Bacteriocin production by Clostridium acetobutylicum in an industrial fermentation process

Abstract

High titers of a noninducible bacteriocin were produced by Clostridium acetobutylicum in a molasses fermentation medium used for the industrial production of solvents. Release of the bacteriocin towards the end of the exponential growth phase was accompanied by lysis of the culture and inhibition of the production of solvents. The producer cells were sensitive to the bacteriocin, which only affected other C. acetobutylicum strains and a Clostridium felsineum strain. The thermolabile bacteriocin was not inactivated by protease enyzmes and had an optimum stability between pH 4 and 5. The sedimentation coefficient of the bacteriocin was 6S.

Butanol Production by a Butanol-Tolerant Strain of Clostridium acetobutylicum in Extruded Corn Broth

“…The acetone-butanol-ethanol fermentation with Clostridium acetobutylicum has a history of using primarily molasses or corn as carbohydrate source. Physiological determinations associated with solvent production have been carried out with corn mash (19) and, more recently, molasses (3,15). In the midwest, interest in butanol production from corn has been reinitiated due to the periodic abundance of mycotoxin-contaminated waste corn and a shortage of energy on the farm.…”

Purification and Characterization of an Autolysin from Clostridium acetobutylicum

“…Bacterial strains and media. The C. acetobutylicum strains and the bacterial strains used in the activity spectrum studies (Table 1) were described previously (1). The potato medium used for the maintenance of the C. acetobutylicum strain and the molasses fermentation medium (CFM) used for the production of the autolysin were described by Barber et al (1).…”

Structure and transcription of genes within the β-hbd-adh1 region of Clostridium acetobutylicum P262

“…The C, acetobutylicum P262 cells were grown in batch culture in CBM and mRNA was isolated at 8, 12, 16 and 24 h. The growth of the C. acetobutylicum cells was monitored and at each of the sampling times the cells were examined microscopically and the pH and presence of acids and solvents were determined by gas chromatography as described by Barber et al [14] (Fig. 3).…”