Bactericidal/permeability-increasing protein in the reproductive system of male mice may be involved in the sperm–oocyte fusion

Li, Kun; Liu, Yue; Xia, Xiaoyu; Wang, Li; Lu, Meige; Hu, Yanqin; Xu, Chen

doi:10.1530/rep-13-0127

Cited by 11 publications

(5 citation statements)

References 42 publications

(36 reference statements)

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“…To confirm that GSK3 did indeed reside within dense bodies, epididymal sections were dual‐labeled with combinations of anti‐GSK3, anti‐HSPD1, and anti‐BPI antibodies, the latter being established markers of the dense body population (27, 36). All proteins strongly colocalized within the majority of dense bodies (Fig.…”

Section: Resultsmentioning

confidence: 99%

Glycogen synthase kinase 3 regulates acrosomal exocytosis in mouse spermatozoa via dynamin phosphorylation

et al. 2015

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The dynamin family of GTPases has been implicated as novel regulators of the acrosome reaction, a unique exocytotic event that is essential for fertilization. Dynamin activity during the acrosome reaction is accompanied by phosphorylation of key serine residues. We now tested the hypothesis that glycogen synthase kinase 3 (GSK3) is the protein kinase responsible for dynamin phosphorylation at these phosphosites in mouse spermatozoa. Pharmacologic inhibition of GSK3 in mature mouse spermatozoa (CHIR99021: IC50 = 6.7 nM) led to a significant reduction in dynamin phosphorylation (10.3% vs. 27.3%; P < 0.001), acrosomal exocytosis (9.7% vs. 25.7%; P < 0.01), and in vitro fertilization (53% vs. 100%; P < 0.01). GSK3 was shown to be present in developing germ cells where it colocalized with dynamin in the peri-acrosomal domain. However, additional GSK3 was acquired by maturing mouse spermatozoa within the male reproductive tract, via a novel mechanism involving direct interaction of sperm heads with extracellular structures known as epididymal dense bodies. These data reveal a novel mode for the cellular acquisition of a protein kinase and identify a key role for GSK3 in the regulation of sperm maturation and acrosomal exocytosis.

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Section: Resultsmentioning

confidence: 99%

Glycogen synthase kinase 3 regulates acrosomal exocytosis in mouse spermatozoa via dynamin phosphorylation

et al. 2015

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“…In this regard, although the function for BPI in FF is still unknown, the presence of BPIFA2 during the ovulatory seasons may be important to protect the female genital tract (e.g., oviduct). Also, few reports have found that BPI is expressed in the testis and epididymis of mice and appears to take part in the process of gamete interactions [42, 43].…”

Section: Discussionmentioning

confidence: 99%

Seasonal variation in equine follicular fluid proteome

Dutra

Ishak

Pecháňová

et al. 2019

Reprod Biol Endocrinol

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Background Proteomic studies of follicular fluid (FF) exist for several species, including the horse; however, the seasonal influence on FF proteome has not been explored in livestock. The application of high-throughput proteomics of FF in horse has the potential to identify seasonal variations of proteins involved in follicle and oocyte growth. Methods This study (i) profiles the proteomes of equine FF collected from dominant growing follicles during the spring anovulatory season (SAN), and spring (SOV), summer (SUM), and fall (FOV) ovulatory seasons; and (ii) identifies season-dependent regulatory networks and associated key proteins. Results Regardless of season, a total of 90 proteins were identified in FF, corresponding to 63, 72, 69, and 78 proteins detected in the SAN, SOV, SUM, and FOV seasons, respectively. Fifty-two proteins were common to all seasons, a total of 13 were unique to either season, and 25 were shared between two seasons or more. Protein-to-protein interaction (PPI) analysis indicated the likely critical roles of plasminogen in the SAN season, the prothrombin/plasminogen combination in SUM, and plasminogen/complement C3 in both SOV and FOV seasons. The apolipoprotein A1 appeared crucial in all seasons. The present findings show that FF proteome of SUM differs from other seasons, with FF having high fluidity (low viscosity). Conclusions The balance between the FF contents in prothrombin, plasminogen, and coagulation factor XII proteins favoring FF fluidity may be crucial at the peak of the ovulatory season (SUM) and may explain the reported lower incidence of hemorrhagic anovulatory follicles during the SUM season. Electronic supplementary material The online version of this article (10.1186/s12958-019-0473-z) contains supplementary material, which is available to authorized users.

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“…In the male mice, BPI is speci cally expressed in the testis and epididymis (Lennartsson et al 2005). After secretion from the epididymal epithelia, BPI is transferred to membrane surface of the spermatozoa (Zhou et al 2014), and is essential for the sperm-oocyte fusion (Li et al 2013, Yano et al 2010. MPO serves as peroxidase lysosomal enzyme that produces hypochlorous acid and other highly reactive oxidants (Klebanoff et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Genes and Pathways Underpinning Klinefelter Syndrome at Bulk and Single-Cell Levels

Tian,

Yu,

Mao

et al. 2024

Biochem Genet

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Klinefelter syndrome (KS) is the most frequent genetic anomaly in infertile males. Despite this, the molecular mechanisms involved in KS are poorly de ned. Based on bulk transcriptome and single-cell RNA sequencing datasets with peripheral blood monocyte (PBMC) sample(s) from healthy and KS men, this study was designed to address critical genes and pathways correlated with the occurrence of KS.Through a comparison between control and KS samples, we obtained 5 hub genes, including two upregulated genes (XG and ITLN1) and three downregulated genes (DEFA4, BPI and MPO). Without exception, these ve genes yielded an excellent discriminatory capacity for KS with an area under the receiver-operator-characteristic curve over 0.75. We also assessed between-group differences of immune cell in ltration using ssGSEA. In ltrated degree of some immune cells such as CD56 bright NK cell was found to be positively associated with the expression of ITLN1 and XG. Through Kyoto Encyclopedia of Genes and Genomes enrichment, we identi ed PI3K/AKT pathway and neuroactive ligand-receptor interaction as upregulated pathways for KS. Gene set enrichment analysis together with gene set variation analysis con rmed upregulation of G2M checkpoint, mitotic spindle, and heme metabolism for KS. Furthermore, scRNA-seq data analysis was conducted to detect intercellular communication between different immune cell types, and a strong correlation was detected for macrophages, dendritic cells or NK cells with the other cell types. Collectively, we provided hub genes, pathways, immune cell in ltration degree, and cell-cell communication interactions for KS, warranting novel insights into the mechanisms of KS.

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Bactericidal/permeability-increasing protein in the reproductive system of male mice may be involved in the sperm–oocyte fusion

Cited by 11 publications

References 42 publications

Glycogen synthase kinase 3 regulates acrosomal exocytosis in mouse spermatozoa via dynamin phosphorylation

Glycogen synthase kinase 3 regulates acrosomal exocytosis in mouse spermatozoa via dynamin phosphorylation

Seasonal variation in equine follicular fluid proteome

Genes and Pathways Underpinning Klinefelter Syndrome at Bulk and Single-Cell Levels

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