Bacteriological studies with ampicillin/sulbactam on selected strains of gram-negative bacteria

Bello, Helia; Solar, E del; González, Carlos; García, Antonio Manuel Rodríguez; Urrea, Rodrigo; Zemelman, R

doi:10.1016/0732-8893(88)90079-x

Cited by 2 publications

(1 citation statement)

References 10 publications

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“…Due to their broad spectrum of clinical effectiveness and tolerability β-lactam antibiotics constitute one of the most important families of antimicrobial agents due [1][2][3]. Clinical efficacy of β-lactamase restricts the efficiency of β-lactam antibiotics and their therapeutic activity [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Quick bioanalytical method of quantification and validation of sulbactam in plasma using high performance liquid chromatography

Abu‐Shandi

2009

Open Chemistry

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Abstract:A quick method of quantitative determination of sulbactam in human plasma, using liquid chromatography-UV spectroscopy, has been developed and validated. After derivatization with imidazole, plasma samples were treated by direct deproteinization with acetonitrile as an extraction solvent. After ultracentrifugation, sulbactam extract was directly injected onto the LC column.Chromatographic separation was performed on TSK Gel Super ODS (50 mm × 4.6 mm i.d., 2 µm) using methanol and phosphate buffer with tetrabutylammonium hydroxide solution as a mobile phase. Gradient elution was employed. The method was fully validated according to the United States Food and Drug Administration requirements (linearity, precision, trueness, quantification limit, detection limit, recovery, specificity and stability). The calibration curves were linear within the concentration range of 0.05-4.0 μg mL -1. Good method/system precision and accuracy of the method were demonstrated. Warsaw and Springer-Verlag Berlin Heidelberg. © Versita

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Section: Introductionmentioning

confidence: 99%

Quick bioanalytical method of quantification and validation of sulbactam in plasma using high performance liquid chromatography

Abu‐Shandi

2009

Open Chemistry

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Ampicillin‐Sulbactam and Ticarcillin‐Clavulanic Acid: A Comparison of Their In Vitro Activity and Review of Their Clinical Efficacy

Itokazu,

Danziger

1991

Pharmacotherapy

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Sulbactam (SB) and clavulanic acid (CA) are irreversible inhibitors of the β‐lactamases in the Richmond and Sykes classes II—VI. When combined with ampicillin and ticarcillin, SB and CA, respectively, extend the spectrum of activity of these penicillins to include some β‐lactamase‐producing aerobes (Enterobacteriaceae, Hemophilus influenzae, staphylococci) and anaerobes (Bacteroides fragilis group) which would otherwise be resistant. Neither effectively inhibits the class I β‐lactamases frequently produced by Pseudomonas aeruginosa, Enierobacter, and Serratia, in part explaining the resistance observed with these organisms. Clinically, both agents were as effective as the comparative therapies in all but two of the trials reviewed. Given the current data, the decision to add these agents to the formulary should be based on hospital resistance patterns and on the cost of these antimicrobials in comparison to conventional therapies.

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Bacteriological studies with ampicillin/sulbactam on selected strains of gram-negative bacteria

Cited by 2 publications

References 10 publications

Quick bioanalytical method of quantification and validation of sulbactam in plasma using high performance liquid chromatography

Quick bioanalytical method of quantification and validation of sulbactam in plasma using high performance liquid chromatography

Ampicillin‐Sulbactam and Ticarcillin‐Clavulanic Acid: A Comparison of Their In Vitro Activity and Review of Their Clinical Efficacy

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