Bacteriocin production by Enterococcus faecium FAIR-E 198 in view of its application as adjunct starter in Greek Feta cheese making

Sarantinopoulos, Panagiotis; Leroy, Frédéric; Leontopoulou, E.; Georgalaki, Marina; Kalantzopoulos, George; Tsakalidou, Effie; Vuyst, Luc De

doi:10.1016/s0168-1605(01)00633-x

Cited by 96 publications

(52 citation statements)

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“…Enterocins are active against many Gram-positive bacteria encompassing undesirable and pathogenic microbes, such as Clostridia, Bacillus, Staphylococcus and Listeria, as well as some Gram-negative bacteria [22,23,43]. There are several reports regarding the application of bacteriocin-producing enterococci as starter or protective cultures or use of their bacteriocins for protection against spoilage and pathogenic bacteria in cheese [21,28,30,50]. This fact stimulates the special interest in enterocins as potential food preservatives.…”

Section: Introductionmentioning

confidence: 99%

Incidence of virulence in bacteriocin-producing enterococcal isolates

Gupta

Malik

2007

Lait

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-Enterococci isolated from a total number of 68 dairy products and 28 human fecal samples were screened for bacteriocin production. The incidence of the bacteriocin-production trait was highest among the cream samples (33.51%), followed by dahi samples (15.55%), raw milk samples (9.51%), Cheddar cheese (9.2%) samples and human feces (4.72%). The isolates showed antilisterial effect besides activity against other lactic acid bacteria. Sixty potent bacteriocin-producing isolates of enterococci were evaluated for the incidence of virulence characters for their safe exploitation. None of the isolates was found to exhibit gelatinase, DNAse or β-hemolysis activity. Furthermore, only 4 bacteriocin-producing isolates among 60 were observed to be vancomycinresistant. It was concluded that bacteriocin-producing Enterococcus strains lacking hemolytic activity and not carrying vancomycin resistance genes may be safely exploited as starter cultures, co-cultures or probiotics. Résumé -Incidence de la virulence d'isolats d'entérocoques produisant une bactériocine.Des entérocoques isolés de 68 produits laitiers et 28 échantillons fécaux humains ont été criblés pour leur production de bactériocine. L'incidence de ce caractère était maximale dans les échantillons de crème (33,51 %), suivis des échantillons de dahi (15,55 %), de lait cru (9,51 %), fèces humains (4,72 %) et fromage Cheddar (9,2 %). Outre leur activité contre les autres bactéries lactiques, les isolats avaient un effet anti-listeria. Soixante isolats d'entérocoques ayant la capacité de production de bactériocine ont été évalués pour l'incidence de leurs caractères de virulence sur leur sécurité d'emploi. Aucun des isolats n'a montré d'activité gélatinase, DNAse et β-hémolyse.

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Section: Introductionmentioning

confidence: 99%

Incidence of virulence in bacteriocin-producing enterococcal isolates

Gupta

Malik

2007

Lait

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“…Application of enterococcal bacteriocins on dairy foods has been the focus of many investigations (Foulique Moreno et al 2006;Giraffa 1995). Enterococcus strains displaying a limited inhibitory spectrum due to the production of enterocins targeted towards Listeria and/or Clostridium (Franz et al 1996;Giraffa 1995;Torri Tarelli et al 1994) would be interesting as protective cultures for cheese manufacture, given their very limited antagonistic activity towards dairy starter cultures such as Lactococcus and Streptococcus (Foulique Moreno et al 2006;Sarantinopoulos et al 2002). Recently, Lactobacillus brevisFPTLB3 isolated from freshwater fish has been reported to produce bacteriocin that had broad spectrum of inhibition (3200 AU/ml) againstEscherichia coli MTCC 1563, Enterococcus faecalis MTCC 2729, Lactobacillus casei MTCC 1423, Lactobacillus sakei ATCC 15521 and Staphylococcus aureus ATCC 25923 (Banerjee et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial efficacy of nisin, pediocin 34 and enterocin FH99 against L. monocytogenes, E. faecium and E. faecalis and bacteriocin cross resistance and antibiotic susceptibility of their bacteriocin resistant variants

et al. 2011

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The bacteriocin susceptibility of Listeria monocytogenes MTCC 657, Enterococcus faecium DSMZ 20477, E. faecium VRE, and E. faecalis ATCC 29212 and their corresponding bacteriocin resistant variants was assessed. The single and combined effect of nisin and pediocin 34 and enterocin FH99 bacteriocins produced by Pediococcus pentosaceus 34, and E. faecium FH99, respectively, was determined. Pediocin34 proved to be more effective in inhibiting L. monocytogenes MTCC 657. A greater antibacterial effect was observed against E. faecium DSMZ 20477 and E. faecium (VRE) when the a combination of nisin, pediocin 34 and enterocin FH99 were used whereas in case of L. monocytogenes MTCC 657 a combination of pediocin 34 and enterocin FH99 was more effective in reducing the survival of pathogen. Bacteriocin cross-resistance and the antibiotic susceptibility of wild type and their corresponding resistant variants were assessed and results showed that resistance to a bacteriocin may extend to other bacteriocins within the same class and also the acquired resistance to bacteriocins can modify the antibiotic susceptibility/resistance profile of the bacterial species used in the study. According to the hydrophobicity nisin resistant variant of L. monocytogenes was more hydrophobic (p<0.001), whereas the pediocin 34 and enterocin FH99 resistant variants were less hydrophobic than the wild type strain. Nisin, pediocin 34 and enterocin FH99 resistant variants of E. faecium DSMZ 20477 and E. faecium VRE were less hydrophobic than their wild type counterparts. Nisin resistant E. faecalis ATCC 29212 was less hydrophobic than its wild type counterpart.

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“…Therefore, these multiple-bacteriocin-producing LAB strains are regarded as potentially useful in the control of various bacteria. For instance, they are considered to be useful antimicrobial starter cultures for food preservation (28)(29)(30). However, in multiple bacteriocins, biosynthetic mechanisms such as secretion and immunity have not been deeply understood and the information is quite limited.…”

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confidence: 99%

Gene Cluster Responsible for Secretion of and Immunity to Multiple Bacteriocins, the NKR-5-3 Enterocins

Ishibashi

Himeno

Masuda

et al. 2014

Appl Environ Microbiol

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dEnterococcus faecium NKR-5-3, isolated from Thai fermented fish, is characterized by the unique ability to produce five bacteriocins, namely, enterocins NKR-5-3A, -B, -C, -D, and -Z (Ent53A, Ent53B, Ent53C, Ent53D, and Ent53Z). Genetic analysis with a genome library revealed that the bacteriocin structural genes (enkA [ent53A], enkC [ent53C], enkD [ent53D], and enkZ [ent53Z]) that encode these peptides (except for Ent53B) are located in close proximity to each other. This NKR-5-3ACDZ (Ent53ACDZ) enterocin gene cluster (approximately 13 kb long) includes certain bacteriocin biosynthetic genes such as an ABC transporter gene (enkT), two immunity genes (enkIaz and enkIc), a response regulator (enkR), and a histidine protein kinase (enkK). Heterologous-expression studies of enkT and ⌬enkT mutant strains showed that enkT is responsible for the secretion of Ent53A, Ent53C, Ent53D, and Ent53Z, suggesting that EnkT is a wide-range ABC transporter that contributes to the effective production of these bacteriocins. In addition, EnkIaz and EnkIc were found to confer self-immunity to the respective bacteriocins. Furthermore, bacteriocin induction assays performed with the ⌬enkRK mutant strain showed that EnkR and EnkK are regulatory proteins responsible for bacteriocin production and that, together with Ent53D, they constitute a three-component regulatory system. Thus, the Ent53ACDZ gene cluster is essential for the biosynthesis and regulation of NKR-5-3 enterocins, and this is, to our knowledge, the first report that demonstrates the secretion of multiple bacteriocins by an ABC transporter.

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Bacteriocin production by Enterococcus faecium FAIR-E 198 in view of its application as adjunct starter in Greek Feta cheese making

Cited by 96 publications

References 54 publications

Incidence of virulence in bacteriocin-producing enterococcal isolates

Incidence of virulence in bacteriocin-producing enterococcal isolates

Antibacterial efficacy of nisin, pediocin 34 and enterocin FH99 against L. monocytogenes, E. faecium and E. faecalis and bacteriocin cross resistance and antibiotic susceptibility of their bacteriocin resistant variants

Gene Cluster Responsible for Secretion of and Immunity to Multiple Bacteriocins, the NKR-5-3 Enterocins

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