Bacteriological Investigation of Pathogenic Bacteria Causing Urinary Tract Infections: A cross-Sectional Study

A total of 153 samples (swabs) were taken from burned patients between the ages of 2 and 75, representing a variety of age groups and body regions. All samples were obtained from Baghdad’s government hospitals. Beginning in early September 2022 and ending in early January 2023, (48/153) isolates (31.37%) were identified as P. aeruginosa Testing for antibiotic susceptibility It was conducted using various antibiotic classes (8 antibiotics), which were assessed using the VITEK 2 compact system. The results revealed that P. aeruginosa isolates were resistant to ceftazidime and cefepime (100%) in the same rate, the resistance to Tobramycin and Ciprofloxacin was (77.08), and the maximum sensitivity to Colistin was )79.1) and resistance to imipenem, meropenem, and Levofloxacin was (52.08%), (79.18%), and (72.92%), respectively. In this study used zirconium nanoparticles for inhibition growth bacteria. The efficacy of the synthetic nanoparticles against P. aeruginosa was tested as five different concentrations (1,2.5,5,10 and 15) mg/ml were adopted, and concentrations of (5,10 and 15) mg/ml showed efficacy in inhibiting bacterial growth while (1 and 2.5) mg/ml was not given any effectiveness, the results of minimum inhibitory concentration was (5) mg\ml.

Effect nanoparticles zirconium on bacteria growth multidrug resistance pseudomonas aerginosa isolated from burns patients

Jasim,

Hussein

2023

Immune Response, Gene sequence to Contagious Ecthyma Virus (Orfv) Infected Lambs in Diyala Governorate, Iraq

Yuness,

Abood

2023

Contagious ecyhyma Known (Orf, contagious pustular dermatitis, sore mouth, scabby mouth) a disease spreads easily and brought by the epitheliotropic. It’s endemic around the world and has a serious economic impact. This study aimed to understand the immune response mechanisms of the lambs to defense against Contagious ecthyma virus and molecular detection of the virus with the determining the sequence and mutant of the viral genome to 91 lambs (41 has clinical signs of Orfv and 50 control do not have clinical sign of Orfv) for the period extended from September 2022 to April 2023. Blood samples were collected from all above groups to apply for detection the level of IgM, IgG, IL-10, and IFN-γ of Orfv by ELISA test. Positive Orfv IgM was 75.6% infected lamb and 72% none infected, and positive Orfv IgG was 90.2% infected lamb and 88% none infected. The result was showed the level of IL-10 a significant increasing, in all Lambs positive IgM and IgG compared with control group. IFN-γ level showed non significant differences between all Lambs positive IgM and IgG compared with control group. Molecular detection of Orfv result was confirmed the positivity of all samples positive Orfv IgM. And the sequencing of the Orfv DNA showed a mutant in the some Allel compared with References strain registered at NCBI. Concluded: high prevalence of Orfv among lambs and happened mutants to the viral genome increased the virus’s virulence by affecting the immune response.

Evaluation of the BioFire FilmArray Pneumonia Panel Plus for Detection of Viral, Bacterial Pathogens and Antimicrobial Resistance Genes in Lower Respiratory Tract Specimens at Al-Zahra Hospital for Pediatrics in Najaf, Iraq

Qasim Salih Mahdi,

Salim Saaid Tuwaij,

Abdul Hussein Mejbel

2024

The study aimed to evaluate the BioFire FilmArray Pneumonia Panel (BFPP) in rapidly diagnosing pathogens and detecting antimicrobial resistance genes in pediatric respiratory specimens, with an aim to enhance clinical decision-making in a pediatric hospital in Najaf, Iraq. For a period from 24/11/2022 to 22/5/2023, 36 specimens from pediatric patients with lower respiratory tract infection of males and females of different ages less than six years were collected. All specimens met the inclusion criteria, where Procalcitonin (PCT) levels above 0.5 ng/ml and negative blood cultures. The most prevalent pathogen detected were Acinetobacter calcoaceticus-baumannii complex in 63.89% of samples. Staphylococcus aureus and Klebsiella pneumoniae were detected in 44.44% and 41.67% of specimens, respectively. Less frequent were Streptococcus pneumoniae and Escherichia coli at 19.44%, Pseudomonas aeruginosa at 16.67%, Haemophilus influenzae at 11.11%, and Moraxella catarrhalis and Serratia marcescens each at 5.56%. Adenovirus was the most frequently detected virus, in 33.33% of the total samples. Other viruses detected include Human Rhinovirus/Enterovirus 19.44%, Respiratory Syncytial Virus 16.66%, Parainfluenza 11.11%, Human Metapneumovirus and Influenza B account for 5.55% while Corona virus not detected at all specimens. VIM gene was found in 63.89% of specimens, underscoring extensive carbapenem resistance, while other resistance genes appeared sporadically like mecA/C in 13.88%, IMP in 5.55% and KPC in 2.77 while OXA-48 like not detected at all.