Bacteriocin release proteins: mode of action, structure, and biotechnological application

Wal, F.J. van der; Luirink, Joen; Oudega, Bauke

doi:10.1111/j.1574-6976.1995.tb00221.x

Cited by 31 publications

(4 citation statements)

References 165 publications

(216 reference statements)

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“…Group A colicins require the activity of CRPs to reach the surrounding environment. Colicin release proteins of group A colicins are small, highly expressed lipoproteins (van der Wal et al ., ). They contain a signal peptide which accumulates in the bacterial inner membrane after cleavage from the mature, lipid‐modified CRP.…”

Section: Discussionmentioning

confidence: 97%

Temperate phages promote colicin‐dependent fitness of Salmonella enterica serovar Typhimurium

Nedialkova¹,

Sidstedt²,

Koeppel

et al. 2015

Environmental Microbiology

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Bacteria employ bacteriocins for interference competition in microbial ecosystems. Colicin Ib (ColIb), a pore-forming bacteriocin, confers a significant fitness benefit to Salmonella enterica serovar Typhimurium (S. Tm) in competition against commensal Escherichia coli in the gut. ColIb is released from S. Tm into the environment, where it kills susceptible competitors. However, colicin-specific release proteins, as they are known for other colicins, have not been identified in case of ColIb. Thus, its release mechanism has remained unclear. In the current study, we have established a new link between ColIb release and lysis activity of temperate, lambdoid phages. By the use of phage-cured S. Tm mutant strains, we show that the presence of temperate phages and their lysis genes is necessary and sufficient for release of active ColIb into the culture supernatant. Furthermore, phage-mediated lysis significantly enhanced S. Tm fitness in competition against a ColIb-susceptible competitor. Finally, transduction with the lambdoid phage 933W rescued the defect of E. coli strain MG1655 with respect to ColIb release. In conclusion, ColIb is released from bacteria in the course of phage lysis. Our data reveal a new mechanism for colicin release and point out a novel function of temperate phages in enhancing colicin-dependent bacterial fitness.

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Section: Discussionmentioning

confidence: 97%

Temperate phages promote colicin‐dependent fitness of Salmonella enterica serovar Typhimurium

Nedialkova¹,

Sidstedt²,

Koeppel

et al. 2015

Environmental Microbiology

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“…In addition, our data do not rule out the possibility that the cells may not actively secrete the toxin across the outer membrane and instead, the bacteria may need to lyse to release the toxin. Cell lysis is required for the release of many colicins 38–41 .…”

Section: Discussionmentioning

confidence: 99%

A family of anti-Bacteroidales peptide toxins wide-spread in the human gut microbiota

et al. 2019

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Bacteria often produce antimicrobial toxins to compete in microbial communities. Here we identify a family of broad-spectrum peptide toxins, named bacteroidetocins, produced by Bacteroidetes species. We study this toxin family using phenotypic, mutational, bioinformatic, and human metagenomic analyses. Bacteroidetocins are related to class IIa bacteriocins of Gram-positive bacteria and kill members of the Bacteroidetes phylum, including Bacteroides , Parabacteroides , and Prevotella gut species, as well as pathogenic Prevotella species. The bacteroidetocin biosynthesis genes are found in horizontally acquired mobile elements, which likely allow dissemination within the gut microbiota and may explain their wide distribution in human populations. Bacteroidetocins may have potential applications in microbiome engineering and as therapeutics for polymicrobial diseases such as bacterial vaginosis and periodontal disease.

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“…This lack of suppression could be due to the failure of the release of SyrM protein. It has been shown that the release of some colicins is regulated by colicin release protein (CRP)‐mediated cell lysis (Wal et al ., ; Cascales et al ., ) or during the course of phage lysis (Nedialkova et al ., ). As no SyrM‐associated lysis gene was identified in Pst , we thus speculate that this bacteriocin is released through an alternative mechanism which is still obscure at present.…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas bacteriocin syringacin M released upon desiccation suppresses the growth of sensitive bacteria in plant necrotic lesions

Zhou

Peng

et al. 2019

Microbial Biotechnology

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SummaryBacteriocins are regarded as important factors mediating microbial interactions, but their exact role in community ecology largely remains to be elucidated. Here, we report the characterization of a mutant strain, derived from Pseudomonas syringae pv. tomato DC3000 (Pst), that was incapable of growing in plant extracts and causing disease. Results showed that deficiency in a previously unannotated gene saxE led to the sensitivity of the mutant to Ca2+ in leaf extracts. Transposon insertions in the bacteriocin gene syrM, adjacent to saxE, fully rescued the bacterial virulence and growth of the ΔsaxE mutant in plant extracts, indicating that syrM‐saxE encode a pair of bacteriocin immunity proteins in Pst. To investigate whether the syrM‐saxE system conferred any advantage to Pst in competition with other SyrM‐sensitive pathovars, we compared the growth of a SyrM‐sensitive strain co‐inoculated with Pst strains with or without the syrM gene and observed a significant syrM‐dependent growth reduction of the sensitive bacteria on plate and in lesion tissues upon desiccation–rehydration treatment. These findings reveal an important biological role of SyrM‐like bacteriocins and help to understand the complex strategies used by P. syringae in adaptation to the phyllosphere niche in the context of plant disease.

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Bacteriocin release proteins: mode of action, structure, and biotechnological application

Cited by 31 publications

References 165 publications

Temperate phages promote colicin‐dependent fitness of Salmonella enterica serovar Typhimurium

Temperate phages promote colicin‐dependent fitness of Salmonella enterica serovar Typhimurium

A family of anti-Bacteroidales peptide toxins wide-spread in the human gut microbiota

Pseudomonas bacteriocin syringacin M released upon desiccation suppresses the growth of sensitive bacteria in plant necrotic lesions

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