Bacteriocin production by lactic acid bacteria isolated from fish, seafood and fish products

Gómez‐Sala, Beatriz; Muñoz-Atienza, Estefanía; Sánchez, Jorge; Basanta, Antonio; Herranz, Carmen; Hernández, Pablo E.; Cintas, Luis M.

doi:10.1007/s00217-015-2465-3

Cited by 32 publications

(26 citation statements)

References 56 publications

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“…In this study, the 8 P. acidilactici strains displayed a broad antimicrobial spectrum against fish pathogens, which could be due to different antimicrobial metabolites such as organic acids (mainly lactic acid), hydrogen peroxide, diacetyl, acetaldehyde, and/or bacteriocins, with the organic acids and bacteriocins being the key weapons in inhibiting pathogens of relevance for aquaculture (Gillor et al 2008, Desriac et al 2010. In this respect, 6 out of 8 P. acidilactici strains were found to be bacteriocin producers, based on the proteinaceous nature and heat stability of the antimicrobial agent, which is in agreement with previous studies, where a remarkably high number of LAB displaying antimicrobial activity against fish pathogens were potential bacteriocin producers (Muñoz-Atienza et al 2013, Gómez-Sala et al 2015. However, the results indicated that the antimicrobial activity against L. garvieae exerted by the pediococci was not due to the production of bacteriocins, as the antimicrobial effect was only observed in the presence of pediococcal cells.…”

Section: Discussionsupporting

confidence: 89%

Characterization of Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss) feed and larvae: safety, DNA fingerprinting, and bacteriocinogenicity

Araújo¹,

Muñoz-Atienza²,

Poeta³

et al. 2016

Dis. Aquat. Org.

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The use of lactic acid bacteria (LAB) as probiotics constitutes an alternative or complementary strategy to chemotherapy and vaccination for disease control in aquaculture. The objectives of this work were (1) the in vitro safety assessment of 8 Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss, Walbaum) feed and larvae; (2) the evaluation of their genetic relatedness; (3) the study of their antimicrobial/bacteriocin activity against fish pathogens; and (4) the biochemical and genetic characterization of the bacteriocin produced by the strain displaying the greatest antimicrobial activity. Concerning the safety assessment, none of the pediococci showed antibiotic resistance nor produced hemolysin or gelatinase, degraded gastric mucin, or deconjugated bile salts. Four strains (50%) produced tyramine or putrescine, but the corresponding genes were not amplified by PCR. Enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) fingerprinting allowed clustering of the pediococci into 2 well-defined groups (68% similarity). From the 8 pediococci displaying direct antimicrobial activity against at least 3 out of 9 fish pathogens, 6 strains (75%) were identified as bacteriocin producers. The bacteriocin produced by P. acidilactici L-14 was purified, and mass spectrometry and DNA sequencing revealed its identity to pediocin PA-1 (PedPA-1). Altogether, our results allowed the identification of 4 (50%) putatively safe pediococci, including 2 bacteriocinogenic strains. ERIC-PCR fingerprinting was a valuable tool for genetic profiling of P. acidilactici strains. This work reports for the first time the characterization of a PedPA-1-producing P. acidilactici strain isolated from an aquatic environment (rainbow trout larvae), which shows interesting properties related to its potential use as a probiotic in aquaculture.

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Section: Discussionsupporting

confidence: 89%

Characterization of Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss) feed and larvae: safety, DNA fingerprinting, and bacteriocinogenicity

Araújo¹,

Muñoz-Atienza²,

Poeta³

et al. 2016

Dis. Aquat. Org.

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“…Our results are in agreement with the findings of previous studies. Gómez-Sala et al (2015) demonstrated that analysis of 1245 LAB isolates obtained from fish, seafood, and fish products showed that 197 exerted direct antimicrobial activity against 20 spoilage and food-borne pathogenic microorganisms. Furthermore, LAB isolates selected on the basis of their direct antimicrobial activity were identified as E. faecium, E. faecalis, Pediococcus pentosaceus, Weissella cibaria, L. sakei subsp.…”

Section: Resultsmentioning

confidence: 99%

Inhibitory effect of bacteriocin-producing lactic acid bacteria against histamine-forming bacteria isolated from Myeolchi-jeot

Lim

2016

Fish Aquatic Sci

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The objectives of this study were to identify the histamine-forming bacteria and bacteriocin-producing lactic acid bacteria (LAB) isolated from Myeolchi-jeot according to sequence analysis of the 16S rRNA gene, to evaluate the inhibitory effects of the bacteriocin on the growth and histamine accumulation of histamine-forming bacteria, and to assess the physico-chemical properties of the bacteriocin. Based on 16S rRNA gene sequences, histamine-forming bacteria were identified as Bacillus licheniformis MCH01, Serratia marcescens MCH02, Staphylococcus xylosus MCH03, Aeromonas hydrophila MCH04, and Morganella morganii MCH05. The five LAB strains identified as Pediococcus acidilactici MCL11, Leuconostoc mesenteroides MCL12, Enterococcus faecium MCL13, Lactobacillus sakei MCL14, and Lactobacillus acidophilus MCL15 were found to produce an antibacterial compound with inhibitory activity against the tested histamine-producing bacteria. The inhibitory activity of these bacteriocins obtained from the five LAB remained stable after incubation at pH 4.0-8.0 and heating for 10 min at 80°C; however, the bacteriocin activity was destroyed after treatment with papain, pepsin, proteinase K, α-chymotrypsin, or trypsin. Meanwhile, these bacteriocins produced by the tested LAB strains also exhibited histamine-degradation ability. Therefore, these antimicrobial substances may play a role in inhibiting histamine formation in the fermented fish products and preventing seafood-related food-borne disease caused by bacterially generated histamine.

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“…The enterococci comprised the largest LAB group with antimicrobial activity (91 %) with E. faecium (40 %) as the most identified antagonistic species. Enterococci with antimicrobial activity have been previously identified from LAB isolated from mammals, birds and fish [ 10 – 12 ], but not with this elevated percentage of isolation. Enterococci are also the most common species in cloacal and pharyngeal samples of Euroasian griffon vultures [ 26 ] and in Turkey and Black vultures whereas enterococcal symbionts living in the hoopoe ( Upua epops ) uropygial gland produce bacteriocins active against Gram-positive pathogens [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

“…Environmental sources as well as wild and game animals are a powerful source of bacteriocin-producing LAB [ 10 – 12 ]. Griffon vultures ( Gyps fulvus subspecies fulvus ) belong to the Old World vultures group, a diverse mix of colonial cliff-nesting scavenger birds that play an essential ecological role as garbage collectors and recyclers.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of bacteriocinogenic activity, safety traits and biotechnological potential of fecal lactic acid bacteria (LAB), isolated from Griffon Vultures (Gyps fulvus subsp. fulvus)

et al. 2016

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BackgroundLactic acid bacteria (LAB) are part of the gut microbiota and produce ribosomally synthesized antimicrobial peptides or bacteriocins with interest as natural food preservatives and therapeutic agents. Bacteriocin-producing LAB are also attractive as probiotics. Griffon vultures (Gyps fulvus subspecies fulvus) are scavenger birds that feed almost exclusively on carrion without suffering apparent ill effects. Therefore, griffon vultures might be considered a reservoir of bacteriocin-producing lactic acid bacteria (LAB) with potential biotechnological applications.ResultsGriffon vulture feces were screened for LAB with antimicrobial activity, genes encoding bacteriocins, potential virulence determinants, susceptibility to antibiotics, genotyping and characterization of bacteriocins. In this study, from 924 LAB evaluated 332 isolates (36 %) showed direct antimicrobial activity against Gram-positive bacteria only. The molecular identification of the most antagonistic 95 isolates showed that enterococci was the largest LAB group with antimicrobial activity (91 %) and E. faecium (40 %) the most identified antagonistic species. The evaluation of the presence of bacteriocin structural genes in 28 LAB isolates with the highest bacteriocinogenic activity in their supernatants determined that most enterococcal isolates (75 %) encoded multiple bacteriocins, being enterocin A (EntA) the largest identified (46 %) bacteriocin. Most enterococci (88 %) were resistant to multiple antibiotics. ERIC-PCR and MLST techniques permitted genotyping and recognition of the potential safety of the bacteriocinogenic enterococci. A multiple-step chromatographic procedure, determination of the N-terminal amino acid sequence of purified bacteriocins by Edman degradation and a MALDI TOF/TOF tandem MS procedure permitted characterization of bacteriocins present in supernatants of producer cells.ConclusionsEnterococci was the largest LAB group with bacteriocinogenic activity isolated from griffon vulture feces. Among the isolates, E. faecium M3K31 has been identified as producer of enterocin HF (EntHF), a bacteriocin with remarkable antimicrobial activity against most evaluated Listeria spp. and of elevated interest as a natural food preservative. E. faecium M3K31 would be also considered a safe probiotic strain for use in animal nutrition.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-016-0840-2) contains supplementary material, which is available to authorized users.

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Bacteriocin production by lactic acid bacteria isolated from fish, seafood and fish products

Abstract: suitability of fish, seafood and fish products for the isolation of LAB (mainly enterococci), including (multi)bacteriocinogenic strains, encoding bacteriocins active against Listeria monocytogenes and other food-borne pathogens of interest for the food industry.

Cited by 32 publications

References 56 publications

Characterization of Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss) feed and larvae: safety, DNA fingerprinting, and bacteriocinogenicity

Characterization of Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss) feed and larvae: safety, DNA fingerprinting, and bacteriocinogenicity

Inhibitory effect of bacteriocin-producing lactic acid bacteria against histamine-forming bacteria isolated from Myeolchi-jeot

Evaluation of bacteriocinogenic activity, safety traits and biotechnological potential of fecal lactic acid bacteria (LAB), isolated from Griffon Vultures (Gyps fulvus subsp. fulvus)

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