Despite advances in infection control surgical site infections formerly called surgical wound infections, remain a substantial cause of morbidity and mortality among hospitalized patients. The post-operative surgical site infection is the third most commonly reported nosocomial infections, accounting for a quarter of all such infections. The early stages of invasive infection caused by Staphylococcus aureus, also play major role in this process. In this present study a total of 50 pus samples were collected from patients having postoperative wound infections from the different surgical departments in the PSG Institute of Medical Sciences and Reasearch (PSG IMSR), Coimbatore during Dec 2016 to July 2017. Identifi cation of bacterial isolates was done by standard microbiological techniques. Further, the antimicrobial susceptibilities were done against following antibiotics, Penicillin (10 units), Chloramphenicol (30mcg), Vancomycin (30mcg), Streptomycin (10mcg), Neomycin (30mcg), Ampicillin (10mcg), Amoxicillin (10mcg), Amikacin (10mcg), Gentamycin (10mcg) and Ciprofl oxacin (10mcg). Among these strains 100%, 30%, 90%, 50%, 40%, 100%, 50%, 20%, 30% and 60% were found to be exhibiting a signifi cant degree of resistance to antibiotics tested. Currently, the development of bacterial resistance has necessitated the search for new antibacterial agents to combat the infectious disease using bio-medically active different solvent extract of Tribulus terrestris for antimicrobial activity. In this present study the maximum inhibition was observed in Ethyl acetate, moderate inhibition in Ethanol, minimum inhibition in Acetone, Chloroform, Methanol and no inhibition in aqueous and Petroleum ether. The results confi rm that, the plant appears to contain substances that exhibit broad antimicrobial activity against wound pathogens.