Bacterial stress defense: the crucial role of ribosome speed

Zhu, Manlu; Dai, Xiongfeng

doi:10.1007/s00018-019-03304-0

Cited by 24 publications

(19 citation statements)

References 58 publications

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“…To attenuate oxygen toxicity and ensure cell survival, timely transcriptional changes to protein modifications are necessary to activate the synthesis of defense proteins (Zhu and Dai, 2019). The translational capacity is then controlled by the ribosomal proteins (r-proteins), which are important components of the translational machinery.…”

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confidence: 99%

Short communication: Modulation of fatty acid metabolism improves oxygen tolerance of Bifidobacterium animalis ssp. lactis Probio-M8

Zhang

Wang

et al. 2020

Journal of Dairy Science

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Bifidobacterium animalis ssp. lactis Probio-M8 is a potential probiotic strain that was isolated from human milk. Previously, we obtained an oxygen-resistant variant (Probio-M8o) of Probio-M8 by an adaptive evolution strategy. In the present study, a comparative transcriptomic analysis of Probio-M8o and Probio-M8 was carried out to reveal the cellular mechanism of the oxygen-resistant phenotype. Using RNA-seq, 210 and 217 differentially expressed genes were identified in Probio-M8o compared with Probio-M8 after oxygen exposure for 30 and 60 min, respectively. The oxygen treatment upregulated a set of genes that encoded proteins responsible for fatty acid biosynthesis. This observation was in good agreement with the composition change in fatty acids at the biochemical level. Our study showed that the oxygen-resistant phenotype could be related to adaptation of fatty acid metabolism.

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confidence: 99%

Short communication: Modulation of fatty acid metabolism improves oxygen tolerance of Bifidobacterium animalis ssp. lactis Probio-M8

Zhang

Wang

et al. 2020

Journal of Dairy Science

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“…3 ; Data set S1). Protein synthesis lies at the core of cell growth, and the ribosomal content exhibited positive linear correlations with the growth rate in the medium without restricted factors ( 35 ). However, it is difficult for bacteria to maintain the optimal state due to various limitations.…”

Section: Resultsmentioning

confidence: 99%

Water-Soluble Humic Materials Modulating Metabolism and Triggering Stress Defense in Sinorhizobium fredii

et al. 2021

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“…Under adverse conditions, bacterial cells maintain an inactive ribosome pool so they can quickly resume growth when environmental conditions become favourable. Since stress‐related genes are preferentially transcribed during stress, high‐abundance mRNAs naturally attract a large number of ribosomal translation‐related proteins (Zhu et al ., 2020). Upregulated expression of elongation factor Tu ( tuf ) promotes GTP‐dependent binding of aminoacyl‐tRNA to ribosome site A during protein biosynthesis, like heat shock proteins.…”

Section: Resultsmentioning

confidence: 99%

iTRAQ‐based quantitative proteomic analysis of the effect of heat shock on freeze‐drying of Lactobacillusacidophilus ATCC4356

Liu

Zeng

et al. 2021

Int J of Food Sci Tech

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Summary In order to investigate the mechanism by which heat shock improves the survival rate of lactic acid bacteria (LAB) following lyophilisation, we performed proteomic analysis based on isobaric tags for relative and absolute quantitation (iTRAQ) on Lactobacillus acidophilus (LA) ATCC4356. Among the 75 differentially expressed proteins (DEPs), 16 and 59 were up‐ and downregulated, respectively, mainly related to amino acid metabolism, glycolysis, ABC transportation, transcription and translation. MrnC, tuf, recG, ychF, purB, dtpT, LBA1220, LBA0434 and LBA0921 were identified as key genes mediating tolerance to freeze‐drying. Energy supply was enhanced by regulating amino acid metabolism and glycolysis, while energy consumption was decreased by downregulating most translation‐related proteins. DNA and the cellular internal environment were stabilised, and ABC transporters assisted adaptation to freeze‐drying. The results provide new insight for understanding tolerance to freeze‐drying in LAB following heat shock treatment.

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Bacterial stress defense: the crucial role of ribosome speed

Cited by 24 publications

References 58 publications

Short communication: Modulation of fatty acid metabolism improves oxygen tolerance of Bifidobacterium animalis ssp. lactis Probio-M8

Short communication: Modulation of fatty acid metabolism improves oxygen tolerance of Bifidobacterium animalis ssp. lactis Probio-M8

Water-Soluble Humic Materials Modulating Metabolism and Triggering Stress Defense in Sinorhizobium fredii

iTRAQ‐based quantitative proteomic analysis of the effect of heat shock on freeze‐drying of Lactobacillusacidophilus ATCC4356

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