Bacterial sensors based on biosilica immobilization for label-free OWLS detection

Adányi, Nóra; Bori, Zsuzsanna; Szendrő, István; Erdélyi, Katalin; Wang, Xiaohong; Schröder, Heinz C.; Müller, Wernér E.G.

doi:10.1016/j.nbt.2013.01.006

Cited by 13 publications

(7 citation statements)

References 26 publications

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“…Additionally, a proof-of-concept device has been developed using biosilica expressed in genetically modified E. coli for the attachment onto the sensor surface. The whole cell receptor was described for the detection of penicillin G (antibiotic), chloramphenicol (antibiotic), carbofuran (pesticide) and H2O2 [24]. Finally, OWLS technology has also been applied for the label-free detection of Salmonella typhimurium [25] and Legionella pneumophila [26] in water, using antibodies as biorecognition elements.…”

Section: Grating Biosensorsmentioning

confidence: 99%

Nanophotonic label-free biosensors for environmental monitoring

Chocarro-Ruiz

Gavela

Herranz

et al. 2017

Current Opinion in Biotechnology

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Section: Grating Biosensorsmentioning

confidence: 99%

Nanophotonic label-free biosensors for environmental monitoring

Chocarro-Ruiz

Gavela

Herranz

et al. 2017

Current Opinion in Biotechnology

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“…7, Fig. 8) [23], [24]. Different applications of nanotechnology are summarized, especially the application of evanescent wave based optical techniques and their role in food industry.…”

Section: Nanotechnology Based Microbial Sensorsmentioning

confidence: 99%

OWLS Based Nanosensors for Agro-Environmental and Food Safety

Adányi¹,

Szendrő²,

Székacs³

2017

JOAAT

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With globalized trade of food commodities, food safety and related environmental safety issues have become a central concern not only at national/regional levels, but all over the world. Consumers are concerned about food-borne pathogens and their toxic substances, including mycotoxins, as well as chemicals, including residues of pesticides and veterinary drugs. Environmental pollution may also lead to contamination of food/feed commodities grown in affected land. For the quick and reliable detection of hazardous substances, new types of sensors based on evanescent wave optical techniques on the rise to detect food-borne contaminants. Our results, obtained by Optical Waveguide Lightmode Spectroscopy (OWLS) based label-free immmunosensors used for determination of different chemical contaminants in food responsible for the risk of food poisoning, are presented. Measuring methods were investigated for the determination of herbicide active ingredient trifluralin and biomarker protein vitellogenin for monitoring the pollution with endocrine disrupting chemicals, as well as for different mycotoxins (aflatoxin, zearalenone, deoxynivalenol) and microbials. High specificity/selectivity of the sensitised surface coupled with high sensitivity of OWLS detection gives the possibility to develop immunosensors and microbial sensors in most cases with definitely lower limits of detection than those in traditionally used immunoassays in direct/competitive formats.  Index Terms-optical waveguide lightmode spectroscopy, nanobiosensor, agro-environmental safety, food safety I. EVANESCENT WAVE OPTICAL TECHNIQUES Nanotechnology has a multiple and rapidly expanding role in food industry. Nanomaterials are used in food production or inspection with their unique, nano-sized dispersity, or as novel methodologies utilizing Manuscript

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“…This technique allows in situ and label-free studies of surface processes at molecular level. The formation of a biosilica shell around E. coli transformed with the silicatein gene ) has been used to develop a bacterial biosensor by immobilizing the bacterial cells at mild conditions in the presence of TEOS on the surface of the SiO 2 -containing sensor chips (Adányi et al 2013b). The mass of the bound molecules or cells can be calculated from the change of the resonance angle.…”

Section: Nanosensorsmentioning

confidence: 99%

“…It is based on the measurement of the resonance angle of polarized laser light which is diffracted by a grating and incoupled into a thin waveguide layer (Ramsden 1999). This bacterial biosensor has been successfully used as an effective tool for the detection of various stressors (e.g., hydrogen peroxide), environmental pollutants (pesticides, e.g., carbofuran), and antibiotics (e.g., chloramphenicol) in real-time measurement (Adányi et al 2013b). The OWLS technique can also be applied for the detection and discrimination of living and damaged bacterial cells (Adányi et al 2006).…”

Section: Nanosensorsmentioning

confidence: 99%