Bacteriocin producing Enterococcus faecalis isolated from chicken gastrointestinal tract originating from Phitsanulok, Thailand: Isolation, screening, safety evaluation and probiotic properties

Hwanhlem, Noraphat; Ivanova, Teodora; Bíscola, Vanessa; Choiset, Yvan; Haertlé, Thomas

doi:10.1016/j.foodcont.2017.02.060

Cited by 38 publications

(22 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…including 4 E. faecalis , 7 E. faecium and 2 E. durans . In general, our tolerance results to gastrointestinal conditions are similar with those reported by El-Jeni et al [12] and Hwanhlem et al [13].…”

Section: Resultssupporting

confidence: 92%

Isolation, identification, and potential probiotic characterization of isolated lactic acid bacteria and in vitro investigation of the cytotoxicity, antioxidant, and antidiabetic activities in fermented sausage

2019

View full text Add to dashboard Cite

BackgroundProbiotic bacteria can provide health benefits when delivered in functional foods. This study involved isolation of lactic acid bacteria (LAB) from traditionally dried and salted anchovy fish and characterization of their survival in simulated gastrointestinal digestion. Promising strains were used to prepare fermented fish sausages which were then evaluated for cytotoxicity activity against two cancer cell-lines, antidiabetic activity as determined by α-amylase and α-glucosidase inhibition, and antioxidant and proteolytic activities in vitro, as compared to non-fermented control sausages.ResultsOut of 85 LAB obtained, 13 isolates with high tolerance to simulated gastrointestinal digestion were obtained, which were identified as Enterococcus spp. Four E. faecium strains, one E. faecalis, and one E. durans were used separately to make fermented fish sausages. The α-amylase and α-glucosidase inhibition from fish sausages fermented by Enterococcus spp. ranged from 29.2 to 68.7% and 23.9 to 41.4%, respectively, during 21 days of storage. The cytotoxicity activities against Caco2 and MCF-7 cells of fish sausages fermented with Enterococcus spp. ranged from 18.0 to 24% and 13.9 to 27.9%, respectively. Cytotoxicity activities correlated positively with proteolysis and antioxidant activities, α-amylase and α-glucosidase inhibition activities, but negatively with the pH in fermented fish sausages. Strains also exhibited antimicrobial activity against foodborne pathogens and presented no significant concerns with regards to antibiotic resistance or virulence gene content.ConclusionsFish sausages fermented by potential probiotic isolates of Enterococcus spp. from dried fish had valuable health-promoting benefits compared with non-fermented control sausages.

show abstract

Section: Resultssupporting

confidence: 92%

Isolation, identification, and potential probiotic characterization of isolated lactic acid bacteria and in vitro investigation of the cytotoxicity, antioxidant, and antidiabetic activities in fermented sausage

2019

View full text Add to dashboard Cite

show abstract

“…It can effectively inhibit the spoilage of food spoilage bacteria and a variety of pathogenic microorganisms (Goyal, Malik, & Pradhan, 2018;Kassaa et al, 2019;Mokoena, 2017), and bacteria were more immune to the produced bacteriocin (Milioni et al, 2015). It can be developed as a new preservative due to its natural nontoxicity and good selectivity (Hwanhlem, Ivanova, Biscola, Choiset, & Haertlé, 2017;Zhao et al, 2016). Nisin was the most representative LAB bacteriocin recognized by the public as a safe and harmless food additive (Ge, Teng, Wang, & Zhao,2016), but it had many shortcomings, for example, it had a narrow spectrum of inhibition which could be inhibited most Gram-positive bacteria, but had no significantly affected on Gram-negative bacteria, yeast, and mold.…”

Section: Introductionmentioning

confidence: 99%

Partial purification and characterization of a broad‐spectrum bacteriocin produced by a Lactobacillus plantarum zrx03 isolated from infant's feces

Lei

Zhao

Sun

et al. 2020

Food Science & Nutrition

View full text Add to dashboard Cite

Lactobacillus plantarum zrx03 was a bacteriocin-producing strain isolated from infant's feces. The fermentation supernatant produced by this strain could strongly inhibit Escherichia coli JM109 ATCC 67387, Staphylococcus aureus ATCC 25923, and Listeria monocytogenes CICC 21633, in which the diameter of inhibition zone was 12.83 ± 0.62 mm, 15.08 ± 0.31 mm, 6.75 ± 0.20 mm, respectively, compared with lactic acid bacteria N1, N2, M13, M21, M31, and M37. According to amplification of 16S rRNA gene and identification of phylogenetic tree, this strain had a 1,450 bp sequence and 100% identity to the L. plantarum strain. Based on the influence of different protease treatments, such as pepsin, trypsin, papain, and proteinase K on the antimicrobial activity, this antimicrobial substance was considered to be a natural protein. Using bacteriocin produced by this strain as study object of this experiment, it had been extracted from ammonium sulfate precipitation and different organic solvents. The results showed that ethyl acetate was selected as the optimal solution to crude extraction of bacteriocin after comparing ammonium sulfate precipitation method and organic solvent extraction method, such as n-butanol, n-hexane, dichloromethane, trichloromethane, in which the diameter of the inhibition zones was above 28 mm. Results also showed the inhibition spectrum of the obtained bacteriocin had a broad spectrum of inhibition which could inhibit Gram-positive, Gramnegative, yeast. Especially, it could effectively inhibit S. aureus ATCC 25923, Bacillus subtilis CICC 10002, Bacillus anthracis CICC 20443, E. coli JM109 ATCC 67387, and Salmonella CMCC 541, and the zone diameter of inhibition has reached more than 28 mm. Moreover, it had a good thermal stability which antibacterial activity was retained 70.58% after treatment at 121°C for 30 min, and pH-stability was between pH 2.0-9.0. These results suggested bacteriocin produced by L. plantarum zrx03 had potential application prospects in food preservation. K E Y W O R D Santimicrobial stability, bacteriocin, Lactobacillus plantarum zrx03, purification | 2215 LEI Et aL.

show abstract

“…Genomic DNA was used as the template (16S rDNA) with universal primers fD1 ( ) and rD1 ( ). The PCR conditions included a first step of 95°C for 2 minutes and 40 cycles of 95°C for 30 seconds (denaturation), followed by 45°C for 30 seconds (annealing) and 72°C for 30 seconds (extension) with a final extension step of 72°C for 7 minutes [ 18 ]. Amplicon was confirmed by gel-electrophoresis for 45 minutes at 60 V in 1% (w/v) agarose gel with ethidium bromide (0.5 mg/ml) in 0.5TAE (Tris-Acetate-EDTA) buffer (pH 8.0) and then observed under UV light.…”

Section: Methodsmentioning

confidence: 99%

Integrated genome-based probiotic relevance and safety evaluation of Lactobacillus reuteri PNW1

et al. 2020

View full text Add to dashboard Cite

This study evaluates whole-genome sequence of Lactobacillus reuteri PNW1 and identifies its safety genes that may qualify it as a putative probiotic. It further extracted the bacteriocin produced by the strain and tested its effectiveness against pathogenic STEC E. coli O177. The genomic DNA was sequenced on illuminal Miseq instrument and the sequenced data was assessed for quality reads before assembled with SPAdes. The draft assembly was annotated with Prokaryotic Genome Annotation Pipeline (PGAP) and Rapid Annotations using Subsystems Technology (RAST). Further downstream analyses were carried out using appropriate bioinformatic tools. Production of biogenic amines was biochemically confirmed through HPLC analysis. The assembled genome was 2,430,215 bp long in 420 contigs with 39% G+C content. Among all known genes, putatively responsible for the production of toxic biochemicals, only arginine deiminase (EC3.5.3.6) was spotted. Coding sequences (CDS) putative for D-lactate dehydrogenase (EC1.1.1.28), L-lactate dehydrogenase (EC1.1.1.27) and bacteriocin helveticin J were found within the genome together with plethora of other probiotic important genes. The strain harbours only resistant genes putative for Lincosamide (lnuC) and Tetracycline resistant genes (tetW). There was no hit found for virulence factors and probability of the strain being a human pathogen was zero. Two intact prophage regions were detected within the genome of L. reuteri PNW1 and nine CDS were identified for insertion sequence by OASIS which are belong to seven different families. Five putative CDS were identified for the CRISPR, each associated with Cas genes. Maximum zone of inhibition exhibited by the bacteriocin produced L. reuteri PNW1 is 20.0 ±1.00 mm (crude) and 23.3±1.15 mm (at 0.25 mg/ml) after being partially purified. With the strain predicted as non-human pathogen, coupled with many other identified desired features, L. reuteri PNW1 stands a chance of making good and safe candidates for probiotic, though further in-vivo investigations are still necessary.

show abstract

Bacteriocin producing Enterococcus faecalis isolated from chicken gastrointestinal tract originating from Phitsanulok, Thailand: Isolation, screening, safety evaluation and probiotic properties

Cited by 38 publications

References 62 publications

Isolation, identification, and potential probiotic characterization of isolated lactic acid bacteria and in vitro investigation of the cytotoxicity, antioxidant, and antidiabetic activities in fermented sausage

Isolation, identification, and potential probiotic characterization of isolated lactic acid bacteria and in vitro investigation of the cytotoxicity, antioxidant, and antidiabetic activities in fermented sausage

Partial purification and characterization of a broad‐spectrum bacteriocin produced by a Lactobacillus plantarum zrx03 isolated from infant's feces

Integrated genome-based probiotic relevance and safety evaluation of Lactobacillus reuteri PNW1

Contact Info

Product

Resources

About