Bacteriocins Reduce Campylobacter Colonization and Alter Gut Morphology in Turkey Poults ,

Abstract

Campylobacter is a leading cause of food-borne illness in the United States. Recent evidence has demonstrated that bacteriocins produced by Bacillus circulans and Paenibacillus polymyxa reduce cecal Campylobacter colonization in broiler chickens infected with Campylobacter jejuni. As Campylobacter coli is the most prevalent Campylobacter isolate recovered in turkeys, the objectives of the present study were to evaluate the efficacy of these bacteriocins against C. coli colonization and their influence on the gastrointestinal architecture of young turkeys. In 3 separate trials, a total of 135 day-of-hatch poults (n = 45/trial) were orally challenged on d 3 with approximately 10(6) cfu of a mixture of 3 C. coli isolates. Immediately before bacteriocin treatment (d 10), cecal Campylobacter concentrations averaged 1.1 x 10(7) cfu/ g of cecal contents (n = 15/trial). On d 10 to 12 posthatch, 2 bacteriocin treatment groups were given free access to feed supplemented with purified, microencapsulated bacteriocins, whereas the positive control treatment group had access to untreated feed (n = 10/treatment group per trial). At the end of the 3-d dosing period, ceca and duodenal loops were collected for analysis. In each of the 3 separate trials, treatment with bacteriocin eliminated detectable ceca Campylobacter concentrations (detection limit, 1 x 10(2) cfu/g of cecal contents) vs. controls (1.0 x 106 cfu of Campylobacter/g of cecal contents). Duodenum crypt depth and goblet cell numbers were also reduced in turkeys treated with either bacteriocin vs. controls (P < 0.05). The dynamic reduction in crypt depth and goblet cell density in turkeys dosed with bacteriocin may provide clues to how bacteriocins inhibit enteric Campylobacter.

Therapeutic Supplementation of Caprylic Acid in Feed Reduces Campylobacter jejuni Colonization in Broiler Chicks

“…Based on previous morphometric analysis, we postulated that another possible mechanism of action of caprylic acid may be a physical or functional alteration of the gastrointestinal colonization site of C. jejuni in chicks. Our previous research with poults fed bacteriocins found that the reduction in Campylobacter counts was associated with an alteration in the preferential gastrointestinal colonization sites for this organism (7). In the present study, caprylic acid supplementation showed either no effects or inconsistent effects on gastrointestinal morphology (data not shown), suggesting that other mechanisms are responsible for the reduction observed for cecal C. jejuni counts.…”

Fate of the Two-Component Lantibiotic Lacticin 3147 in the Gastrointestinal Tract

“…Recently, Corr et al (6) demonstrated that the antilisterial effect of Lactobacillus salivarius UCC118 in mice was mediated by Abp118, a bacteriocin produced by the strain. Others have demonstrated that encapsulated bacteriocins are effective in reducing Campylobacter carriage in poultry and that colicinproducing Escherichia coli reduce E. coli O157 shedding in cattle (5,24,25). However, some bacteriocin-producing strains lack efficacy in vivo (2,10).…”

The dose response effects of liquid and lyophilized <i>Carnobacterium divergens</i> AS7 bacteriocin on the nutrient retention and performance of broiler chickens

“…The in vivo performance trial demonstrated positive effects for this peptide, which improved the growth and feed utilization of broiler chickens that had been challenged with Clostridium perfringens. In a study by Cole et al (2006), a significant reduction in Campylobacter jejuni in turkey poults was observed after the dietary addition of bacteriocin B602 from Paenibacillus polymyxa (NRRL B-30509) and bacteriocin OR7 from Lactobacillus salivarius .…”