Bacteriobiota of the Cave Church of Sts. Peter and Paul in Serbia—Culturable and Non-Culturable Communities’ Assessment in the Bioconservation Potential of a Peculiar Fresco Painting

Dimkić, Ivica; Ćopić, Milica; Petrović, Marija; Stupar, M.; Savković, Željko; Knežević, Aleksandar; Simić, Gordana Subakov; Grbić, Milica Ljaljević; Unković, Nikola

doi:10.3390/ijms24021016

Cited by 8 publications

(3 citation statements)

References 99 publications

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“…Methylocella, Methylovirgula and Mycobacterium) strains were promoted by warmer and humid climatic conditions (lower MDR/TSEA and/or higher AI/PSEA/MAP). Conversely, some phototrophic (Cyanobacteria and Roseiflexus) and rock weathering (Rubrobacter and Actinomycetospora) bacterial genera were positively associated with higher arid conditions (Saiz-Jimenez, 2012;Farda et al, 2022;Dimkić et al, 2023). In light of this, we would propose that climatic variation represents one of the main epistatic drivers in determining the energetic status of caves and the assembling of microbial community cores.…”

Section: Discussionmentioning

confidence: 93%

Outdoor Climate Drives Diversity Patterns of Dominant Microbial Taxa in Caves Worldwide

Biagioli,

Coleine,

Delgado-Baquerizo

et al. 2023

Preprint

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Section: Discussionmentioning

confidence: 93%

Outdoor Climate Drives Diversity Patterns of Dominant Microbial Taxa in Caves Worldwide

Biagioli,

Coleine,

Delgado-Baquerizo

et al. 2023

Preprint

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“…This order has previously been identified in samples obtained from a fresco painting inside the Cave Church of the Sts. Peter and Paul [48]. In addition, it has been observed that bacteria of this order, which are capable of withstanding stressful conditions, have demonstrated improved functional pathways related to the metabolism of amino acids and carbohydrates [49].…”

Section: Description Of the Biodiversity Of Prokaryotic Microorganism...mentioning

confidence: 99%

16S and 18S rDNA Amplicon Sequencing Analysis of Aesthetically Problematic Microbial Mats on the Walls of the Petralona Cave: The Use of Essential Oils as a Cleaning Method

Tsouggou,

Oikonomou,

Papadimitriou

et al. 2023

Microorganisms

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The presence of microbial communities on cave walls and speleothems is an issue that requires attention. Traditional cleaning methods using water, brushes, and steam can spread the infection and cause damage to the cave structures, while chemical agents can lead to the formation of toxic compounds and damage the cave walls. Essential oils (EOs) have shown promising results in disrupting the cell membrane of bacteria and affecting their membrane permeability. In this study, we identified the microorganisms forming unwanted microbial communities on the walls and speleothems of Petralona Cave using 16S and 18S rDNA amplicon sequencing approaches and evaluated the efficacy of EOs in reducing the ATP levels of these ecosystems. The samples exhibited a variety of both prokaryotic and eukaryotic microorganisms, including Proteobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Firmicutes, the SAR supergroup, Opisthokonta, Excavata, Archaeplastida, and Amoebozoa. These phyla are often found in various habitats, including caves, and contribute to the ecological intricacy of cave ecosystems. In terms of the order and genus taxonomy, the identified biota showed abundances that varied significantly among the samples. Functional predictions were also conducted to estimate the differences in expressed genes among the samples. Oregano EO was found to reduce ATP levels by 87% and 46% for black and green spots, respectively. Consecutive spraying with cinnamon EO further reduced ATP levels, with reductions of 89% for black and 88% for green spots. The application of a mixture solution caused a significant reduction up to 96% in ATP levels of both areas. Our results indicate that EOs could be a promising solution for the treatment of microbial communities on cave walls and speleothems.

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“…and Achromobacter xylosoxidans NUU2, where B. subtilis NUU4 had signi cant plant growth promotion capabilities, improved rhizobia symbiosis, and reduced the infection rate of root rot caused by F. solani. It is a biocontrol strategy in which the control agent consumes nutritional resources before the pathogen, which is advantageous when the fungus grows quickly and covers a large area; nutrient and space competition is one proposed inhibition mechanism for Aspergillus species suppression(Lahlali et al 2022;Dimkić et al 2023). Comparably, Sharma et al 2021 studied the diversity pattern and fungicidal activity of bacterial endophytes isolated from two different organic tomato varieties (V1 and V2).…”

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confidence: 99%

Promoting the growth of Sulla flexuosa L. by endophytic root nodule bacteria authors and affiliations

Hamane

Yemlahi

Zerrouk

et al. 2023

World J Microbiol Biotechnol

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Legume plants rely upon multipartite interactions between rhizobia and bacterial endophytes within root nodules to facilitate plant growth. This study sought to isolate and identify indigenous endophytic bacteria from root nodules of Sulla aculeolata L. in Northeast Morocco. Based on their tri-calcium phosphate (TCP) solubilization capacity, ve endophytes were chosen for further evaluation of their plant growth activities. All isolates were hydrogen cyanide (HCN) and siderophore producers, while only BCH24 tested positive for ACC deaminase activity. Indole-3-acetic acid (IAA) synthesis ranged from 1.27 mg L − 1 to 2.89 mg L − 1 , with soluble phosphate concentrations between 7.99 mg L − 1 and 110.58 mg L − 1 . Additionally, all the endophytes were able to produce more than two lytic enzymes. The 16S rRNA gene sequencing identi ed the ve isolates as Enterobacter sp (BCH13, BCH2), Pseudomonas sp (BCH16, BCH24), and Serratia sp (BCH10). The strains inhibited the growth of three phytopathogenic fungi, with BCH13 exhibiting the highest rate against Aspergillus ochraceus (45%), followed by BCH24 against Fusarium oxysporum (40%) and Botrytis cinerea (35%), respectively. In vivo inoculation of halotolerant strains Enterobacter hormaechei (BCH13) and Pseudomonas moraviensis (BCH16) under chamber conditions revealed that co-inoculation with Rhizobium sullae KS6 improved plant development compared to single inoculation, making it a promising eco-friendly bio-inoculant for legume Sulla exuosa L. production.

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Bacteriobiota of the Cave Church of Sts. Peter and Paul in Serbia—Culturable and Non-Culturable Communities’ Assessment in the Bioconservation Potential of a Peculiar Fresco Painting

Cited by 8 publications

References 99 publications

Outdoor Climate Drives Diversity Patterns of Dominant Microbial Taxa in Caves Worldwide

Outdoor Climate Drives Diversity Patterns of Dominant Microbial Taxa in Caves Worldwide

16S and 18S rDNA Amplicon Sequencing Analysis of Aesthetically Problematic Microbial Mats on the Walls of the Petralona Cave: The Use of Essential Oils as a Cleaning Method

Promoting the growth of Sulla flexuosa L. by endophytic root nodule bacteria authors and affiliations

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