Bacteriocins: Biological tools for bio-preservation and shelf-life extension

Deegan, Lucy H.; Cotter, Paul D.; Hill, Colin; Ross, R. Paul

doi:10.1016/j.idairyj.2005.10.026

Cited by 582 publications

(478 citation statements)

References 112 publications

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“…The similar results were reported in previous studies that most enterocin producing enterococci displayed antilisterial activity, while a fewer portion of them also showed antibacterial spectrum activity against Staphylococcus spp., Bacillus spp. and Clostridium spp [3,10,[16][17][18]. No correlation was found between the origin of the strains and the inhibitory spectrum.…”

Section: Resultsmentioning

confidence: 92%

Phenotypic and Genotypic Characterization of Bacteriocins in Enterococcal Isolates of Different Sources

et al. 2011

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A collection of 57 enterococcal isolates from different origin (including river, treatment plant, spring and garbage water, soil, animal, and vegetables from Aydın) was screened for the production of bacteriocins. Enterococci were identified at species levels as Enterococcus faecium (34), E. hirae (6), E. casseliflavus (4), E. durans (4), E. faecalis (4), E. mundtii (3) and E. avium (2). Of the 57 isolates 40 of them inhibited the growth of at least one indicator bacterium. Based on our PCR results 54 strains possesed enterocin genes. The genes of entA and entB were the most frequently detected structural genes among the PCR positive strains (54 and 53 strains, respectively) and the entB gene was always associated with entA gene. The highest combination of enterocin genes (24 of 54 strains) detected was entA, entB, entP and entL50A/B. The enterocins AS-48 and CylL LS genes were not found. Three enterococcal isolates, 2 E. faecium and 1 E. hirae were not harbour any of tested enterocin genes. No correlation between the presence of enterocin structural genes and the origin of the strain was detected, also no relationship seemed to exist between the tested enterocin genes and the activity spectra of isolates. Genes encoding bacteriocins are widely disseminated among enterocci from different origin and more studies should be done for evaluate industrial potential of bacteriocins.

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Section: Resultsmentioning

confidence: 92%

Phenotypic and Genotypic Characterization of Bacteriocins in Enterococcal Isolates of Different Sources

et al. 2011

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“…Within this group, L. monocytogenes is a difficult pathogen to control because of its ubiquitous distribution, tolerance to high levels of salt and its stability to grow at a relatively low pH and at refrigeration temperatures (Guinane, Cotter, Hill, & Ross, 2005). The anti-listerial effects of LAB are a wanted characteristic, since it strongly contributes to the safety of the final foods (Deegan, Cotter, Hill, & Ross, 2006). The three strains behaved similarly and they inhibited only a few species within the pro-technological bacterial group, but they were ineffective against Gram-negative bacteria, at least in the non concentrated form.…”

Section: Discussionmentioning

confidence: 99%

Production, stability, gene sequencing and in situ anti-Listeria activity of mundticin KS expressed by three Enterococcus mundtii strains

Settanni¹,

Guarcello²,

Gaglio³

et al. 2014

Food Control

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a b s t r a c tThree enterococci (WFE3, WFE20 and WFE31) selected as presumptive bacteriocin producers were found to be active against Listeria monocytogenes. In this study, due to their potential industrial/food applications, the three bacterial isolates were extensively characterized. Identification was performed by means of a combined 16S rRNA gene sequencing and multiplex PCR approach, and was confirmed with the sequencing of a partial region of a protein-encoding gene, namely pheS. The three isolates belonged unequivocally to the species Enterococcus mundtii. The randomly amplified polymorphic DNA (RAPD) analysis recognized three distinct strains. The supernatants were mainly active against Listeria spp., but some lactic acid bacteria were also inhibited. The proteinaceous nature of the three supernatants was detected after treatment with proteinase K, protease B and trypsin. The bacteriocins were found to be heat resistant, stable in a large pH range and in presence of ethanol. The bacteriocins were not adsorbed onto the surface of the producer cells and their effect was bactericidal. The production of bacteriocins was higher at neutral pHs and temperatures in the range 30e37 C. The active supernatants did not show cytotoxicity against human erythrocytes and the three strains were susceptible to the action of common antibiotics. The genetic characterization of the bacteriocin genes showed that all three strains produced mundticin KS. They produced it in five food model systems, sterilized by thermal treatment or filtration, prepared from fresh vegetables, cereals, cheeses, meats and fishes. The in situ anti-listerial activities of the strains WFE3, WFE20 and WFE31 were quantitatively different.

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“…Currently, BLS have gaining increased attention due to their potential use as natural preservatives in the food industry, due to activity against many pathogenic bacteria and the highest activity against Listeria species [14,15]. An example is a nisin, a non-toxic bacteriocin hydrolyzed by digestive enzymes that have been used for over 50 y as a food preservative in different countries without the substantial development of bacterial resistance [16,17]. Until recently, it was believed that bacteria could not acquire resistance to antimicrobial peptides, so these substances would be strong candidates for new preservative in foods [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Influence of Peptide P34 on Gene Expression of Listeria Monocytogenes and Listeria Seelegeri

Vaucher

Giongo²,

Estivales

et al. 2016

Int J Pharm Pharm Sci

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Objective: Investigate the influence of the antimicrobial peptides P34 and nisin on the expression of genes associated with components of the cell surface of Listeria monocytogenes and Listeria seeligeri.Methods: Antimicrobial activity was determined by addition of peptide P34 and nisin (12.5 µg/ml) onto Brain Heart Infusion agar (BHI) plates previously inoculated with indicator strains (L. monocytogenes ATCC 7644 or L. seeligeri AC 82/4) after incubation for 24 h at 37 °C or 240 h at 4 °C. Ribonucleic acid (RNA) was directly extracted from bacterial colonies at the border of the inhibition zones, and the expression levels of genes Dalanine-D-alanyl carrier protein ligase (dltA), putative phospholipid lysinylation (Imo 1695) and EIIAB Man Results: A non-significant increase in the levels of transcription of genes dltA, Imo1695 and mptA was observed for L. monocytogenes treated with peptide P34 or nisin. Both peptides caused a similar decrease in dltA gene expression in L. seeligeri. The expression of gene Imo1695 significantly decreased (about 2000-fold) after treatment with the peptide P34 at 37 °C, while at 4 °C a reduction of 12-fold and 5-fold was detected for P34 and nisin, respectively. A significant decrease in mptA gene expression was observed by exposition to peptide P34 (31.872-fold) and nisin (16.047-fold) for 24 h at 37 °C.of mannose-specific PTS (mptA) were determined using real-time PCR. Conclusion:The results suggest that both peptide P34 and nisin influence the expression of genes related with the cell-surface/cell-membrane structure of L. seeligeri and in lesser extent L. monocytogenes.

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Bacteriocins: Biological tools for bio-preservation and shelf-life extension

Cited by 582 publications

References 112 publications

Phenotypic and Genotypic Characterization of Bacteriocins in Enterococcal Isolates of Different Sources

Phenotypic and Genotypic Characterization of Bacteriocins in Enterococcal Isolates of Different Sources

Production, stability, gene sequencing and in situ anti-Listeria activity of mundticin KS expressed by three Enterococcus mundtii strains

Influence of Peptide P34 on Gene Expression of Listeria Monocytogenes and Listeria Seelegeri

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