Bacterial translocation across ePTFE vascular graft surfaces

Abstract

Summary Objectives Vascular graft infections arise from bacterial colonization of either the external or internal graft surfaces. We assessed whether methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli could translocate through pores of ePTFE grafts. Methods To assess translocation from the internal to the external surface, we placed 105 cfu of bacterial suspension inside ePTFE graft segments and suspended them in sterile broth for 72 h. To assess translocation from the external to the internal surface, we placed sterile broth inside ePTFE segments, and incubated them for 72 h in a bacterial suspension (105 cfu/mL). At 72 h, in addition to culturing the sterile broth and bacterial suspensions, the external and internal surfaces were first qualitatively cultured separately and then quantitatively cultured by sonication. Results At 72 h, the sterile broth remained sterile. The bacterial suspensions yielded 107–109 cfu/mL. Graft cultures indicated that colonization of one surface with either organism did not result in bacterial translocation to the other surface. Quantitative bacterial counts of the external vs. internal surfaces were significantly different (p < 0.01). Conclusions MRSA and E. coli do not translocate across ePTFE graft surfaces. These in-vitro findings help elucidate the pathogenesis of graft infections and prompt conduction of validation studies in-vivo.

Autologous Vascularization: A Method to Enhance the Antibacterial Adhesion Properties of ePTFE

“…15,16 However, numerous clinical reports showed that bacterial infection is one of the most common complications after biomaterial implantation, and the underlying mechanism is mainly due to sustained secretion of inflammatory factors and reduction in the ability of local tissue cells to remove bacteria after long-term use. 17,18 More importantly, the formation of biofilm on a biomaterial surface by bacterial adhesion has strong drug resistance and resistance to phagocytosis. Current studies show that modifications by covering with a bioactive factor or biomaterial surfaces prevascularized in vitro exhibit a low bacterial adhesion rate, improving the antiinfection ability of implanted materials.…”

Endovascular treatment for mycotic aneurysm using pyoktanin- applied devices

“…In surgery for MTAA, expanded polytetrafluoroethylene (ePTFE) grafts and RFP-soaked ePTFE grafts have been used, and there are reports on such usage. Narasimhan et al reported that ePTFE graft did not allow methicillin-resistant Staphylococcus aureus (MRSA) and E. coli to pass through the vessel wall, even when their biofilm was formed on the vessel surface (Narasimhan et al 2010 ). As far as our research is concerned, there were no reports of increased postsurgical infections between the use of ePTFE graft and non ePTFE grafts (Taichi et al 2016 ; Hai Lei and Yui Che 2018 ).…”

Characteristics of bacterial biofilm associated with implant material in clinical practice

“…79 Although it is believed that the translocation of bacterial cells along the pores of the graft has an important role in infection, methicillin-resistant Staphylococcus aureus and Escherichia coli do not exhibit such a behavior. 80…”