Bactericidal effect of photodynamic inactivation against methicillin-resistant and methicillin-susceptible Staphylococcus aureus is strain-dependent

Grinholc, Mariusz; Szramka, Bozena; Kurlenda, Julianna; Graczyk, Alfreda; Bielawski, Krzysztof Piotr

doi:10.1016/j.jphotobiol.2007.11.002

Cited by 119 publications

(99 citation statements)

References 28 publications

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“…Besides, it should be pointed out that the microbial reduction over 95% (log reduction % 2) obtained using the PS concentration of 50 or 75 lg/ml and 0.8 J/cm 2 light dose are in accordance with the results obtained with other conventional microbial reduction techniques [8,25,28]. It is clear that a higher microorganism reduction can be obtained with the much higher light intensities achieved with laser or LED sources, as presented by studies where the log decrease is of 4-6 units [2,29,33].…”

Section: Discussionsupporting

confidence: 83%

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Chemiluminescence as a PDT light source for microbial control

Ferraz

Fontana

Ribeiro

et al. 2011

Journal of Photochemistry and Photobiology B: Biology

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Section: Discussionsupporting

confidence: 83%

“…PDT is one of the alternative procedures and based on its mechanisms, it is not likely that microorganisms develop resistance [7,8].…”

Section: Introductionmentioning

confidence: 99%

Chemiluminescence as a PDT light source for microbial control

Ferraz

Fontana

Ribeiro

et al. 2011

Journal of Photochemistry and Photobiology B: Biology

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“…46 Furthermore, the strain dependent differences of bactericidal photodynamic inactivation could not be correlated either to the levels of photosensitizer uptake or the pharmacological inhibition of the efflux pump. 47 Therefore other factors might be influencing such observed differences in the photodynamic efficacy such as aggregation of the photosensitizer, concentration of antioxidant enzymes or cellular repair proteins, but all these factors are not responsible to diminish TMPyP induced photosensitization, because up to now there is a lack of selection of photoresistant bacteria after multiple photodynamic treatments.…”

Section: Photochemical and Photobiological Sciences Papermentioning

confidence: 94%

Dirty hands: photodynamic killing of human pathogens like EHEC, MRSA and Candida within seconds

Eichner

Gonzales

Felgenträger

et al. 2012

Photochem Photobiol Sci

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aHand hygiene is one of the most important interventions for reducing transmission of nosocomial lifethreatening microorganisms, like methicillin resistant Staphylococcus aureus (MRSA), enterohemorrhagic Escherichia coli (EHEC) or Candida albicans. All three pathogens have become a leading cause of infections in hospitals. Especially EHEC is causing severe diarrhoea and, in a small percentage of cases, haemolytic-uremic syndrome (HUS) as reported for E. coli 104:H4 in Germany 2011. We revealed the possibility to inactivate very fast and efficiently MRSA, EHEC and C. albicans using the photodynamic approach.MRSA, EHEC and C. albicans were incubated in vitro with different concentrations of TMPyP for 10 s and illuminated with visible light (50 mW cm TMPyP on ex vivo porcine skin, fluorescence microscopy of histology showed that the photosensitizer was exclusively localized in the stratum corneum regardless of the incubation time. TMPyP exhibited a fast and very effective killing rate of life-threatening pathogens within a couple of seconds that encourages further testing in an in vivo setting. Being fast and effective, antimicrobial photodynamic applications might become acceptable as a tool for hand hygiene procedures and also in other skin areas.

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“…us, in vitro studies have concentrated more on bio�lm models as well as in vivo models, particularly rat and mouse models of infected wounds ( Table 2). As we have discussed previously, when studying the use of photoinactivation in bio�lm-related models, the mechanism of strain-dependent response to PDI requires further investigation [50][51][52][53].…”

Section: Antimicrobial Photodynamic Therapymentioning

confidence: 99%

Antimicrobial, Antimycobacterial and Antibiofi lm Properties of Couroupita guianensis Aubl. Fruit Extract

Sayen¹

2014

Biofilm Control and Antimicrobial Agents

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We review the recent literature concerning the efficiency of antimicrobial photodynamic inactivation toward various microbial species in planktonic and bio�lm cultures. e review is mainly focused on bio�lm-growing microrganisms because this form of growth poses a threat to chronically infected or immunocompromised patients and is difficult to eradicate from medical devices. We discuss the bio�lm formation process and mechanisms of its increased resistance to various antimicrobials. We present, based on data in the literature, strategies for overcoming the problem of bio�lm resistance. �actors that have potential for use in increasing the efficiency of the killing of bio�lm-forming bacteria include plant extracts, enzymes that disturb the bio�lm structure, and other nonenzymatic molecules. We propose combining antimicrobial photodynamic therapy with various antimicrobial and antibio�lm approaches to obtain a synergistic effect to permit efficient microbial growth control at low photosensitizer doses.

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Bactericidal effect of photodynamic inactivation against methicillin-resistant and methicillin-susceptible Staphylococcus aureus is strain-dependent

Cited by 119 publications

References 28 publications

Chemiluminescence as a PDT light source for microbial control

Chemiluminescence as a PDT light source for microbial control

Dirty hands: photodynamic killing of human pathogens like EHEC, MRSA and Candida within seconds

Antimicrobial, Antimycobacterial and Antibiofi lm Properties of Couroupita guianensis Aubl. Fruit Extract

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