Bacteriocins of Enterococcus

Vuyst, Luc De

doi:10.1007/978-1-4615-2668-1_23

Cited by 11 publications

(5 citation statements)

References 21 publications

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“…Enterococci are known to produce various bacteriocins (De Vuyst 1994), proteinaceous compounds with bactericidal activity usually directed against species closely related to the producing bacterium (Tagg et al . 1976).…”

Section: Introductionmentioning

confidence: 99%

Population dynamics and antagonistic potential of enterococci colonizing the phyllosphere of grasses

Ott

Müller

et al. 2001

J Appl Microbiol

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Aims: To investigate the spatial and temporal dynamics of enterococci colonizing forage grass and their ability to produce bacteriocins. Methods and Results: Enterococci could be detected on above-ground plant parts throughout the growing season, with high continuity but low cell numbers (2á60´10 1 ± 6á16´10 4 cfu g ±1 fresh matter). A total of 750 strains were isolated and identi®ed by their whole-cell protein patterns as Enterococcus faecalis (7á9%), Ent. mundtii (7á9%), Ent. casseli¯avus (5á5%), Ent. faecium (5á2%) and Ent. sulfureus (0á1%). The vast majority of the strains (69á7%) formed a homogeneous 16S rDNA genotype that differed from those of known enterococci. A screening for antagonistic activity using an agar spot test revealed that 18á4% of all isolates were potential antagonists. Partially-puri®ed proteins extracted from cell-free culture supernatant uids of various species were characterized as pH-and heat-stable bacteriocins active against a wide range of lactic acid bacteria, clostridia and Listeria. The producing strains were antagonistically active even on`phylloplane agar' at temperatures between 4 and 37°C. Conclusions: Enterococci are a common part of the epiphytic micro¯ora of grasses, displaying probably some antagonistic activity. Signi®cance and Impact of the Study: The results provide new information on the distribution, species diversity and antagonistic potential of enterococci in the phyllosphere.

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Section: Introductionmentioning

confidence: 99%

Population dynamics and antagonistic potential of enterococci colonizing the phyllosphere of grasses

Ott

Müller

et al. 2001

J Appl Microbiol

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“…faecium and Ent. faecalis (reviewed by De Vuyst 1994 and Hill 1994), many of which have not been fully characterized. The best‐studied enterococcal bacteriocins include enterocins E1A and E1B, DPC 1146 and Sf3, produced by Ent.…”

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confidence: 99%

Enterocin 012, a bacteriocin produced by Enterococcus gallinarum isolated from the intestinal tract of ostrich

2000

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Enterococcus gallinarum strain 012, isolated from the duodenum of ostrich, produced enterocin 012 which is active against Ent. faecalis, Lactobacillus acidophilus, Lact. sake, Listeria innocua, Propionibacterium acidipropionici, Propionibacterium sp., Clostridium perfringens, Pseudomonas aeruginosa and Salmonella typhimurium. One of the four pathogenic strains of Escherichia coli isolated from the intestinal tract of ostrich was inhibited by enterocin 012. No antimicrobial activity was recorded against Bacillus cereus, Cl. sporogenes, Cl. tyrobutyricum, Leuconostoc cremoris, Pediococcus pentosaceus, Staphylococcus carnosus and Streptococcus thermophilus. Enterocin 012 was resistant to treatment with lysozyme, catalase, lipase and papain, but sensitive to Proteinase K, α‐chymotrypsin, trypsin and pepsin. Treatment of enterocin 012 with gastric juice from the duodenum resulted in a 50% loss of antibacterial activity. Half of the activity was lost when incubated at 80 °C for 30 min, or when kept overnight at a pH of 1·0–5·0 and pH 11·0 and 12·0, respectively. Enterocin 012 production started in mid‐logarithmic growth and reached a maximum of 800 AU ml−1, but increased further to 1600 AU ml−1 in the stationary growth phase. The peptide is approximately 3·4 kDa in size, as determined after partial purification with Amberlite XAD‐1180 and ammonium sulphate precipitation, followed by tricine‐sodium dodecyl sulphate‐polyacrylamide gel electrophoresis. The mechanism of antimicrobial activity against Lact. sake LMG 13558 is bactericidal and caused cell lysis of active growing cells.

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“…Enterococcus has been the subject of much research given its members' potential as biopreservatives. Some strains isolated from cheese produce enterocins (bacteriocins); their bactericidal activity may be effective against foodborne pathogens such as Listeria monocytogenes, Vibrio cholerae, Staphylococcus aureus, Clostridium botulinum, Salmonella enterica and Bacillus cereus, as well as against spoilage microorganisms [4][5][6][7][8]. Indeed, non-pathogenic enterococci have promising commercial potential [9].…”

Section: Introductionmentioning

confidence: 99%

An agmatine-inducible system for the expression of recombinant proteins in Enterococcus faecalis

et al. 2014

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Background: Scientific interest in Enterococcus faecalis has increased greatly over recent decades. Some strains are involved in food fermentation and offer health benefits, whereas others are vancomycin-resistant and cause infections that are difficult to treat. The limited availability of vectors able to express cloned genes efficiently in E. faecalis has hindered biotechnological studies on the bacterium's regulatory and pathogenicity-related genes. The agmatine deiminase (AGDI) pathway of E. faecalis, involved in the conversion of agmatine into putrescine, is driven by a response inducer gene aguR.Results: This study describes that the exposure to the induction factor (agmatine) results in the transcription of genes under the control of the aguB promoter, including the aguBDAC operon. A novel E. faecalis expression vector, named pAGEnt, combining the aguR inducer gene and the aguB promoter followed by a cloning site and a stop codon was constructed. pAGEnt was designed for the overexpression and purification of a protein fused to a 10-amino-acid His-tag at the C-terminus. The use of GFP as a reporter of gene expression in E. faecalis revealed that under induction with 60 mM agmatine, fluorescence reached 40 arbitrary units compared to 0 in uninduced cells. Conclusion: pAGEnt vector can be used for the overexpression of recombinant proteins under the induction of agmatine in E. faecalis, with a close correlation between agmatine concentration and fluorescence when GFP was used as reporter.

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Bacteriocins of Enterococcus

Cited by 11 publications

References 21 publications

Population dynamics and antagonistic potential of enterococci colonizing the phyllosphere of grasses

Population dynamics and antagonistic potential of enterococci colonizing the phyllosphere of grasses

Enterocin 012, a bacteriocin produced by Enterococcus gallinarum isolated from the intestinal tract of ostrich

An agmatine-inducible system for the expression of recombinant proteins in Enterococcus faecalis

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