Bacteriological profile and antibiogram of blood culture isolates done by automated culture and sensitivity method in a neonatal intensive care unit in a tertiary care hospital in Odisha, India

Sarangi, Kausik Kumar; Pattnaik, Dipti; Mishra, Surya Narayan; Nayak, M. M.; Jena, J.

doi:10.18203/2349-3933.ijam20151015

Cited by 20 publications

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“…6 In present study, no statistical correlation was found when blood culture positivity was compared with variables like gender, birth weight, gestational age and mode of delivery using chi square test for statistical analysis. These results were consistent with the study conducted by Sarangi K et al, Maharaja P et al, Satyamurthi B et al [7][8][9] Out of 100 cases with suspected sepsis, culture positivity rate was 40% predominantly due to gram negative organisms. The most common organism isolated were Pseudomonas, Klebsiella, MRSA.…”

Section: Discussionsupporting

confidence: 92%

Clinical profile and outcome of early onset sepsis in high risk very low birth weight neonates

Shukla

Rawat

2018

Int J Contemp Pediatr

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Background: Neonatal sepsis is one of the main causes of mortality and morbidity, especially in very low birth weight neonates (birth weight <1499 grams) despite the progress in hygiene, introduction of new and potent antimicrobial agents for treatment and advanced measures for diagnosis. The aim of the study was to find correlation of clinical features and risk factors of neonatal sepsis in culture positive cases.Methods: A cross- sectional study was carried out in one hundred neonates with risk factors of septicemia after obtaining informed consent. Blood culture was done using Bactec Peds Plus/F Culture as a gold standard to diagnose septicaemia. Correlation of risk factors, clinical features with laboratory findings was obtained by using chi-square test. p-value of less than 0.05 was considered as significant.Results: Out of 100 neonates with suspected sepsis, BACTEC culture proven sepsis was seen in 40% cases. Gram negative sepsis was seen in 62.5% cases. The most common bacteria for early onset sepsis were Klebsiella, Pseudomonas and MRSA contributing 17% each to the bacteriological profile. The most common predisposing factor and clinical feature in culture positive cases were Premature rupture of membrane >24 hours (67%) and bleeding/petechia/pupura (72%) respectively. The major cause of mortality was pulmonary hemorrhage.Conclusions: Gram negative organism were more common and associated with higher mortality. Blood culture positivity increases with increase in number of risk factors in neonatal septicemia. A detailed history and thorough clinical examination is vital for early recognition of sepsis.

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Section: Discussionsupporting

confidence: 92%

Clinical profile and outcome of early onset sepsis in high risk very low birth weight neonates

Shukla

Rawat

2018

Int J Contemp Pediatr

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“…In 2 Indian studies, K. pneumoniae was the most frequently isolated pathogen, followed by S. aureus and E. coli . [ 26 27 ] In contrast, with Garcia et al .,[ 25 ] Staphylococcus accounted for almost 2/3 of the organisms, followed by Enterococcus , Streptococcus agalactiae , and E. coli .…”

Section: Discussionmentioning

confidence: 97%

Time to positivity and antibiotic sensitivity of neonatal blood cultures

Abdelhamid

2017

J Global Infect Dis

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Background:Sepsis is a commonly encountered and potentially life-threatening problem in neonatal intensive care units, blood culture of neonatal sepsis helps in either optimizing treatment or terminating antibiotics.Materials and Methods:We determined the causative agent, time to positivity (TTP), and antibiogram of neonatal blood cultures collected in a tertiary care center, to investigate difference between early- and late-onset neonatal sepsis and to establish the time at which a blood culture could safely be considered negative, using the BacT/ALERT® 3D 60. A total of 826 clinically suspected neonates suffering from sepsis and admitted to a neonatal intensive care unit of a tertiary care hospital, Alexandria, Egypt were included in this study.Results:Eighty-five (10.29%) showed positive results. The overall TTP median was 21.1 h. Out of the 85 positive cultures, 57 (67.06%) were Gram-positive, 15 (17.65%) were Gram-negative, and 13 (15.29%) were fungi (all Candida). Coagulase-negative staphylococci were the predominant organism (41.18%). All the Gram-positive pathogenic isolates were sensitive to vancomycin and tigecycline. Among the Gram-negative isolates, maximum antibiotic sensitivity was observed for levofloxacin.Conclusion:We conclude that more than 3 days of incubation may not be required when using the BacT/ALERT® 3D 60 system.

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“…Sarangi et.al and Singh et.al. were also found that vancomycin and linezolid had highest antibiotic susceptibility NICU setting. 33,34 Highest prevalence isolates with multiple drug resistance that observed in our study may cause our hospital is a tertiary care center with large range health service not only in east java but also in east region Indonesia, Patient adjoining provinces are admitted for treatment that before attending the hospital, most of the patient get different antibiotic from low level heath care centers or due to over the counter sell of antibiotics often in improper dose. Limited population in some specimen and obtain of some pathogen or contaminant bacteria were all limitation in our study, multicenter prospective studies are needed to validated our finding.…”

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confidence: 78%

Microbial Pattern and Antibiotic Susceptibility in Pediatric Intensive Care Unit Dr. Soetomo Hospital, Surabaya

A.A.W.

Irwanto

Dharmawati

et al. 2019

IJTID

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Gram-negative bacterial are known as common pathogen caused infection in Pediatric Intensive Care Unit (PICU). Microbial Pattern and Antibiotic Susceptibility are needed as clinical data for selected appropriate antibiotic therapy. In PICU Dr. Soetomo hospital until now still lacking of Microbial Pattern and Antibiotic Susceptibility data. This descriptive study is to recognized Microbial Pattern and Antibiotic Susceptibility in PICU patients from blood, urine, sputum, stool, cerebrospinal fluid, endotracheal tube, pus swab and pleural fluid culture specimens. Patients whose admitted into PICU without signs of infections were excluded from the study. The inclusion criteria are patients with sign infection as follows: fever < 36,5°C or > 37.5°C, leukocyte < 4000/mm 3 or > 10000/mm 3 , marker infections CRP >10 mg/L or PCT >0,3 ng/mL, bradycardia or tachycardia, tachypnea, infiltrates on chest X-ray, turbid urine, dysuria, thrombophlebitis, abdominal pain or tenderness, and mucous or skin lesion. Medical record data from 2011 to 2016, revealed 1138 patients had positive microbial culture result, wherein positive result came from blood 44.46%, urine 19.15%, sputum 11.59%, stool 8.96%, cerebrospinal fluid 7.50%, endotracheal tube 4.04%, pus swab 2.89%, and pleural fluid 1.41%. The microorganisms found in PICU Dr. Soetomo was dominated with gram negative bacteria. Commonest bacterial that recognized from blood was B. cepacea, urine was E. coli, sputum was P. aeruginosa, Stool was E. coli, Cerebrospinal fluid was S. cohnii, endotracheal tube was K. pneumoniae ESBL, pus swab was S. aureus, and pleural fluid was S. maltophilia. Both gram-negative bacteria and gram-positive bacteria isolates revealed multiple drug resistance to commonly used antibiotic, but still had good susceptibility for antibiotic such as; amikacin, cefoperazone-sulbactam, linezolid, vancomycin and carbapenem group. ABSTRAKBakteri gram negatif merupakan patogen tersering penyebab infeksi di ruang rawat intensif anak. Pola bakteri dan kepekaan antibiotik diperlukan sebagai data klinis dalam pemilihan terapi antibiotik yang sesuai. Di ruang rawat intensif anak RS.Dr. Soetomo hingga saat ini masih sangat kekurangan data mengenai pola bakteri dan kepekaan antibiotik. Penelitian deskriptif ini bertujuan untuk membuat pola bakteri dan kepekaan antibiotika pada pasien yang dirawat di ruang rawat intensif anak dari spesimen darah, urin, sputum, feces, cairan serebrospinal,tabung endotrakeal (ETT), pus luka dan cairan pleura. Pasien yang masuk ke PICU yang tidak menunjukkan tanda dan gejala infeksi di eklusi dari penelitian. Kriteria inklusi pada penelitian ini adalah ditemukannya tanda dan gejala infeksi, antara lain ;demam < 36,5°C or > 37.5°C, kadar leukosit darah < 4000/mm3 or > 10000/mm3, marker infeksi CRP >10 mg/L or PCT >0,3 ng/mL, bradikardi atau takikardi, takipneu, gambaran infiltrate pada radiologi paru,urine yang keruh, nyeri berkemih, tromboplebitis, nyeri perut, dan lesi pada mukosa atau kulit. Dari data rekam medis dari tahun 2011 sampai...

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Bacteriological profile and antibiogram of blood culture isolates done by automated culture and sensitivity method in a neonatal intensive care unit in a tertiary care hospital in Odisha, India

Cited by 20 publications

References 0 publications

Clinical profile and outcome of early onset sepsis in high risk very low birth weight neonates

Clinical profile and outcome of early onset sepsis in high risk very low birth weight neonates

Time to positivity and antibiotic sensitivity of neonatal blood cultures

Microbial Pattern and Antibiotic Susceptibility in Pediatric Intensive Care Unit Dr. Soetomo Hospital, Surabaya

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