Bacterial species-characteristic profiles of molecular species, and the antigenicity of phospholipids and glycolipids in symbiotic Lactobacillus, Staphylococcus and Streptococcus species

Iwamori, Masao; Nakasa, Masanori; Yamazaki, Kentaro; Iwamori, Yuriko; Tanaka, Kyoko; Aoki, Daisuke; Adachi, Souichi; Nomura, Taisei

doi:10.1007/s10719-012-9393-2

Cited by 7 publications

(4 citation statements)

References 24 publications

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“…Cholera toxin B-subunit was purchased from Sigma (S. Louis, MO, USA), and an antibody against cholera toxin Bsubunit was generated by immunization of a rabbit together with Freund's complete adjuvant (Sigma). Murine monoclonal anti-sulfatide (TCS-1) and anti-GM2 (Y916) antibodies were also generated by a conventional procedure in our laboratory [22]. Murine monoclonal anti-Le a plus Le b (MSN-1), anti-sialyl Le a (CA19-9), and anti-GM3 (M2590) antibodies were kindly donated by Prof. Nozawa S., Keio University, Tokyo, Dr. Matsumoto K., Mikuri Immun.…”

Section: Methodsmentioning

confidence: 99%

“…For structural analysis, individual gangliosides were purified from the acidic lipids using a silica gel (Iatrobeads 6RS8060: Iatron Laboratory, Tokyo) column by gradient elution with chloroform/methanol/water (55:45: 2 and 10:90:4, by vol.). The purified glycolipids were analyzed by negative ion FAB-MS (JMS-700TKM; JEOL, Tokyo) as described previously [22].…”

Section: Methodsmentioning

confidence: 99%

“…), which were then blocked with blocking buffer (PBS containing 1 % polyvinylpyrrolidone and 1 % ovalbumin), and the spots were visualized by immunostaining with the above anti-glycolipid antibodies diluted 1:500 to 1:2000 with dilution buffer (PBS containing 3 % polyvinylpyrrolidone), followed by immunostaining with peroxidase-conjugated anti-murine IgG, A and M antibodies (1:1000; Jackson Immunoresearch Lab., PA, USA), and peroxidase substrates, 4-chloro-1-naphthol and H 2 O 2 , according to the procedure reported previously [22]. Standard glycolipids (10 to 500 ng), GM3, GM2, GalNAc-GM1b and GalNAc-GD1a, were developed on the same TLC plates for the preparation of standard curves.…”

Section: Methodsmentioning

confidence: 99%

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Enhanced expression of unique gangliosides with GM2-determinant in human uterine cervical carcinoma-derived cell lines

et al. 2016

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Monoclonal antibody YHD-06 generated by immunization with GM2 reacted with gangliosides with GM2-determinant, i.e., GM2, GalNAc-GM1b and GalNAc-GD1a, among which GalNAc-GD1a was characterized as an antigen of autoimmune peripheral neuropathies including Guillain-Barré syndrome. When glycolipids were examined by TLC-immunostaining with YHD-06 in seven human cervical carcinoma-derived cell lines, GM2 was found in all cell lines, amounting to 15.5 % to 57.5 % of total gangliosides. Whereas GalNAc-GD1a was present in three cell lines, amounting to 5.4-17.5 % of total gangliosides, and GalNAc-GM1b in four cell lines in amounts of less than 2 %. The elevated amounts of gangliosides with GM2 determinant were closely correlated with the relative intensities of gene expression of GalNAc transferase, this being characteristic of cervical carcinoma-derived cells. However, in tissues from patients with several histological types of cervical carcinomas, GM3 was ubiquitously expressed in amounts of more than 66 % of total gangliosides, GM2 was expressed in only five of 15 tissues, and both GalNAc-GM1b and GalNAc-GD1a were not even detected in trace amounts. Since GM1 was detected in all tissues in amounts of less than 0.06 μg/mg dried tissue, all cervical carcinoma tissues were revealed to exhibit GM2 synthesis, indicating that enhanced synthesis of gangliosides with GM2 determinant is a characteristic of cultivated cells in vitro. Similarly, although I(3)SO3-GalCer was not present in the squamous cell carcinoma (SCC) tissues, SCC-derived cells selectively expressed II(3)SO3-LacCer. Since enhanced synthesis of GM2 has been reported in SV-40 virus-transfected fibroblasts, papilloma virus might be involved in the expression of GM2 in cervical carcinoma-derived cells.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Enhanced expression of unique gangliosides with GM2-determinant in human uterine cervical carcinoma-derived cell lines

et al. 2016

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“…[27][28][29] There is also a growing interest in the role of glycolipids in bacterial and viral infection and therefore the potential for glycolipid-based therapeutic strategies. [30][31][32][33] Here we present experiments demonstrating a protected synthetic glycolipid, with a galactose-based headgroup and two asymmetrical hydrocarbon tails, has been successfully incorporated into phospholipid-cholesterol giant unilamellar vesicles (GUVs) at biologically relevant concentrations. The glycolipid-containing liposomes show good stability at room temperature.…”

Section: Introductionmentioning

confidence: 99%

Adding glycolipid functionality to model membranes – phase behaviour of a synthetic glycolipid in a phospholipid membrane

et al. 2014

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Glycolipid phase behaviour is less well understood than for many phospholipids, but due to their structural and functional diversity, glycolipids represent an important group of amphiphiles from which biological function is derived. Here we have incorporated a synthetic glycolipid in binary mixtures with DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine) into giant unilamellar vesicles (GUVs) at biologically relevant concentrations and observed the phase behaviour of the lipid mixtures for a range of glycolipid concentrations. At low concentrations, the glycolipid is fully dispersed in the GUV membrane. At glycolipid molar concentrations above 10%, the formation of lipid tubules is observed, and is consistent with the formation of a columnar lipid phase. Lipid tubules are observed in aqueous and oil solvents, suggesting that both hexagonal and inverted hexagonal lipid arrangements can be formed. This work may offer insights into the biological function of glycolipids and the challenges in formulating them for use in industrial applications.

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Changes in bacterial glycolipids as an index of intestinal lactobacilli and epithelial glycolipids in the digestive tracts of mice after administration of penicillin and streptomycin

Iwamori

Adachi

et al. 2013

Glycoconj J

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The major lipid constituent of symbiotic gram-positive bacteria in animals are phosphatidylglycerol, cardiolipin and dihexaosyl diglycerides (DH-DG), whose hydrophobic structures are characteristic of the environments, and the carbohydrate structures of DH-DGs are bacterial species-characteristic. Immunization of rabbits with intestinal lactobacilli generated antibodies against DH-DGs and their modified structures, among which Galα1-6-substituted DH-DG, i.e., Lactobacillus tetrahexaosyl diglyceride (LacTetH-DG), reacted with antibodies more intensely than DH-DG. Whereas, from the 16S-rRNA sequence, the intestinal lactobacilli in murine digestive tracts were revealed to be L. johnsonii, in which LacTetH-DG is present at the concentration of 2.2 ng per 1 × 10(6) cells. To obtain more accurate estimates of intestinal lactobacilli in several regions of the digestive tract of mice, LacTetH-DG was detected by TLC-immunostaining with anti-Lactobacillus antisera, being found in the stomach, cecum and colon of normal breeding mice, 1.0 × 10(9), 3.5 × 10(9) and 7.4 × 10(9) cells, respectively. Administration of penicillin and streptomycin for 6 days resulted in a reduction in the number of intestinal lactobacilli, the levels being 0 %, 30 % and 4 % of the control ones in the stomach, cecum and colon, respectively, which was associated with the accumulation of the contents in the tracts from the stomach to the cecum and with diarrhea. In addition, a reduced amount of fucosyl GA1 (FGA1) and a compensatory increase in GA1 due to the reduced activity of α1,2-fucosyltransferase in the small intestine and the enhanced discharge of FGA1 into the contents occurred in mice, probably due to the altered population of bacteria caused by administration of penicillin and streptomycin.

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Bacterial species-characteristic profiles of molecular species, and the antigenicity of phospholipids and glycolipids in symbiotic Lactobacillus, Staphylococcus and Streptococcus species

Cited by 7 publications

References 24 publications

Enhanced expression of unique gangliosides with GM2-determinant in human uterine cervical carcinoma-derived cell lines

Enhanced expression of unique gangliosides with GM2-determinant in human uterine cervical carcinoma-derived cell lines

Adding glycolipid functionality to model membranes – phase behaviour of a synthetic glycolipid in a phospholipid membrane

Changes in bacterial glycolipids as an index of intestinal lactobacilli and epithelial glycolipids in the digestive tracts of mice after administration of penicillin and streptomycin

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