Bacteriological Profile of Wound Infection and Antibiotic Susceptibility Pattern of the Isolates

Abstract

Introduction: Wound infection is the one of the most important causes of morbidity and mortality worldwide and antibiotics resistant bacteria are the great part of complications on treatment of the infection.Objectives: The present study was conducted to isolate and identify the etiological agents of wound infection and assess the antimicrobial susceptibility pattern of the isolates. Methods:The study was carried out on wound infection suspected patients visiting B and B Hospital for six months duration. The collected pus specimen were first observed macroscopically then streaked on MacConkey agar and blood agar, incubated at 37 0 C for 24 hours. The isolated bacteria were identified by macroscopic and microscopic observations and biochemical reactions. Antibiotic susceptibility pattern of the isolates was assessed by Modified Kirby Baur disc diffusion technique.Results: A total of 503 pus samples were collected; of which 43.7% showed bacterial growth. Out of a total 220 bacterial isolates; 158 (71.82%) were Gram negative and 62 (28.18%) were Gram positive bacteria. Pseudomonas spp. (34.55%) was most common followed by Staphylococcus aureus (21.36%), Escherichia coli (11.82%), Acinetobacter baumannii (11.36%), Enterobacter aerogens (8.18%), Coagulase negative Staphylococci (CoNS) (5.45%), Klebsiella pneumoniae (4.55%), Citrobacter freundii (1.36%), Streptococcus spp. (0.91%) and Enterococcus spp. (0.46%). Most of Gram negative bacteria (63.9%) and Gram positive bacteria (93.5%) were susceptible to amikacin and chloramphenicol respectively. Among 220 isolates, 138 (62.73%) were found to be multi drug resistant (MDR). All the isolates of A. baumannii were isolated from in-patients and almost 96.4% (24/25) were MDR. Conclusion:In this study, significant number of MDR strains was found as the causative agent of wound infection. So, routine microbiological analysis of the wound specimen and their antibiotic susceptibility testing are recommended that will guide medical practitioners for empirical treatment of wound infection, so as to reduce the spread of resistant bacteria.

Antibiotic Susceptibility Pattern of Staphylococcus aureus Isolated from Pus/Wound Swab from Children Attending International Friendship Children's Hospital

“…The result was seems to be related to the study conducted by Sultana et al (2015) 40.45% S. aureus followed by E. coli 28.18% [38]; Barakoti et al (2017) 41.45% S. aureus followed by E. coli 22.79% [39]; Bankar et al (2018) 34.21% S. aureus followed by E. coli 23.02% [40] and Shahi et al (2018)70.6% S. aureus [41]. However Mahat et al [42] and Patil et al [34] Pseudomonas spp. as predominant organism.…”

Bacterial profile of blood stream infections and their antibiograms

“…Microorganisms present in the circulating blood whether continuously or intermittently are threat to every organ in the body. 1 Approximately 200000 cases of bacteraemia & fungemia occur annually with mortality rates ranging from 20-50%. 2 The detection of microorganisms in a patient's blood has great diagnostic & prognostic significance.…”

Bacteriological profile and antibiotic susceptibility patterns of wound infections in a tertiary care hospital in South India

“…1 Exposure of the subcutaneous tissue following loss of skin integrity provides an environment that is moist, warm, and nutritive, all of which are conducive for microbial colonization and proliferation. 2 Wound injuries are the most common and serious types of trauma and represent a major public health concern. There are many potential factors (age, sex, diabetes, stress, nutrition, oxygenation) involved in the complex wound healing process that can delay healing.…”

“…7 Wound infections are typically polymicrobial, and harbor bacteria, fungi, parasites and viruses that can be aerobic/anaerobic/facultative in nature. 2 The most common causative agents include Staphylococcus aureus, accounting for 20-40% of the infection, methicillin-resistant S. aureus, followed by Pseudomonas aeruginosa (5-15%), Escherichia coli, Enterococcus sp., Proteus sp. and Klebsiella sp.…”