Bactericidal Effects and Mechanisms of Visible Light-Responsive Titanium Dioxide Photocatalysts on Pathogenic Bacteria

Liou, Je-Wen; Chang, Hsin-Hou

doi:10.1007/s00005-012-0178-x

Cited by 179 publications

(136 citation statements)

References 72 publications

(92 reference statements)

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“…7). This was already observed for E. coli cells [35]. For Listeria, adherent cells onto TiO 2 have broken their flagella and the cytoplasm is released (Fig.…”

Section: Discussionsupporting

confidence: 68%

Antimicrobial Activity of Stainless Steel with a Modified TiN Upperlayer on Meat Related Contaminants

Nardi

Delaunay

Talibart

et al. 2016

JFSE

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Advanced material with antibacterial properties would be a promising way to improve the disinfection process in food plants. Our objective was to combine the bactericidal effect of TiO 2 with the mechanical strength of TiN coatings. A TiO 2 rutile film was obtained after annealing of a supplied 316 stainless steel with a TiN coating. This TiO 2 upperlayer displays a photocatalytic activity under UV light exposure. The substrates with the TiN coating and the TiO 2 upperlayer are more hydrophobic than the 316 control. The adhesion of either Listeria or Pseudomonas, on 316-TiN is characterized by the presence of clusters of cells, while the oxidation of the TiN surface leads to a more hydrophilic layer where cells are individualized. After UV illumination of the adherent cells and subsequent growth, the residual bacterial population present on 316-TiO 2 is lower than that present on the 316-TiN. The bactericidal effect is more important on Listeria than on Pseudomonas.

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“…7). This was already observed for E. coli cells [35]. For Listeria, adherent cells onto TiO 2 have broken their flagella and the cytoplasm is released (Fig.…”

Section: Discussionsupporting

confidence: 68%

Antimicrobial Activity of Stainless Steel with a Modified TiN Upperlayer on Meat Related Contaminants

Nardi

Delaunay

Talibart

et al. 2016

JFSE

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“…We used the commercially available P25 titania nanoparticles with an average diameter of 25 nm (30). There have been many innovative approaches using nanotechnology to improve the effectiveness of titania photocatalysis.…”

Section: Discussionmentioning

confidence: 99%

Broad-Spectrum Antimicrobial Effects of Photocatalysis Using Titanium Dioxide Nanoparticles Are Strongly Potentiated by Addition of Potassium Iodide

Huang

Choi

Kushida

et al. 2016

Antimicrob Agents Chemother

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f Photocatalysis describes the excitation of titanium dioxide nanoparticles (a wide-band gap semiconductor) by UVA light to produce reactive oxygen species (ROS) that can destroy many organic molecules. This photocatalysis process is used for environmental remediation, while antimicrobial photocatalysis can kill many classes of microorganisms and can be used to sterilize water and surfaces and possibly to treat infections. Here we show that addition of the nontoxic inorganic salt potassium iodide to TiO 2 (P25) excited by UVA potentiated the killing of Gram-positive bacteria, Gram-negative bacteria, and fungi by up to 6 logs. The microbial killing depended on the concentration of TiO 2 , the fluence of UVA light, and the concentration of KI (the best effect was at 100 mM). There was formation of long-lived antimicrobial species (probably hypoiodite and iodine) in the reaction mixture (detected by adding bacteria after light), but short-lived antibacterial reactive species (bacteria present during light) produced more killing. Fluorescent probes for ROS (hydroxyl radical and singlet oxygen) were quenched by iodide. Tri-iodide (which has a peak at 350 nm and a blue product with starch) was produced by TiO 2 -UVA-KI but was much reduced when methicillin-resistant Staphylococcus aureus (MRSA) cells were also present. The model tyrosine substrate N-acetyl tyrosine ethyl ester was iodinated in a light dose-dependent manner. We conclude that UVA-excited TiO 2 in the presence of iodide produces reactive iodine intermediates during illumination that kill microbial cells and long-lived oxidized iodine products that kill after light has ended. Heterogeneous photocatalysis is the use of photoactivated titanium dioxide to produce reactive oxygen species in order to destroy organic pollutants and to kill different classes of microorganisms (1). TiO 2 is a wide-band gap semiconductor, and when a photon of sufficient energy is absorbed, an electron is excited from the valence band to the conduction band, generating a positive hole in the valence band (2). Since energy levels are not available to promote ready recombination of the electron and the hole (as they are in metallic conductors), the electrons and holes survive long enough to carry out reactions. The electrons can reduce oxygen to superoxide, while at the same time the holes can oxidize water to hydroxyl radicals (3). Hydrogen peroxide and singlet oxygen are also produced (4).Although TiO 2 can occur in several different crystalline forms (anatase, rutile, and brookite), the anatase form is usually employed for applications in photocatalysis (5). Because the process is heterogeneous, TiO 2 nanoparticles that have the maximum surface area/mass ratio are optimum for efficient catalytic activity. The maximum absorption wavelength of the TiO 2 nanoparticle is about 360 nm (6), equivalent to the semiconductor band gap of 3.35 eV (7). The preparation of TiO 2 known as Degussa or Aeroxide (Evonik) P25 is composed of 21-nm-diameter nanoparticles, which are about 75% anatase, 1...

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“…This could be attributed to the visible light irradiation that provides minimal energy to create electrons and promotes the electrons in the codoped-TiO 2 to travel from valence band to the conduction band. The energy generated by visible light irradiation is not strong enough to activate pure TiO 2 samples [31][32][33].…”

Section: Microbe-free Surfacesmentioning

confidence: 99%

Antimicrobial Double-Layer Coating Prepared from Pure or Doped-Titanium Dioxide and Binders

Jin

Liu

et al. 2018

Coatings

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Abstract:Fruit and vegetable containers with microbe-free surfaces can be made by coating with titanium dioxide (TiO 2 ) particles or nonmetal (C, N, B, F) doped-TiO 2 particles, using wear resistant polymers, such as zein, and paint, as the binders and to form a continuous binding phase. The doped-TiO 2 powders absorb visible light radiation, and thus possess a higher antibacterial effect than non-modified TiO 2 particles in environmental conditions. The study also presents a double-layer coating to use less TiO 2 particles in coating, while achieving higher antimicrobial activity. Containers with microbe-free surfaces can stop cross-contamination from infected workers or spoiled/decayed/contaminated fruits or vegetables, and thus are expected to be able to reduce the risk from microbiological contamination of fruits and vegetables during harvest in fields, and postharvest storage or transportation.

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Bactericidal Effects and Mechanisms of Visible Light-Responsive Titanium Dioxide Photocatalysts on Pathogenic Bacteria

Cited by 179 publications

References 72 publications

Antimicrobial Activity of Stainless Steel with a Modified TiN Upperlayer on Meat Related Contaminants

Antimicrobial Activity of Stainless Steel with a Modified TiN Upperlayer on Meat Related Contaminants

Broad-Spectrum Antimicrobial Effects of Photocatalysis Using Titanium Dioxide Nanoparticles Are Strongly Potentiated by Addition of Potassium Iodide

Antimicrobial Double-Layer Coating Prepared from Pure or Doped-Titanium Dioxide and Binders

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