Bacteriological quality of commonly consumed fruit juices and vegetable salads sold in some fruit juice houses in Addis Ababa, Ethiopia

Abstract

This study was conducted by collecting samples of 63 fruit juices and 21 vegetable salads from 21 juice houses. The survey indicated poor kiosk sanitary situation and personal hygiene, and bad storage conditions. Mean average of total viable counts of the samples of juices and vegetable salads was found to be 5.96 log cfu/g. The highest and lowest mean total coliform counts (TCCs) were that of avocado (1.17 log cfu/mL) and mango (0.51 log cfu/mL) juice samples. Mean fecal coliform counts were between 0.04 and 0.09 log cfu/mL. Treatment with sodium benzoate (0.1%) was found effective in reducing the total viable count. Escherichia coli and Salmonella were detected from 32.14% and 3.6% of samples, respectively. All E. coli and Salmonella isolates were found resistant to vancomycin. Hygienic status of the kiosks, storage situation of prepared juices and vegetable salads, and ingredients are the critical contributing factors for high microbial load of juices and vegetable salads. Practical applications This study was conducted to assess the hygienic status, risk associated with consumption of juices and vegetable salads, and effectiveness of treatments used before serving in juice houses in Addis Ababa, Ethiopia. Our results showed the existing sanitary situation and bacteriological safety status of the preparation of juices and vegetable salads in the vending kiosks; moreover, the risks associated with the consumption of the products. The study has pointed out the need for targeted awareness creation training on the attitude and practices of workers in the houses. The findings may further serve as an additional evidence on the level of risk associated with the consumption of the products. The results of this study may also be used by the regulatory bodies to take the due care they need to exercise in licensing and during their regular inspection of the kiosks to reduce the degree of the problem to protect consumers.

Antibiotic Resistance in Microbes from Street Fruit Drinks and Hygiene Behavior of the Vendors in Delhi, India

“…About 2.5 billion people, mostly from the LMICs, consume street foods every day [1]. Contaminated fruit and vegetable juices have been reported to be associated with infectious disease outbreaks, producing high morbidity and mortality across the world [2][3][4][5][6]. The World Health Organization (WHO) has reported more than 600 million cases of food-related diseases in the world, resulting in 420,000 deaths and 27,000,000 years of life lost (YLL) [7].…”

Microbial Quality Assessment and Efficacy of Low-Cost Disinfectants on Fresh Fruits and Vegetables Collected from Urban Areas of Dhaka, Bangladesh

“…The tubes with lauryl sulphate tryptose broth (LTB) were inoculated with (10 −1 , 10 −3 , 10 −6 ) diluted samples for 48 h at 35 • C for the TCC and TFC count. In each broth, Durham tubes were inserted to observe the gas formation [39]. After 24 h of incubation, each set of positive tubes that displayed positive acid and gas were counted and calculated using the standard MPN table.…”

“…After 24 h of incubation, each set of positive tubes that displayed positive acid and gas were counted and calculated using the standard MPN table. The positive sample for the completed test was suspended in BGLBB medium at 1 mL and kept at 37 • C at 24-48 h, and the positive results for the tube were determined with the MPN table [39].…”

Accelerating progress in improving diets and nutrition in Ethiopia

“…Food safety is another parameter of diet quality that is often overlooked. Nutrient-dense foods, such as fruit, vegetables, dairy, and meat, have in many instances in Ethiopia been found to Sodium intake (g/day) commonly contain high concentrations of microbial pathogens (Amenu et al 2016;Kechero et al 2019;Molla et al 2004). A recent review of evidence from Ethiopia also suggested high food safety risks related to mycotoxins (Ayelign & De Saeger 2020).…”