Bacteriological analysis of Neisseria lactamica isolated from the respiratory tract in Japanese children

Takei, Haruka; Takeuchi, Noriko; Hoshino, Tadashi; Ohkusu, Misako; Segawa, Shunsuke; Murata, Shota; Ishiwada, Naruhiko

doi:10.1016/j.jiac.2020.08.011

Cited by 5 publications

(2 citation statements)

References 23 publications

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“…In the last decade, however, few surveys have reported data on antimicrobial susceptibility of non-pathogenic Neisseria isolates. Two studies reported high minimum inhibitory concentrations (MICs) for macrolides, cephalosporins and fluoroquinolones among N. lactamica isolates from children in Japan and China in 2015 14 , 15 . One study found 93% fluoroquinolone resistance among commensal Neisseria from asymptomatic N. meningitidis carriers in China 7 .…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial susceptibility of commensal Neisseria in a general population and men who have sex with men in Belgium

Laumen

Dijck

Abdellati

et al. 2022

Sci Rep

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Non-pathogenic Neisseria are a reservoir of antimicrobial resistance genes for pathogenic Neisseria meningitidis and Neisseria gonorrhoeae. Men who have sex with men (MSM) are at risk of co-colonization with resistant non-pathogenic and pathogenic Neisseria. We assessed if the antimicrobial susceptibility of non-pathogenic Neisseria among MSM differs from a general population and if antimicrobial exposure impacts susceptibility. We recruited 96 participants at our center in Belgium: 32 employees, 32 MSM who did not use antibiotics in the previous 6 months, and 32 MSM who did. Oropharyngeal Neisseria were cultured and identified with MALDI-TOF–MS. Minimum inhibitory concentrations for azithromycin, ceftriaxone and ciprofloxacin were determined using E-tests® and compared between groups with non-parametric tests. Non-pathogenic Neisseria from employees as well as MSM were remarkably resistant. Those from MSM were significantly less susceptible than employees to azithromycin and ciprofloxacin (p < 0.0001, p < 0.001), but not ceftriaxone (p = 0.3). Susceptibility did not differ significantly according to recent antimicrobial exposure in MSM. Surveilling antimicrobial susceptibility of non-pathogenic Neisseria may be a sensitive way to assess impact of antimicrobial exposure in a population. The high levels of antimicrobial resistance in this survey indicate that novel resistance determinants may be readily available for future transfer from non-pathogenic to pathogenic Neisseria.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial susceptibility of commensal Neisseria in a general population and men who have sex with men in Belgium

Laumen

Dijck

Abdellati

et al. 2022

Sci Rep

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“…Commensal Neisseria are an important part of the normal oropharyngeal human microbiome [ 1 ]. Various lines of evidence suggest that the prevalence of antimicrobial resistance in commensal Neisseria is increasing over time [ 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 ]. This finding is worrisome, as commensal Neisseria provide a reservoir of resistance genes that can be horizontally transferred to pathogenic bacteria, such as Neisseria gonorrhoeae and N. meningitidis, via transformation [ 9 , 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal Neisseria—A Pilot Study

et al. 2022

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Commensal Neisseria provide a reservoir of resistance genes that can be transferred to the pathogens Neisseria gonorrhoeae and N. meningitidis in the human oropharynx. Surveillance programs are thus needed to monitor resistance in oropharyngeal commensal Neisseria, but currently the isolation and antimicrobial susceptibility testing of these commensals is laborious, complex and expensive. In addition, the posterior oropharyngeal/tonsillar swab, which is commonly used to sample oropharyngeal Neisseria, is poorly tolerated by many individuals. We evaluated an alternative non-invasive method to isolate oropharyngeal commensal Neisseria and to detect decreased susceptibility to azithromycin using selective media (LBVT.SNR) with and without azithromycin (2 µg/mL). In this pilot study, we compared paired posterior oropharyngeal/tonsillar swabs and oral rinse-and-gargle samples from 10 participants and demonstrated that a similar Neisseria species diversity and number of colonies were isolated from both sample types. Moreover, the proportion of Neisseria colonies that had a decreased susceptibility to azithromycin was similar in the rinse samples compared to the swabs. This pilot study has produced encouraging data that a simple protocol of oral rinse-and-gargle and culture on plates selective for commensal Neisseria with and without a target antimicrobial can be used as a surveillance tool to monitor antimicrobial susceptibility in commensal oropharyngeal Neisseria. Larger studies are required to validate these findings.

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Neisseria

Lâm,

Vogel,

Claus

2023

ClinMicroNow

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Microaerobic growth by denitrification of nitrite via nitric oxide has also been shown for Neisseria meningitidis . The species N. gonorrhoeae and N. meningitidis are exclusively human pathogens. Identification of the serogroup of invasive N. meningitidis isolates is of interest with respect to vaccination of close contacts of the index case. A further level of differentiation to analyze local epidemiology is achieved by DNA sequence‐based typing schemes of hypervariable outer membrane proteins, which have been harmonized throughout Europe. Cultivation of N. gonorrhoeae is best performed on chocolate agar, which supports the growth of many other commensal bacteria. DNA‐based methods used for typing of N. gonorrhoeae include gel‐based methods such as opa typing and restriction fragment length polymorphism analysis by pulsed‐field gel electrophoresis. Serologic tests are used for the determination of protection against invasive meningococcal disease after vaccination or for seroepidemiological studies. First‐catch urine is recommended for the molecular detection of genitourinary N. gonorrhoeae infections in men.

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Bacteriological analysis of Neisseria lactamica isolated from the respiratory tract in Japanese children

Cited by 5 publications

References 23 publications

Antimicrobial susceptibility of commensal Neisseria in a general population and men who have sex with men in Belgium

Antimicrobial susceptibility of commensal Neisseria in a general population and men who have sex with men in Belgium

A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal Neisseria—A Pilot Study

Neisseria

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