This study focuses on explaining the role of the periodic opening of the Bonnet Carré Spillway on the faecal coliform levels in Lake Pontchartrain. Water quality data were collected by the Lake Pontchartrain Basin Foundation to analyse the faecal coliform bacteria levels, turbidity, salinity, temperature, and dissolved oxygen in the lake water. The results show that the faecal coliform concentrations were lower during the spillway opening than the preceding and subsequent months. Statistical analysis shows the dilution effect, characterized by an effective reduction in faecal coliform concentrations due to increase in water turbidity, coupled with decrease in water salinity and dissolved oxygen because of spillway opening. It is also indicated that the combine effect of more than one factor produces a significantly greater reduction in the faecal coliform than each factor acting independently. Periodic spikes in faecal coliform concentrations were observed at sampling points located near the outlets of streams and drainage canals with high settlement densities. A positive correlation (r = 0.78467) was found between the average faecal coliform counts and average monthly precipitations, highlighting the role of precipitation run-offs.The results of this study indicate that the spillway opening contributed to a reduction in the faecal coliform concentration in the lake's surface water during the studied periods.