Bactericidal Effects of Exiguobacterium sp GM010 Pigment Against Food-Borne Pathogens

Mekala, Krishnaprashanth Ramesh; Pandit, Saritha Gopal; Dhale, Mohan Appasaheb

doi:10.3389/fsufs.2020.00142

Cited by 11 publications

(4 citation statements)

References 31 publications

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“…It has antagonistic effect on a variety of plant pathogenic fungi. It shows the ability of nitrogen fixation, phosphate solubilization and iron carrier generation [70][71] . The relative abundance of Weissella in leaves is high, which is beneficial bacteria 72 .…”

Section: Discussionmentioning

confidence: 99%

Effects of Rare Earth Elements on Bacteria in Rhizosphere, Root, Phyllosphere and Leaf of Soil-Rice Ecosystem

Xin-zhuan

Pan

et al. 2021

Preprint

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The effects of rare earth mining on rice biomass, rare earth element(REE) content and bacterial community structure was studied by pot experiment. The research showed that the REE content in rice roots, shoots and grains was significantly positive correlated with that in soil, and the dry weight of rice roots, shoots and grains was highly correlated with soil physical and chemical properties, nutrient elements and REE contents; The exploitation of rare earth minerals inhibited a-diversity of endophytic fungi in rhizosphere, root, phyllosphere and leaf of rice, significantly reduced the abundance index, OTU number, Chao, Ace index and also significantly reduced the diversity index-Shannon index and Inv-Simpson index, also reduced uniformity index: Pielou’s evenness index, Simpson’s evenness index, etc, which caused β-diversity of bacteria to be quite different. The exploitation of rare earth minerals reduces the diversity of bacteria, but forms dominant bacteria, such as Burkholderia, Bacillus, Buttiauxella, Acinetobacter, Bradyrhizobium, Candida koribacter, which can degrade the pollutants formed by exploitation of rare earth minerals, alleviate the compound pollution of rare earth and ammonia nitrogen, and also has the function of fixing nitrogen and resisting rare earth stress; The content of soil available phosphorus in non mining area is low, forming the dominant bacteria of Pantoea, which has the function of improving soil phosphorus availability. Rare earth elements and physical and chemical properties of soil affect the community structure of bacteria in rhizosphere and phyllosphere of rice, promote the parallel movement of some bacteria in rhizosphere, root, phyllosphere and leaf of rice, promote the construction of community structure of bacteria in rhizosphere and phyllosphere of rice, give full play to the growth promoting function of Endophytes, and promote the growth of rice. The results showed that the rice biomass in mining area was guaranteed due to the growth promoting effect of endophytic bacteria, However, the mining of mineral resources causes the compound pollution of rare earth and ammonia nitrogen, which makes REE content of rice in mining area significantly higher than that in non mining area.

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Section: Discussionmentioning

confidence: 99%

Effects of Rare Earth Elements on Bacteria in Rhizosphere, Root, Phyllosphere and Leaf of Soil-Rice Ecosystem

Xin-zhuan

Pan

et al. 2021

Preprint

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“…Exiguobacterium sp. (NCBI, KY014429), isolated previously in our laboratory [11], was used in this study. The culture streaked on Zobell Marine Agar (ZMA) slants was maintained at 4°C with subculturing at regular and successive periods.…”

Section: Microorganisms and Substratementioning

confidence: 99%

“…The pigmented Exiguobacterium sp GM010 biomass did not show signi cant toxic signs during the acute toxicity study. The pigment produced by Exiguobacterium sp GM010 has been reported to possess antioxidant, antibacterial and anticancer properties [11,12].…”

Section: Introductionmentioning

confidence: 99%

Valorisation of prawn carapace waste by Exiguobacterium sp GM010 for pilot scale pigment production and its safety efficacy in Wistar rats

Mekalaa,

Peddha,

DHALE

2023

Preprint

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Purpose Utilisation of prawn carapace hydrolysate by Exiguobacterium sp GM010 for the growth and bioactive pigment production as fermentative substrate. To produce the pigment in pilot scale, the culture conditions were optimized and safety efficacy studies of the pigment were evaluated for commercial application in food and nutraceutical industries. Methods Exiguobacterium sp GM010 was grown in prawn carapace (biowaste) hydrolysate as fermentative substrate in a 10L medium using a 15L capacity fermentor. Based on our previous studies, fermentation parameters were set (pH 8.0, 30˚C, five days) by varying the aeration (0.5 to 3.0L min− 1). The pigment produced was quantitatively and qualitatively analysed in comparison with pigment produced in lab scale fermentation. Both acute (OECD 423) and sub-acute (OECD 427) toxicity studies were performed to determine its safety efficacy. Results The biomass and pigment extract yield were 28.76 ± 1.78 and 0.82 ± 0.04 g/L, respectively, at 1.5 L of aeration and higher than other aeration concentrations. The UV-visible spectrum of pigment extract revealed the absorption peak at 357, 466, and 491nm. The colour distribution of pigment was in the yellowish-orange region of the CIE Lab spectrum. Similar qualitative and quantitative pigment characteristics were observed in the fermentor and flask. The acute and sub-acute toxicity of the pigment extract confirmed no significant changes in feed intake, body weight (bw) gain and relative vital organ weight in Wistar rats. Similarly, no significant changes in biochemical and haematological parameters at 250, 500 and 1000 mg kg− 1 bw confirmed no sign of toxicity. Histopathological examination of all the vital organs was regular. Serum biochemistry results of the treated and control groups have shown insignificant changes. Conclusion Prawn carapace hydrolysate can be valorised on a pilot scale to produce Exiguobacterium sp GM010 pigment. Further, the pigments so produced were found to be non-toxic to Wistar rats, indicating its potential use as food additives and nutraceuticals.

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“…Food-borne illnesses are considered a major concern globally. The World Health Organization (WHO) has reported approximately 600 million food-borne illnesses and 420,000 deaths due to 31 global hazards (major pathogens) [1,2]. In particular, numerous food-borne illnesses caused by fresh produce consumption, mainly raw vegetables and fruits contaminated by pathogenic bacteria, have been reported.…”

Section: Introductionmentioning

confidence: 99%

Microbiota Analysis and Microbiological Hazard Assessment in Chinese Chive (Allium tuberosum Rottler) Depending on Retail Types

Seo

Yum

Lee

et al. 2022

J. Microbiol. Biotechnol.

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Chinese chive ( Allium tuberosum Rottler) has potential risks associated with pathogenic bacterial contamination as it is usually consumed raw. In this study, we investigated the microbiota of Chinese chives purchased from traditional markets and grocery stores in March (Spring) and June (Summer) 2017. Differences in bacterial diversity were observed, and the microbial composition varied across sampling times and sites. In June, potential pathogenic genera, such as Escherichia , Enterobacter , and Pantoea , accounted for a high proportion of the microbiota in samples purchased from the traditional market. A large number of pathogenic bacteria ( Acinetobacter lwoffii , Bacillus cereus , Klebsiella pneumoniae , and Serratia marcescens ) were detected in the June samples at a relatively high rate. In addition, the influence of the washing treatment on Chinese chive microbiota was analyzed. After storage at 26°C, the washing treatment accelerated the growth of enterohemorrhagic Escherichia coli (EHEC) because it caused dynamic shifts in Chinese chive indigenous microbiota. These results expand our knowledge of the microbiota in Chinese chives and provide data for the prediction and prevention of food-borne illnesses.

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Bactericidal Effects of Exiguobacterium sp GM010 Pigment Against Food-Borne Pathogens

Cited by 11 publications

References 31 publications

Effects of Rare Earth Elements on Bacteria in Rhizosphere, Root, Phyllosphere and Leaf of Soil-Rice Ecosystem

Effects of Rare Earth Elements on Bacteria in Rhizosphere, Root, Phyllosphere and Leaf of Soil-Rice Ecosystem

Valorisation of prawn carapace waste by Exiguobacterium sp GM010 for pilot scale pigment production and its safety efficacy in Wistar rats

Microbiota Analysis and Microbiological Hazard Assessment in Chinese Chive (Allium tuberosum Rottler) Depending on Retail Types

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