Rock crab (Cancer irroratus) is abundant in the southern Gulf of St. Lawrence and along coastlines of the Atlantic region of Canada. The objective of this work was to prepare a pasteurization schedule for Atlantic Canadian Rock Crab meat (claw, leg and body). Samples of 175 g of meat plus 52 g of 2% w/w brine or 125 g meat plus 102 g brine were placed in an 8 oz polypropylene container with an aluminum pull‐top lid. The slowest heating spot for a conductively heating product was found to be 3.0 cm below the lid. Pasteurization was evaluated at 81C, 82C and 83C to a pasteurization lethality of 40 min at 85C (z = 8.89 C). The heat penetration parameters obtained were jh= 1.703, fh= 34.8 min, jc= 1.549 and fc= 51.0 min. The optimal process was found to be 83C for 130 min. The shear strength for unprocessed crab leg samples was, on average, 0.33 N/mm2 (±0.06) compared to 0.24 N/mm2 (+0.02) for processed samples. This represents 27.4% reduction in the shear stress that is largely attributed to the processing method. Thirty two free amino acids were determined including 9 essential amino acids, and their total amounts ranged between 24.8% and 30.0% of the sample, on dry basis. The total amounts of essential amino acids in the processed and unprocessed samples were, respectively, 15.3 and 17.8 mg/g sample. No significant losses in the amounts of essential amino acids was observed between processed and unprocessed samples, except for methionine, isoleucine and lysine, which were significantly reduced in the processed samples.