Bacteriological Profile and Antimicrobial Sensitivity Pattern in Sterile Body Fluids from a Tertiary Care Hospital

Sharma, Rajani; Anuradha, .; Duggal, Nandini; ini,

doi:10.21767/2576-1412.100001

Cited by 11 publications

(29 citation statements)

References 8 publications

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“…In our study the most effective antibiotic against Gram negative organisms was colistin followed by carbapenems, amikacin and gentamicin. These findings are in agreement with the findings of Tullu MS et al [24] and Sharma et al [19] which also showed 100% sensitivity to colistin. Also, in our study Acinetobacter was the most resistant pathogens to many antibiotics which was also seen in some other studies [19] [25].…”

Section: A N T I B I O T I C S E N S I T I V I T Y P a T T E R N ( P supporting

confidence: 93%

“…These findings are in agreement with the findings of Tullu MS et al [24] and Sharma et al [19] which also showed 100% sensitivity to colistin. Also, in our study Acinetobacter was the most resistant pathogens to many antibiotics which was also seen in some other studies [19] [25]. Acinetobacter is an important public health problem, especially in ICUs and may cause severe infections with a high mortality rate [26].…”

Section: A N T I B I O T I C S E N S I T I V I T Y P a T T E R N ( P supporting

confidence: 93%

“…This finding was similar to Deb A et al study [13] and Harshika et al study [18]. However, this is in contrast to the study conducted by Sharma et al [19] where Acinetobacter spp. was the main isolate.…”

Section: A N T I B I O T I C S E N S I T I V I T Y P a T T E R N ( P supporting

confidence: 89%

“…In case of synovial fluid, we found Staphylococcus aureus (40%) Pseudomonas sp (20%) and Enterobacter sp (20%) and Serratia marcescens (20%) as the only isolates whereas other studies conducted isolated S. aureus, Klebsiella spp., Pseudomonas species and Enterococcus spp [19].…”

Section: A N T I B I O T I C S E N S I T I V I T Y P a T T E R N ( P mentioning

confidence: 99%

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Aerobic Bacteriological Profile and Antimicrobial Sensitivity Pattern of Bacteria Isolated from Sterile Body Fluids: A Study from a Tertiary Care Hospital in North India

Rouf

Nazir

2019

MRJI

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Aims: This study aims to isolate and identify the aerobic bacterial pathogens of sterile body fluids and to determine their susceptibility to various antibacterial agents. Study Design: This study was a retrospective observational study conducted in a tertiary care hospital. Place and Duration of Study: This study was conducted in the Department of Microbiology, SMHS hospital, Srinagar. A total of 814 samples were analysed for bacteriological culture and antibiotic sensitivity over a period of one year, from April 2018 to March 2019. Methodology: Clinical specimens (pleural fluid, ascitic fluid, cerebrospinal fluid, Synovial fluid, pericardial fluid and bile) were processed for bacterial culture according to standard procedures and antimicrobial susceptibility test for isolated organisms was done using Kirby Bauer disc diffusion method and interpreted as per Clinical and Laboratory Standards Institute (CLSI) recommendations. Results: In 814 samples of various body fluids, 88 samples showed growth of organism with an isolation rate of 10.81%. growth was most commonly seen in CSF (34.09%) followed by Ascitic fluid (23.86%, Bile (20.45), Pleural fluid (15.90%) and Synovial fluid (5.68%). No growth was obtained from pericardial fluid. The most predominant isolates were E. coli (23.86%), Pseudomonas sp (15.90%), Acinetobacter (14.77%), Klebsiella sp (7.95%), Staphylococcus aureus (11.36%), CONS (12.5%) and Enterococcus sp (4.54%). E. coli and Klebsiella were sensitive to imipenem, meropenem. colistin, amikacin and gentamicin. Staph. aureus and CONS were mostly sensitive to vancomycin, linezolid, and teicoplanin. Pseudomonas was sensitive to imipenem, meropenem, colistin and piperacillin/tazobactam. Acinetobacter, E. coli and Klebsiella sp were the most resistant organisms. Conclusion: In our study significant numbers of multidrug resistant bacteria were isolated from body fluids which calls for regular monitoring of prevalent pathogenic organisms and their sensitivities to avoid indiscriminate use of unnecessary antibiotics and the development of antibiotic resistance.

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Section: A N T I B I O T I C S E N S I T I V I T Y P a T T E R N ( P supporting

confidence: 93%

Section: A N T I B I O T I C S E N S I T I V I T Y P a T T E R N ( P supporting

confidence: 93%

Section: A N T I B I O T I C S E N S I T I V I T Y P a T T E R N ( P supporting

confidence: 89%

Section: A N T I B I O T I C S E N S I T I V I T Y P a T T E R N ( P mentioning

confidence: 99%

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Aerobic Bacteriological Profile and Antimicrobial Sensitivity Pattern of Bacteria Isolated from Sterile Body Fluids: A Study from a Tertiary Care Hospital in North India

Rouf

Nazir

2019

MRJI

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“…Infections at sterile body sites should be diagnosed and treated immediately due to the risk of severe morbidity and resulting into mortality [17]. Broadrange 16S rRNA PCR performed with clinical specimens may identify bacteria that cannot be grown in culture [18].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of direct 16S rRNA PCR from clinical samples for bacterial detection in normally sterile body sites

Satilmis

Toprak

Ilgın

et al. 2019

J Infect Dev Ctries

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Introduction: In addition to antibiotic treatment, slow-growing and non-cultivable bacteria can lead to false-negative results for sterile body site infections. In this study, we investigated the efficacy of 16S rRNA polymerase chain reaction (PCR) for such infections. Methodology: Following routine culture procedures, 16S ribosomal RNA (16S rRNA) PCR was performed for samples collected from sterile body sites between July 2017and September 2018. The samples were separated into two groups for likely (group 1) and unlikely infections (group 2) based on clinical and laboratory findings, as well as clinician opinion. Sequence analysis was performed for PCR-positive samples using 16S rRNA primers. Mixed chromatograms were analyzed with the RipSeq Mixed program, and Stata 15.1 was used for statistical analysis. Results: Eighty-seven of 139 samples collected from 116 patients were placed in group 1, and 52 were placed in group 2. Compared with culture as the reference method, the sensitivity, specificity, positive predictive value, and negative predictive value for 16S rRNA PCR were 89.8%, 85.6%, 77.2%, and 93.9%, respectively. 16S rRNA PCR identified infections in 13 culture-negative samples. Among these, three had Bartonella quintana, Mycoplasma salivarium, and Mycobacterium avium complex infections, which cannot be detected with commercial multiplex PCR kits. Conclusions: Our study demonstrates that 16S rRNA PCR is effective for the diagnosis of sterile body site infections, especially for cases of meningitis and infective endocarditis where routine cultures fail.

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Gut microbiota and changes in cytokine profile in animals with experimental acute disseminated peritonitis on the background of diabetes

Verveha,

Gutyj,

Holubiev

et al. 2023

Regul. Mech. Biosyst.

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In the pathogenesis of acute widespread peritonitis and accompanying diabetes, a vital link is an endogenous intoxication caused by the translocation of microorganisms and their toxins from the intestine into the blood, metabolic changes, and immunological reactivity of the body. Our work aimed to investigate the microbial composition in the parietal intestinal biotope and the features of the blood cytokine profile in animals with acute disseminated peritonitis on the background of streptozotocin-induced diabetes. The study was conducted on 56 sexually mature non-linear white male rats. Diabetes mellitus was modeled by a single intraperitoneal injection of streptozotocin (60 mg/kg). On the 14th day of the development of streptozotocin-induced diabetes mellitus, a 10% filtered fecal suspension (0.5 mL) was injected into the abdominal cavity of animals, and acute generalized peritonitis was initiated. Sowing on nutrient media was carried out for bacteriological research to isolate a pure culture of microorganisms and their identification. The concentration of TNF-α, IL-1β, and IL-6 was studied by solid-phase enzyme immunoassay. The research results demonstrate an imbalance of cytokines in the dynamics of experimental acute disseminated peritonitis against the background of diabetes and quantitative and qualitative changes in the microbiota of the parietal intestinal biotope. A decrease in the number of Escherichia coli strains isolated in monoculture and an increase in the number of two-component and three-component microbial associations were revealed, among which Enterobacter aerogenes, Escherichia coli, Bacteroides spp., Proteus mirabilis, Klebsiella spp. and Candida species prevailed.

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Bacteriological Profile and Antimicrobial Sensitivity Pattern in Sterile Body Fluids from a Tertiary Care Hospital

Cited by 11 publications

References 8 publications

Aerobic Bacteriological Profile and Antimicrobial Sensitivity Pattern of Bacteria Isolated from Sterile Body Fluids: A Study from a Tertiary Care Hospital in North India

Aerobic Bacteriological Profile and Antimicrobial Sensitivity Pattern of Bacteria Isolated from Sterile Body Fluids: A Study from a Tertiary Care Hospital in North India

Evaluation of direct 16S rRNA PCR from clinical samples for bacterial detection in normally sterile body sites

Gut microbiota and changes in cytokine profile in animals with experimental acute disseminated peritonitis on the background of diabetes

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