Bacteriologic investigation of the effects of sodium hypochlorite and chlorhexidine during the endodontic treatment of teeth with apical periodontitis

Siqueira, Jr . José F.; Rôças, Isabela N.; Paiva, Simone S.M.; Guimarães-Pinto, Tatiana; Magalhães, Karen Machado; Lima, Kênio Costa de

doi:10.1016/j.tripleo.2007.01.027

Cited by 146 publications

(127 citation statements)

References 64 publications

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“…This finding is consistent with the results of other investigators, who showed no differences in bactericidal efficacy between NaOCl and CHX 10,[39][40][41][42] . CTR is a cationic detergent that has an antibacterial activity against many gram-positive and gram-negative bacteria 41) .…”

Section: Discussionsupporting

confidence: 93%

Antibacterial effect of chlorhexidine-cetrimide combination, <i>Salvia officinalis</i> plant extract and octenidine in comparison with conventional endodontic irrigants

Güneşer

Akbulut

Eldeniz

2016

Dental Materials Journal

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Section: Discussionsupporting

confidence: 93%

Antibacterial effect of chlorhexidine-cetrimide combination, <i>Salvia officinalis</i> plant extract and octenidine in comparison with conventional endodontic irrigants

Güneşer

Akbulut

Eldeniz

2016

Dental Materials Journal

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“…Chlorhexidine (CHX) has been recommended as an alternative to NaOCl during root canal preparation, especially in cases of open apex, root resorption, foramen enlargement and root perforation, due to its biocompatibility, or in cases of allergy related to bleaching solutions 9 . Clinical investigations have reported that CHX and NaOCl have comparable effects in eliminating bacteria [10][11] . However, only CHX has a property known as substantivity -capacity to adsorb to surfaces and maintain a prolonged antimicrobial activity 12 .…”

Section: Introductionmentioning

confidence: 99%

Resin-based sealer penetration into dentinal tubules after the use of 2% chlorhexidine gel and 17% EDTA: in vitro study

Sabadin¹,

Böttcher²,

Hoppe³

et al. 2014

Braz. J. Oral Sci.

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Aim: To evaluate, by confocal laser scanning microscopy (CLSM), the influence of 17% EDTA final irrigation on the penetration of an endodontic resin-based sealer into dentinal tubules after use of 2% chlorhexidine gel. Methods: Forty extracted bovine incisors were instrumented according to the groups: G1 -root canal preparation with 2% chlorhexidine gel (n=10); G2 -root canal preparation with 2% chlorhexidine gel and final irrigation with 17% EDTA (n=10); G3 -root canal preparation with saline and final irrigation with 17% EDTA (n=10); G4 -root canal preparation with saline (n=10). The samples were filled with gutta-percha using AH Plus sealer with rhodamine B fluorescent dye. After seven days, the teeth were sectioned at the coronal, middle, and apical thirds and viewed under confocal microscope. The most representative area of penetration depth was measured in each group. Statistical significance for the sealer penetration area was determined among groups using one-way ANOVA followed by Tuckey test. For thirds comparison, in each group, data were statistically analyzed using Friedman test (p<0.05). Results:The maximum penetration was provided by G2 -2% chlorhexidine + EDTA (p=0.000). According to this criterion, no differences were found among the other groups and among thirds within the same group. Conclusions: Based on these results, the use of 17% EDTA should be indicated after root canal preparation with 2% chlorhexidine gel for smear layer removal, enhancing the AH Plus sealer penetration.

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“…Streptococcus species have commonly been detected in samples taken immediately after endodontic treatment procedures (3,4,18,19,21,28) and in root canal-treated teeth undergoing retreatment (6,8,11,12,26,30). This suggests that these bacteria may be involved with persistent infections that result in persistent disease.…”

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confidence: 99%

Characterization of Microbiota of Root Canal-Treated Teeth with Posttreatment Disease

2012

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This study evaluated the microbiota of root canals undergoing retreatment. The most prevalent taxa detected by checkerboard included Propionibacterium species, Fusobacterium nucleatum, streptococci, and Pseudoramibacter alactolyticus. Quantitative real-time PCR detected Enterococcus faecalis and streptococci in 38% and 41% of the cases, comprising 9.76% and 65.78% of the total bacterial counts, respectively. The findings call into question the status of E. faecalis as the main pathogen and suggest that other species can be candidate pathogens associated with persistent/secondary endodontic infections. P osttreatment apical periodontitis is an inflammatory disease caused by a persistent/secondary infection of the dental root canal (8,11,13,15,26,30). Enterococcus faecalis has been the most frequently detected species in root canal-treated teeth as revealed by both culture and molecular studies (5,8,11,12,16,23,24,26,30,31), but recent studies have called into question its etiologic role in posttreatment disease (7,15,20,31). Moreover, taxa related to several other genera, including Streptococcus, Dialister, Fusobacterium, Filifactor, Parvimonas, Prevotella, Propionibacterium, and Pyramidobacter, have also been detected in treated teeth (5,8,11,12,15,20,22,26,27,29,30).The purpose of this molecular study was 2-fold. First, the samples from root canal-treated teeth with posttreatment apical periodontitis undergoing retreatment were surveyed for the presence of 28 bacterial taxa using the reverse capture-checkerboard DNA-DNA hybridization approach. Then, the total bacterial counts and the presence, levels, and proportions of E. faecalis and streptococci were determined by using a quantitative real-time PCR (qPCR) assay.Forty-two teeth were selected from patients (30 females and 12 males aged 16 to 70 years; mean age, 41 years) who had been referred for root canal retreatment to the Department of Endodontics, Estácio de Sá University. All the root canal-treated teeth were asymptomatic, showed radiographic evidence of apical periodontitis, and had had endodontic therapy completed more than 2 years earlier. All teeth were coronally restored and no direct exposure of the root canal filling material to the oral cavity was evident. The termini of the root canal fillings ranged from 0 to 4 mm short of the radiographic apex. No case was overfilled. The selected teeth showed an absence of periodontal pockets Ͼ4 mm in size. Approval for the study protocol was obtained from the Ethics Committee of the Está-cio de Sá University.The procedures for disinfecting the operative field and taking samples from treated root canals were as described previously (22, 26). Eight participants (out of 50 patients that started the experiment) were excluded because control samples from the operative field were positive for bacterial DNA as determined by broad-range PCR. DNA was extracted from clinical samples using the QIAamp DNA minikit (Qiagen, Valencia, CA), following the protocol recommended by the manufacturer. To maximize DNA extraction, ...

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Bacteriologic investigation of the effects of sodium hypochlorite and chlorhexidine during the endodontic treatment of teeth with apical periodontitis

Cited by 146 publications

References 64 publications

Antibacterial effect of chlorhexidine-cetrimide combination, <i>Salvia officinalis</i> plant extract and octenidine in comparison with conventional endodontic irrigants

Antibacterial effect of chlorhexidine-cetrimide combination, <i>Salvia officinalis</i> plant extract and octenidine in comparison with conventional endodontic irrigants

Resin-based sealer penetration into dentinal tubules after the use of 2% chlorhexidine gel and 17% EDTA: in vitro study

Characterization of Microbiota of Root Canal-Treated Teeth with Posttreatment Disease

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