Bacteriocin production: a relatively unharnessed probiotic trait?

Hegarty, James W.; Guinane, Caitriona M.; Ross, R. Paul; Hill, Colin; Cotter, Paul D.

doi:10.12688/f1000research.9615.1

Cited by 121 publications

(91 citation statements)

References 75 publications

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“…While their potential in applications has so far mostly been seen in the areas of preservatives and probiotics [3], they have been of increasing interest since novel therapeutic applications have been discovered [4][5][6]. Lantibiotics are gene-encoded and the genes involved in their biosynthesis, regulation and immunity are usually clustered.…”

Section: Introductionmentioning

confidence: 99%

Discovery of a novel lantibiotic nisin O from Blautia obeum A2-162, isolated from the human gastrointestinal tract

et al. 2017

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A novel lanC-like sequence was identified from the dominant human gut bacterium Blautia obeum strain A2-162. This sequence was extended to reveal a putative lantibiotic operon with biosynthetic and transport genes, two sets of regulatory genes, immunity genes, three identical copies of a nisin-like lanA gene with an unusual leader peptide, and a fourth putative lanA gene. Comparison with other nisin clusters showed that the closest relationship was to nisin U. B. obeum A2-162 demonstrated antimicrobial activity against Clostridium perfringens when grown on solid medium in the presence of trypsin. Fusions of predicted nsoA structural sequences with the nisin A leader were expressed in Lactococcus lactis containing the nisin A operon without nisA. Expression of the nisA leader sequence fused to the predicted structural nsoA1 produced a growth defect in L. lactis that was dependent upon the presence of biosynthetic genes, but failed to produce antimicrobial activity. Insertion of the nso cluster into L. lactis MG1614 gave an increased immunity to nisin A, but this was not replicated by the expression of nsoI. Nisin A induction of L. lactis containing the nso cluster and nisRK genes allowed detection of the NsoA1 pre-peptide by Western hybridization. When this heterologous producer was grown with nisin induction on solid medium, antimicrobial activity was demonstrated in the presence of trypsin against C. perfringens, Clostridium difficile and L. lactis. This research adds to evidence that lantibiotic production may be an important trait of gut bacteria and could lead to the development of novel treatments for intestinal diseases.

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Section: Introductionmentioning

confidence: 99%

Discovery of a novel lantibiotic nisin O from Blautia obeum A2-162, isolated from the human gastrointestinal tract

et al. 2017

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“…The production of this type of compounds is related to environmental conditions such as pH, temperature, nutrients, and competitiveness . Antimicrobial compounds producers LAL can survive in the gastrointestinal tract due to the direct growth inhibition potential of bacteriocin toward other microorganisms (especially pathogens) . Natural antimicrobial compounds (mainly bacteriocins) have also been receiving attention due to their use potential in the food industry as natural preservatives …”

Section: Production Of Antimicrobial Compounds By Lalmentioning

confidence: 99%

“…83 Antimicrobial compounds producers LAL can survive in the gastrointestinal tract due to the direct growth inhibition potential of bacteriocin toward other microorganisms (especially pathogens). 84,85 Natural antimicrobial compounds (mainly bacteriocins) have also been receiving attention due to their use potential in the food industry as natural preservatives. 86 As reviewed by Sabo et al, 87 (rha).…”

Section: Production Of Antimicrobial Compounds By Lalmentioning

confidence: 99%

Acid lactic lactobacilli as a biotechnological toll to improve food quality and human health

Sousa

Rama

Souza

et al. 2019

Biotechnology Progress

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The genus Lactobacillus has been widely used in food industry as starter or adjunct culture due to its probiotic features. Its biotechnological features improve the spectrum of uses of lactobacilli, which can affect its applicability directly. In this sense, this literature review gathers information and discusses the biotechnological potential of technological/probiotic lactobacilli aiming to improve food quality and human health. The primary and secondary metabolism generates specific substances, such as organic acids, carbon dioxide, hydrogen peroxide, diacetyl, fatty acids, and bacteriocins, which are determinant due to their probiotic potential, antimicrobial activity, and the development of new food flavors. In order to become industrially and commercially attractive, it is necessary develop a large‐scale process with lower production costs.

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“…51 Importantly, this work supports the idea that bacteriocins likely represent an important cog in the probiotic machinery that dictates, to a large extent, human health. 72 …”

Section: Animal-microbe Symbiosesmentioning

confidence: 99%

Symbiosis-inspired approaches to antibiotic discovery

2017

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Life on Earth is characterized by a remarkable abundance of symbiotic and highly refined relationships among life forms. Defined as any kind of close, long-term association between two organisms, symbioses can be mutualistic, commensalistic or parasitic. Historically speaking, selective pressures have shaped symbioses in which one organism (typically a bacterium or fungus) generates bioactive small molecules that impact the host (and possibly other symbionts); the symbiosis is driven fundamentally by the genetic machineries available to the small molecule producer. The human microbiome is now integral to the most recent chapter in animal-microbe symbiosis studies and plant-microbe symbioses have significantly advanced our understanding of natural products biosynthesis; this also is the case for studies of fungal-microbe symbioses. However, much less is known about microbe-microbe systems involving interspecies interactions. Microbe-derived small molecules (i.e. antibiotics and quorum sensing molecules, etc.) have been shown to regulate transcription in microbes within the same environmental niche, suggesting interspecies interactions whereas, intraspecies interactions, such as those that exploit autoinducing small molecules, also modulate gene expression based on environmental cues. We, and others, contend that symbioses provide almost unlimited opportunities for the discovery of new bioactive compounds whose activities and applications have been evolutionarily optimized. Particularly intriguing is the possibility that environmental effectors can guide laboratory expression of secondary metabolites from “orphan”, or silent, biosynthetic gene clusters (BGCs). Notably, many of the studies summarized here result from advances in “omics” technologies and highlight how symbioses have given rise to new anti-bacterial and antifungal natural products now being discovered.

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Bacteriocin production: a relatively unharnessed probiotic trait?

Cited by 121 publications

References 75 publications

Discovery of a novel lantibiotic nisin O from Blautia obeum A2-162, isolated from the human gastrointestinal tract

Discovery of a novel lantibiotic nisin O from Blautia obeum A2-162, isolated from the human gastrointestinal tract

Acid lactic lactobacilli as a biotechnological toll to improve food quality and human health

Symbiosis-inspired approaches to antibiotic discovery

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