagenesis that leads to increased minimum inhibitory concentrations, scavenging these reactive metabolites could prevent this effect (Kohanski et al, 2007;Kohanski et al, 2010).…”

Section: Dampening Mutagenic Stressorsmentioning

confidence: 99%

Resistance-resistant antibacterial treatment strategies

Batchelder¹,

Hare²,

Mok³

2023

Front. Antibiot.

View full text Add to dashboard Cite

Antibiotic resistance is a major danger to public health that threatens to claim the lives of millions of people per year within the next few decades. Years of necessary administration and excessive application of antibiotics have selected for strains that are resistant to many of our currently available treatments. Due to the high costs and difficulty of developing new antibiotics, the emergence of resistant bacteria is outpacing the introduction of new drugs to fight them. To overcome this problem, many researchers are focusing on developing antibacterial therapeutic strategies that are “resistance-resistant”—regimens that slow or stall resistance development in the targeted pathogens. In this mini review, we outline major examples of novel resistance-resistant therapeutic strategies. We discuss the use of compounds that reduce mutagenesis and thereby decrease the likelihood of resistance emergence. Then, we examine the effectiveness of antibiotic cycling and evolutionary steering, in which a bacterial population is forced by one antibiotic toward susceptibility to another antibiotic. We also consider combination therapies that aim to sabotage defensive mechanisms and eliminate potentially resistant pathogens by combining two antibiotics or combining an antibiotic with other therapeutics, such as antibodies or phages. Finally, we highlight promising future directions in this field, including the potential of applying machine learning and personalized medicine to fight antibiotic resistance emergence and out-maneuver adaptive pathogens.

show abstract

“…The enrichment of the oxidoreductase activity GO term [GO:0016491] among the COVID-19 survived cohort included underlying genes such as quinone oxidoreductase, pyruvate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, and glyceraldehyde-3-phosphate dehydrogenase. Lung disease may become more severe in COVID-19 with increased oxidative stress, and it is possible that bacterial response in the COVID-19 survived cohort helped to reduce the oxidative stress [49][50][51] .…”

Section: Namementioning

confidence: 99%

Analysis of bronchoalveolar lavage fluid metatranscriptomes among patients with COVID-19 disease

Jochum

Lee

Curry

et al. 2022

Sci Rep

View full text Add to dashboard Cite

To better understand the potential relationship between COVID-19 disease and hologenome microbial community dynamics and functional profiles, we conducted a multivariate taxonomic and functional microbiome comparison of publicly available human bronchoalveolar lavage fluid (BALF) metatranscriptome samples amongst COVID-19 (n = 32), community acquired pneumonia (CAP) (n = 25), and uninfected samples (n = 29). We then performed a stratified analysis based on mortality amongst the COVID-19 cohort with known outcomes of deceased (n = 10) versus survived (n = 15). Our overarching hypothesis was that there are detectable and functionally significant relationships between BALF microbial metatranscriptomes and the severity of COVID-19 disease onset and progression. We observed 34 functionally discriminant gene ontology (GO) terms in COVID-19 disease compared to the CAP and uninfected cohorts, and 21 GO terms functionally discriminant to COVID-19 mortality (q < 0.05). GO terms enriched in the COVID-19 disease cohort included hydrolase activity, and significant GO terms under the parental terms of biological regulation, viral process, and interspecies interaction between organisms. Notable GO terms associated with COVID-19 mortality included nucleobase-containing compound biosynthetic process, organonitrogen compound catabolic process, pyrimidine-containing compound biosynthetic process, and DNA recombination, RNA binding, magnesium and zinc ion binding, oxidoreductase activity, and endopeptidase activity. A Dirichlet multinomial mixtures clustering analysis resulted in a best model fit using three distinct clusters that were significantly associated with COVID-19 disease and mortality. We additionally observed discriminant taxonomic differences associated with COVID-19 disease and mortality in the genus Sphingomonas, belonging to the Sphingomonadacae family, Variovorax, belonging to the Comamonadaceae family, and in the class Bacteroidia, belonging to the order Bacteroidales. To our knowledge, this is the first study to evaluate significant differences in taxonomic and functional signatures between BALF metatranscriptomes from COVID-19, CAP, and uninfected cohorts, as well as associating these taxa and microbial gene functions with COVID-19 mortality. Collectively, while this data does not speak to causality nor directionality of the association, it does demonstrate a significant relationship between the human microbiome and COVID-19. The results from this study have rendered testable hypotheses that warrant further investigation to better understand the causality and directionality of host–microbiome–pathogen interactions.

show abstract

Bacterial Response to Oxidative Stress and RNA Oxidation

Cited by 84 publications

References 155 publications

Effects of Cinnamon Essential Oil on Oxidative Damage and Outer Membrane Protein Genes of Salmonella enteritidis Cells

Effects of Cinnamon Essential Oil on Oxidative Damage and Outer Membrane Protein Genes of Salmonella enteritidis Cells

Resistance-resistant antibacterial treatment strategies

Analysis of bronchoalveolar lavage fluid metatranscriptomes among patients with COVID-19 disease

Contact Info

Product

Resources

About