The aim of the study was to determine the physicochemical characteristics, metal levels and anion content of water samples collected from Abakaliki, Ebonyi and Ikwo Local Government Area of Ebonyi State, Nigeria. A total of 736 water samples, comprising of 215, 111, 144, 117 and 149 from boreholes, ponds, rivers, streams and wells respectively, were collected from three local governments areas (Abakaliki, Ebonyi and ikwo) of Ebonyi State over a period of eight (8) months. Samples were analysed for physicochemical, metal, anion and microbiological characteristics using standard scientific procedures. Results revealed varying ranges in alkalinity (140.60 ± 0.20 -368.50 ± 0.20mgL -1 ), BOD (1.20 ± 0.12 -13.36 ± 0.05mgL -1 ), COD (3.14 ± 0.95 -14.20 ± 0.05mgL -1 ), conductivity (2.30 ± 0.40 -0.01 ± 0.12msL -1 ), DO (0.02 ± 1.11 -7.50 ± 0.20mgL -1 ), ph (5.80 ± 0.00 -7.83 ± 0.40), salinity (91.59 ± 0.50 -221.20 ± 0.20%), temperature (20.01 ± 1.50 -29.00 ± 1.50 0 C), TSS (89.11 ± 0.50 -750.00 ± 1.80mgL -1 ), TH (72.80 ± 0.00 -262.30 ± 0.10mgL -1 ), TOC (0.64 ± 1.50 -5.50 ± 0.60mgL -1 ), TSS (152.10 ± 1.20 -485.90 ± 0.20mgL -1 ), turbidity (3.38 ± 0.98 -11.40 ± 0.81 NTU), aluminium (0.01 ± 0.00 -0.22 ± 0.02mgL -1 ), arsenic (0.01 ± 0.01 -0.06 ± 0.01mgL -1 ), cadmium (0.02 ± 0.00 -0.40 ± 0.00mgL -1 ), calcium (20.00 ± 0.02 -68.00 ± 0.00mgL -1 ), chromium (0.01 ± 0.00 -0.09 ± 0.00mgL -1 ), copper (0.20 ± 0.01 -0.80 ± 0.00mgL -1 ), iron (0.20 ± 0.00 -0.88 ± 0.00mgL -1 ), lead (0.02 ± 0.00 -0.19 ± 0.02mgL -1 ), chlorides (40.00 ± 0.01 -265.00 ± 0.00mg/L), nitrates (11.86 ± 0.16 -65.00 ± 0.02mg/L) and phosphates (0.08 ± 0.08 -16.00 ± 0.00mg/L). In conclusion, this investigation revealed that the levels of physicochemical parameters in the water samples were beyond NSDWQ and who recommended limits signifying a potential problem for human and animal health. It is imperative to monitor the quality of water and strict quality control measures should be strengthened to ensure the effective treatment of drinking water. Educative programmes should be organized by researchers, and government agencies to enlighten the villagers on the proper use of water and the potential dangers associated with human consumption of contaminated water.