Bacterial Ribosomes and Protein Synthesis

McQuillen, Kenneth

doi:10.1099/00221287-29-1-53

Cited by 2 publications

(1 citation statement)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…FloA-localizing regions were identified in the tomograms as 100- to 300-nm light electron-dense membrane areas that stained poorly with uranyl acetate and showed accumulation of several nanodomains rather than a single uniform microdomain. The cytoplasmic area surrounding these membrane regions concentrated electron-dense small particles, previously characterized as large protein complexes ( McQuillen, 1962 , Palade, 1955 ). We did not detect concentration of these particles in Δ floA tomograms ( Movie S2 ), which suggested that FMMs engage in active protein organization, metabolism, and/or trafficking.…”

Section: Resultsmentioning

confidence: 99%

Membrane Microdomain Disassembly Inhibits MRSA Antibiotic Resistance

García-Fernández

Koch

Rm³

et al. 2017

Cell

207

236

View full text Add to dashboard Cite

SummaryA number of bacterial cell processes are confined functional membrane microdomains (FMMs), structurally and functionally similar to lipid rafts of eukaryotic cells. How bacteria organize these intricate platforms and what their biological significance is remain important questions. Using the pathogen methicillin-resistant Staphylococcus aureus (MRSA), we show here that membrane-carotenoid interaction with the scaffold protein flotillin leads to FMM formation, which can be visualized using super-resolution array tomography. These membrane platforms accumulate multimeric protein complexes, for which flotillin facilitates efficient oligomerization. One of these proteins is PBP2a, responsible for penicillin resistance in MRSA. Flotillin mutants are defective in PBP2a oligomerization. Perturbation of FMM assembly using available drugs interferes with PBP2a oligomerization and disables MRSA penicillin resistance in vitro and in vivo, resulting in MRSA infections that are susceptible to penicillin treatment. Our study demonstrates that bacteria possess sophisticated cell organization programs and defines alternative therapies to fight multidrug-resistant pathogens using conventional antibiotics.

show abstract

Section: Resultsmentioning

confidence: 99%