Bacteriological profile and antibiogram of neonatal septicemia in a tertiary care hospital

Mythri, Bikkasani; Patil, Ajinkya; Divya, A.; Mansabdar, Pooja; Sharon, V. A.

doi:10.5958/2394-5478.2016.00032.7

Cited by 3 publications

(6 citation statements)

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“…The E. coli isolates of this study showed resistance to AMP, CTX, CAZ, CIP and LEV and sensitivity towards SCF, CO, MEM, TGC, AK, FOS and TZP, in agreement with the literature [ 16 ]. The prevalence rate of P. aeruginosa in the current study is 17.6%, 23.1% of this was in female patients and 76.9% in male patients, as supported by the reported study [ 17 ]. A 2016 study conducted in Pakistan found P. aeruginosa in 13% of septicemia patients, 55.8% males and 44.2% females.…”

Section: Discussionsupporting

confidence: 87%

“…Determining the common pathogens and the antimicrobial susceptibility pattern causing septicemia is essential in order to select appropriate antibiotic therapies to decrease mortality and morbidity [ 17 ]. Keeping in view the importance of Gram-negative bacteria as one of the leading causes of septicemia, the current study was designed with the aim to determine the antibiotic susceptibility pattern, the molecular basis for antibiotic resistance and the mutations in selected genes of the bacterial isolates in Peshawar, Khyber-Pakhtunkhwa, Pakistan.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization and Epidemiology of Antibiotic Resistance Genes of β-Lactamase Producing Bacterial Pathogens Causing Septicemia from Tertiary Care Hospitals

et al. 2023

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Septicemia is a systematic inflammatory response and can be a consequence of abdominal, urinary tract and lung infections. Keeping in view the importance of Gram-negative bacteria as one of the leading causes of septicemia, the current study was designed with the aim to determine the antibiotic susceptibility pattern, the molecular basis for antibiotic resistance and the mutations in selected genes of bacterial isolates. In this study, clinical samples (n = 3389) were collected from potentially infected male (n = 1898) and female (n = 1491) patients. A total of 443 (13.07%) patients were found to be positive for bacterial growth, of whom 181 (40.8%) were Gram-positive and 262 (59.1%) were Gram-negative. The infected patients included 238 males, who made up 12.5% of the total number tested, and 205 females, who made up 13.7%. The identification of bacterial isolates revealed that 184 patients (41.5%) were infected with Escherichia coli and 78 (17.6%) with Pseudomonas aeruginosa. The clinical isolates were identified using Gram staining biochemical tests and were confirmed using polymerase chain reaction (PCR), with specific primers for E. coli (USP) and P. aeruginosa (oprL). Most of the isolates were resistant to aztreonam (ATM), cefotaxime (CTX), ampicillin (AMP) and trimethoprim/sulfamethoxazole (SXT), and were sensitive to tigecycline (TGC), meropenem (MEM) and imipenem (IPM), as revealed by high minimum inhibitory concentration (MIC) values. Among the antibiotic-resistant bacteria, 126 (28.4%) samples were positive for ESBL, 105 (23.7%) for AmpC β-lactamases and 45 (10.1%) for MBL. The sequencing and mutational analysis of antibiotic resistance genes revealed mutations in TEM, SHV and AAC genes. We conclude that antibiotic resistance is increasing; this requires the attention of health authorities and clinicians for proper management of the disease burden.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Epidemiology of Antibiotic Resistance Genes of β-Lactamase Producing Bacterial Pathogens Causing Septicemia from Tertiary Care Hospitals

et al. 2023

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“…The prevalence of gram-negative and gram-positive bacteria during the last seven years (that is, from 2011 to 2017) had a biphasic crossover trend, as reported by the studies in Turkey, Iran, and India (2,4,(11)(12)(13)(14). While gram-positives showed a growing trend, gram-negatives have been regressing until 2015, where they started reversing, and by 2018 gram-negatives reached 66%, and gram-positives dropped to 34% of all germs responsible for infantile sepsis (Figure 4).…”

Section: Discussionmentioning

confidence: 66%

“…Neonatal sepsis is a clinical syndrome characterized by the signs and symptoms of infection associated with positive blood culture (1,4). On-time diagnosis and identification of pathogens are important (5).…”

Section: Introductionmentioning

confidence: 99%

Sensitivity and Resistance of the Microorganisms Responsible for Neonatal Sepsis to Antibiotics

Boskabadi

Moradi

Ramazani

et al. 2020

Arch Clin Infect Dis

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Background: Sepsis is a major neonatal disease that requires appropriate early treatment. Objectives: The current study aimed to determine the resistance and sensitivity to antibiotics in neonates admitted to the neonatal intensive care unit (NICU) due to sepsis. Methods: In this cross-sectional study, 268 neonates with definitive sepsis (positive blood culture with clinical signs of infection) hospitalized during 2009-2019 are investigated. Identification of microorganism and antibiogram test were performed according to the standard microbiological method. Antibiotics susceptibility testing was performed by disk diffusion and microdilution method according to Clinical and Laboratory Standards Institute (CLSI) (2013). A researcher-made questionnaire, including characteristics of neonates and types of microorganisms (gram-positive or negative) in the neonatal ward, as well as sensitivity and resistance to common microorganisms in neonatal sepsis, was used to collect data. Results: According to the antibiogram results, the microorganism found in NICU showed sensitivity to vancomycin (97%), imipenem (71%), and co-trimoxazole (56%), as well as Norfloxacin, Cephalotin, and Cefazolin (50%). Gram-negative bacteria showed complete sensitivity (100%) to Piperacillin, Clavulanic acid, Colistin, Tazobactam, and Meropenem and high sensitivity (> 86%) to Imipenem, Norfloxacin, and Ciprofloxacin. There was high resistance to Amoxicillin, Ampicillin, Amikacin, Gentamicin, Cephalotin, Cefotaxime, Ceftriaxone, Ceftizoxime, Cephalexin, and Clindamycin. Gram-positive bacteria showed full sensitivity (100%) to Doxycycline, Piperacillin, and Tobramycin and reasonable sensitivity (> 75%) to Vancomycin, imipenem, and tetracycline. Azithromycin, Ampicillin, Gentamicin, Cefepime, Meropenem, Penicillin, Erythromycin, Oxacillin, and Amoxicillin showed resistance in 100% of cases. Conclusions: This study demonstrated high-sensitivity drugs for definitive treatment of neonatal sepsis (i.e., Vancomycin, Piperacillin, Tazobactam, and Meropenem for gram-negatives, and Piperacillin, and Tobramycin for gram-positive microorganisms).

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“…The importance of this classification. It helps to guide antibiotic therapy by implying differences in the mode of transmission and the predominant causative organisms [2]. EOS is caused mainly by bacteria transmitted from mothers to neonates during the intrapartum period, these are bacteria prevalent either in the maternal genital tract or in the area of delivery.…”

Section: Introductionmentioning

confidence: 99%

Bacteriological Profile and Antibiogram Pattern in Isolates From Neonatal Sepsis in a Tertiary Care Hospital

DEVI,

MANJULA,

KAMALA

2024

Int J Curr Pharm Sci

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Objective: To determine the microbiological profile and their Antibiogram pattern in isolates from neonatal sepsis. Methods: This study was conducted from October 2022 to January 15th 2023. Total 663 blood samples received from NICU, which were processed in the department of microbiology Andhra Medical College, Visakhapatnam. Organisms were identified by conventional methods and an antibiotic susceptibility test was done by Kirby Bauer disc diffusion method as per CLSI guidelines. Results: Out of 663 samples 200 (30.16%) are culture positives, in which males are more affected than females, that is 103 (51.5%). Among the culture positives, the most predominant isolate was klebsiella species 106 (53%) followed by pseudomonas 28 (14%) Escherichia coli 28 (14%) Acinetobacter 24 (12%), MRSA 11 (0.05%), MSSA 3(0.015%). Regarding its antibiogram, klebsiella species and Escherichia coli were most sensitive to penicillins+beta lactamase inhibitors (83%), fluroquinolones (75%) and aminoglycosides (75%), respectively. Pseudomonas, Acinetobacter was sensitive to penicillins+betalactamase inhibitors (100%) and aminoglycosides (72%). Gram-positive organisms were mostly sensitive to vancomycin, linezolid, aminoglycosides (MRSA) and cefoxitin (MSSA). There was widespread resistance to third-generation cephalosporins (77.77%) among gram-negative organisms. Conclusion: Gram negative bacteria are the most common cause of neonatal sepsis and klebsiella species are the predominant pathogens. These gram negative bacteria were resistant to cephalosporins. Antibiogram helps as a guide to antimicrobial therapy and resistance to antibiotics, as it is the common problem that causes ineffectiveness of empirical treatment.

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Bacteriological profile and antibiogram of neonatal septicemia in a tertiary care hospital

Cited by 3 publications

References 0 publications

Molecular Characterization and Epidemiology of Antibiotic Resistance Genes of β-Lactamase Producing Bacterial Pathogens Causing Septicemia from Tertiary Care Hospitals

Molecular Characterization and Epidemiology of Antibiotic Resistance Genes of β-Lactamase Producing Bacterial Pathogens Causing Septicemia from Tertiary Care Hospitals

Sensitivity and Resistance of the Microorganisms Responsible for Neonatal Sepsis to Antibiotics

Bacteriological Profile and Antibiogram Pattern in Isolates From Neonatal Sepsis in a Tertiary Care Hospital

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