Bacteriocin Production by &lt;i&gt;Lactobacilli&lt;/i&gt; and Their Role as Antibacterial Tool against Common Pathogens

Younas, Samina; Mazhar, Bushra; Liaqat, Iram; Ali, Shaukat; Tahir, Hafız Muhammad; Ali, Nazish Mazhar

doi:10.5650/jos.ess21424

Cited by 10 publications

(6 citation statements)

References 18 publications

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“…The results agreed with [28] who reported the high ability of L. crispatus and L. parabuchneri to adhere with S. mutans which reached 71.66% after 5 hrs. The obtained result was also in agreement with study that reported the ability of Lactobacillus to co-aggregate with pathogens [33]. According to the definition by the World Health Organization (WHO) and the American Food and Agricultural Organization (FAO), a probiotic is "a live organism which has a beneficial effect on human health when they take in enough amounts" [34].…”

Section: Resultssupporting

confidence: 89%

Antibacterial Activity of Live Cells of Lactobacillus Plantarum L40 as A Probiotic Against Pathogens Associated with Diabetic Foot Infections

AlAsadi,

Luti

2023

Iraqi Journal of Science

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The present study was designed to select a suitable isolate of Lactobacillus sp. in order to use it as a probiotic formula for treating diabetic foot ulcer disease in diabetic mellitus patients. In order to determine the inhibitory action, 240 isolates of Lactobacillus from different sources, were taken and exposed for screening tests to evaluate the (antagonism-activities) toward 4 multi-drug resistant foot ulcer pathogens which were chosen from 120 pathogenic isolates: Staphylococcus aureus, Klebsiella pneumonia, Proteus mirabilis, and P. aeruginosa. Twenty Lactobacillus isolates were selected from primary screening according to the capacity to restrain its expansion of four markers, that were exposed toward second screenings to detect their ability to produce a high amount of bacteriocin. Based on the results, the isolate Lactobacillus L40 was selected as the highest bacteriocin producing isolate which was further characterized as Lactobacillus plantarum Next, several tests were performed to study the properties of L. plantarum L40, in particular its biosafety and its suitability to be a successful probiotic. Results showed that L. plantarum was resistant to cephalosporin groups such as ceftriaxone and cefepime whereas, it was sensitive to amikacin and chloramphenicol. In addition, results revealed that this bacterium was strongly adherent and a good biofilm producer; had high auto-aggregation capability and a high ability to adhere with S. Staphylococcus aureus, Klebsiella pneumonia, Proteus mirabilis and P. aeruginosa. A gel formula containing live cells of L. plantarum L40 was prepared and based on the results, cells were successfully survived in this formula over 18 days. Therefore, it was selected to be utilized as a vector to store and transport viable cells of L. plantarum L40. This gel formula showed antibacterial activity by inhibiting Staphylococcus aureus, Klebsiella pneumonia, Proteus mirabilis, and P. aeruginosa growth. The results showed the possibility of using viable cells of bacteriocin-producing Lactobacillus plantarum as an effective probiotic to deal with some skin pathogens, and hence treat skin diseases such as diabetic foot ulcer disease.

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Section: Resultssupporting

confidence: 89%

Antibacterial Activity of Live Cells of Lactobacillus Plantarum L40 as A Probiotic Against Pathogens Associated with Diabetic Foot Infections

AlAsadi,

Luti

2023

Iraqi Journal of Science

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“…P. acidilactici R9 and P. pentosaceus R10 also exhibited strong inhibitory abilities, with their inhibitory effects being more prominent against Gram-negative bacteria compared to Gram-positive bacteria. However, L. fermentum and L. paracasei displayed the weakest inhibitory abilities, particularly against Gram-positive bacteria, which is similar to the findings of Trindade et al (2022) and Younas et al (2022) . This is seemingly attributed to the ability of LAB in inhibiting or eradicating biofilms ( Shokri et al, 2018 ; Ibrahim et al, 2021 ), a mechanism that proves more challenging when confronted with the thicker peptidoglycan cell wall structure characteristic of Gram-positive bacteria.…”

Section: Resultssupporting

confidence: 80%

Screening and characterization of lactic acid bacteria and fermentation of gamma-aminobutyric acid-enriched bamboo shoots

Chen,

Xia,

Zuo

et al. 2024

Front. Microbiol.

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In order to produce fermented bamboo shoots with functional properties, two strains of lactic acid bacteria were selected for inoculation and fermentation. One strain, Lactiplantibacillus plantarum R1, exhibited prominent potential probiotic properties (including gastrointestinal condition tolerance, adhesion ability, antimicrobial ability, and antibiotic resistance), while the other, Levilactobacillus brevis R2, demonstrated the capability of high γ-aminobutyric acid (GABA) production (913.99 ± 14.2 mg/L). The synergistic inoculation of both strains during bamboo shoot fermentation led to a remarkable increase in GABA content (382.31 ± 12.17 mg/kg), surpassing that of naturally fermented bamboo shoots by more than 4.5 times and outperforming mono-inoculated fermentation. Simultaneously, the nitrite content was maintained at a safe level (5.96 ± 1.81 mg/kg). Besides, inoculated fermented bamboo shoots exhibited an increased crude fiber content (16.58 ± 0.04 g/100 g) and reduced fat content (0.39 ± 0.02 g/100 g). Sensory evaluation results indicated a high overall acceptability for the synergistically inoculated fermented bamboo shoots. This study may provide a strategy for the safe and rapid fermentation of bamboo shoots and lay the groundwork for the development of functional vegetable products enriched with GABA.

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“…L. lactis subp. lactis and L. plantarum mainly harbor non-enterocin genes, such as curvacin A, plantaricin A, sakacin P, and nisin [ 79 ] which might explain the absence of investigated enterocin genes in the two out of the three L. lactis subp. Lactis and the L. plantarum isolate.…”

Section: Resultsmentioning

confidence: 99%

Selection and Characterization of Bacteriocinogenic Lactic Acid Bacteria from the Intestine of Gilthead Seabream (Sparus aurata) and Whiting Fish (Merlangius merlangus): Promising Strains for Aquaculture Probiotic and Food Bio-Preservation

Cheriet,

Lengliz,

Romdhani

et al. 2023

Life

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This study sought to evaluate the probiotic properties and the food preservation ability of lactic acid bacteria isolates collected from the intestines of wild marine fishes (gilthead seabream (Sparus aurata) (n = 60) and whiting fish (Merlangius merlangus) (n = 40)) from the Mediterranean sea in the area of Mostaganem city, Algeria. Forty-two isolates were identified as: Enterococcus durans (n = 19), Enterococcus faecium (n = 15), Enterococcus faecalis (n = 4), Lactococcus lactis subp. lactis (n = 3), and Lactobacillus plantarum (n = 1). All isolates showed inhibition to at least one indicator strain, especially against Listeria monocytogenes, Staphylococcus aureus, Paenibacillus larvae, Vibrio alginolyticus, Enterococcus faecalis, Bacillus cereus, and Bacillus subtilis. In all collected isolates, PCR analysis of enterocin-encoding genes showed the following genes: entP (n = 21), ent1071A/B (n = 11), entB (n = 8), entL50A/B (n = 7), entAS48 (n = 5), and entX (n = 1). Interestingly, 15 isolates harbored more than one ent gene. Antimicrobial susceptibility, phenotypic virulence, and genes encoding virulence factors were investigated by PCR. Resistance to tetracycline (n = 8: tetL + tetK), erythromycin (n = 7: 5 ermA, 2 msrA, and 1 mef(A/E)), ciprofloxacin (n = 1), gentamicin (n = 1: aac(6′)-aph(2″)), and linezolid (n = 1) were observed. Three isolates were gelatinase producers and eight were α-hemolytic. Three E. durans and one E. faecium harbored the hyl gene. Eight isolates showing safety properties (susceptible to clinically relevant antibiotics, free of genes encoding virulence factors) were tested to select probiotic candidates. They showed high tolerance to low pH and bile salt, hydrophobicity power, and co-culture ability. The eight isolates showed important phenotypic and genotypic traits enabling them to be promising probiotic candidates or food bio-conservers and starter cultures.

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Bacteriocin Production by <i>Lactobacilli</i> and Their Role as Antibacterial Tool against Common Pathogens

Cited by 10 publications

References 18 publications

Antibacterial Activity of Live Cells of Lactobacillus Plantarum L40 as A Probiotic Against Pathogens Associated with Diabetic Foot Infections

Antibacterial Activity of Live Cells of Lactobacillus Plantarum L40 as A Probiotic Against Pathogens Associated with Diabetic Foot Infections

Screening and characterization of lactic acid bacteria and fermentation of gamma-aminobutyric acid-enriched bamboo shoots

Selection and Characterization of Bacteriocinogenic Lactic Acid Bacteria from the Intestine of Gilthead Seabream (Sparus aurata) and Whiting Fish (Merlangius merlangus): Promising Strains for Aquaculture Probiotic and Food Bio-Preservation

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