Bactericidal action of nafcillin, vancomycin, and three cephalosporins against nafcillin-susceptible and nafcillin-resistant coagulase-negative staphylococci

Antipseudomonal activity of simulated infusions of gentamicin alone or with piperacillin assessed by serum bactericidal rate and area under the killing curve

“…Unlike the timed killing curve method (18) and the checkerboard technique (2), which are performed in broth medium, the SBR assay allows consideration of both pharmacokinetics and serum protein binding of the antimicrobial agent(s) under study (5). Briceland et al (5) modified the SBR assay of Drake et al (7) in such a way as to quantitate the rate of killing; Mordenti et al (26) have published a similar method. The latter approach allows for statistical comparison of rates of killing of antimicrobial agents rather than the application of arbitrary criteria for such comparisons.…”

“…Gentamicin alone or combined with piperacillin was added to heat-inactivated human serum to approximate drug concentrations achieved after the above-mentioned types of infusion. By a microdilution technique, seven strains of P. aeruginosa were exposed to no drug (control) Recently, the rate of serum bacterial killing, or serum bactericidal rate (SBR), has been investigated as a measure of the antimicrobial activity of antibiotics alone and in combination (5,7,26,29,(33)(34)(35). The SBR assay, first described by Drake et al (7), is an abbreviated timed killing curve performed in serum, rather than broth.…”