Bacterial Toxicity of Functionalized Polystyrene Latex Nanoparticles Toward &lt;i&gt;Escherichia coli&lt;/i&gt;

Miyazaki, Jumpei; Kuriyama, Yuta; Miyamoto, Akihisa; Tokumoto, Hayato; Konishi, Yasuhiro; Nomura, Toshiyuki

doi:10.4028/www.scientific.net/amr.699.672

Cited by 8 publications

(14 citation statements)

References 14 publications

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“…These PS NP are biocompatible and stable in cell culture media. Reportedly, plain and surface modified PS NP with an overall negative charge have no cytotoxic effect on E. coli 35 , 36 . To support this assumption, a live-dead staining of the biofilm was performed after 24 h of incubation of the PS NP used for the preparation of the nanosensor (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 96%

Monitoring and imaging pH in biofilms utilizing a fluorescent polymeric nanosensor

Kromer

Schwibbert

Gadicherla

et al. 2022

Sci Rep

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Biofilms are ubiquitous in nature and in the man-made environment. Given their harmful effects on human health, an in-depth understanding of biofilms and the monitoring of their formation and growth are important. Particularly relevant for many metabolic processes and survival strategies of biofilms is their extracellular pH. However, most conventional techniques are not suited for minimally invasive pH measurements of living biofilms. Here, a fluorescent nanosensor is presented for ratiometric measurements of pH in biofilms in the range of pH 4.5–9.5 using confocal laser scanning microscopy. The nanosensor consists of biocompatible polystyrene nanoparticles loaded with pH-inert dye Nile Red and is surface functionalized with a pH-responsive fluorescein dye. Its performance was validated by fluorometrically monitoring the time-dependent changes in pH in E. coli biofilms after glucose inoculation at 37 °C and 4 °C. This revealed a temperature-dependent decrease in pH over a 4-h period caused by the acidifying glucose metabolism of E. coli. These studies demonstrate the applicability of this nanosensor to characterize the chemical microenvironment in biofilms with fluorescence methods.

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Section: Resultsmentioning

confidence: 96%

Monitoring and imaging pH in biofilms utilizing a fluorescent polymeric nanosensor

Kromer

Schwibbert

Gadicherla

et al. 2022

Sci Rep

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show abstract

“…These PS NP are biocompatible and stable in cell culture media. Reportedly, plain and surface modi ed PS NP with an overall negative charge have no cytotoxic effect on E. coli 35,36 . To support this assumption, a live-dead staining of the bio lm was performed after 24 hours of incubation of the PS NP used for the preparation of the nanosensor (Supporting Figure S3).…”

Section: Resultsmentioning

confidence: 99%

Monitoring And Imaging Ph In Biofilms Utilizing A Fluorescent Polymeric Nanosensor

Kromer

Schwibbert

Gadicherla

et al. 2022

Preprint

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Biofilms are ubiquitous in nature and in the man-made environment. Given their harmful effects on human health, an in-depth understanding of biofilms and the monitoring of their formation and growth are important. Particularly relevant for many metabolic processes and survival strategies of biofilms is their extracellular pH. However, most conventional techniques are not suited for minimally invasive pH measurements of living biofilms. Here, a fluorescent nanosensor is presented for ratiometric measurements of pH in biofilms in the range of pH 4.5-9.5 using confocal laser scanning microscopy. The nanosensor consists of biocompatible polystyrene nanoparticles loaded with pH-inert dye Nile Red and is surface functionalized with a pH-responsive fluorescein dye. Its performance was validated by fluorometrically monitoring the time-dependent changes in pH in E. coli biofilms after glucose inoculation at 37°C and 4°C. This revealed a temperature-dependent decrease in pH over a 4-hour period caused by the acidifying glucose metabolism of E. coli. These studies demonstrate the applicability of this nanosensor to characterize the chemical microenvironment in biofilms with fluorescence methods.

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“…These microplastics can break down into nano-sized particles (~ 100 nm) through chemical and biological processes and become available for ingestion by zooplankton, fishes, bivalves, mussels, nematodes and crustaceans 4 – 9 . They may also be toxic to bacteria 10 – 13 . Plastic particles are capable of adsorbing multiple substances present in water, which affects their toxicity potential, as chemicals are concentrated and/or the particles become a vector for the spread of contamination 14 – 17 .…”

Section: Introductionmentioning

confidence: 99%

“…Also, the expanded PS containing 98% of air and developed as a low-density material for single-time uses is easily carried by winds and water and can increase the level of polymer contamination 43 – 45 . Multiple studies have examined the intrinsic toxicities of different PS particles toward bacteria 10 , 13 , 46 . PS has been shown to inhibit cell growth of the marine bacteria Halomonas alkaliphila 10 , control periphytic biofilm formation 47 and increase antibacterial activity against E. coli due to exposure to UV radiation 46 .…”

Section: Introductionmentioning

confidence: 99%

“…Those particles did have a toxic effect on V. fischeri ; the observed toxic effects were linked to surfactants and additives present in PS particles. Functional modifications of PS particles' surfaces may decrease the viability of E. coli cells 13 . Specifically, cationic amino-functionalized PS particles can lead to E. coli membrane destabilization and a ROS-mediated toxic effect 11 .…”

Section: Introductionmentioning

confidence: 99%

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Submicron polymer particles may mask the presence of toxicants in wastewater effluents probed by reporter gene containing bacteria

Bhuvaneshwari

Eltzov

Borisover

2021

Sci Rep

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Microplastics are ubiquitous in aquatic systems and break down into submicron particles that can interact with aquatic toxic chemicals. These interactions may affect the detection of toxicants when using bacteria as a biomonitoring tool. This study examined the effects of model polystyrene (PS)-based submicron particles on the detection of aqueous geno- and cytotoxicity by genetically modified bioluminescent (GMB) bacteria. The toxicities were tested in three treated wastewater (TWW) effluents before and after chlorination. The PS plastics included negatively charged sulfate-coated (S-PS) and pristine (P-PS) particles of different sizes (0.1, 0.5, and 1.0 µm) that were present at different concentrations. Chlorinated or not, the S-PS and P-PS particles per se were not toxic to the GMB bacteria. However, exposure of PS particles to TWW effluents can significantly reduce the measured geno- and cytotoxicity. Adsorption of toxic compounds to polymer particles can limit the ability of the bacteria to detect those compounds. This masking effect may be mitigated by TWW chlorination, possibly due to the formation of new toxic material. Due to interactions between toxic TWW constituents and the plastics particles, water samples containing particle-associated contaminants and/or their transformation products may be declared non-toxic, based on bacterial tests as a biomonitoring tool.

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Bacterial Toxicity of Functionalized Polystyrene Latex Nanoparticles Toward <i>Escherichia coli</i>

Cited by 8 publications

References 14 publications

Monitoring and imaging pH in biofilms utilizing a fluorescent polymeric nanosensor

Monitoring and imaging pH in biofilms utilizing a fluorescent polymeric nanosensor

Monitoring And Imaging Ph In Biofilms Utilizing A Fluorescent Polymeric Nanosensor

Submicron polymer particles may mask the presence of toxicants in wastewater effluents probed by reporter gene containing bacteria

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