Bacteriology of abdominal wounds in elective open colon surgery: a prospective study of 100 surgical wounds

Abstract

This article describes a prospective bacteriological study designed to assess the value of bacterial sampling during elective open colon surgery. Swabs of the subcutaneous tissue were taken after closure of the abdominal fascia, but before closure of the skin, in 100 patients during elective colon surgery. Of the 100 swabs, 52 were sterile with no resulting wound infection, while ten of the remaining 48 contaminated swabs were associated with a wound infection caused by colonic pathogens and nosocomial microorganisms. Bacterial sampling seems to be a useful method for the prediction of wound infection following elective colon surgery.

Bacteriology of Wounds Infections in a Tertiary Care Hospital

“…The predominant organism identified in 105 isolates was Staphylococcus aureus even though the percentage of gramnegative isolates were high, which coincides with the previous studies and stating that though there was dominance of gramnegative isolates observed in this study, Staphylococcus aureus remained a predominant isolate 8,9,5,7,1,10,3,6 . This study contrasts with the study done by P.R.Fa-Si-Oen, et al 11 where Escherichia coli dominance was reported. In gram-negative isolates, Pseudomonas was identified as a predominant isolate followed by Escherichia coli, which coincides with the studies done by Sanjay Dhar, Rakesh Saraf et al 5 , and Gayathree Nail, Srinivas R et al 12 and with Ohalete.…”

An Incision Closure Bundle for Colorectal Surgery

“…The risk of contamination is heightened in colorectal procedures, in which a significant multispecies bacterial load is encountered. In one study, researchers reported that 48% of incisions made during 100 elective open colon procedures were contaminated during surgery (from endogenous and exogenous sources), and 21% of these contaminated incisions developed a clinically relevant infection …”

“…In one study, researchers reported that 48% of incisions made during 100 elective open colon procedures were contaminated during surgery (from endogenous and exogenous sources), and 21% of these contaminated incisions developed a clinically relevant infection. 21 In one study, researchers reported that 48% of incisions made during 100 elective open colon procedures were contaminated during surgery.…”

“…55,57 Historically, the use of antibiotic or antiseptic additives in irrigation fluids has been common in certain procedures (eg, joint arthroplasties). Use of a dedicated sterile incision closure instrument tray • Expert opinion • ACS SSI prevention guideline 1 • Peer-reviewed papers [2][3][4][5] Irrigation with 0.05% CHG following the manufacturer's IFU before closure • Peer-reviewed papers [6][7][8][9][10][11][12][13][14][15][16] Use of antibacterial triclosan-coated sutures • Peer-reviewed papers [17][18][19][20][21][22][23][24][25][26][27][28] Removal of the surgical drape after applying the dressing • Expert opinion • AST guideline 29 Application of topical skin adhesive (with or without mesh) over subcuticular or absorbable skin suture or antimicrobial dressing…”

Exploring Different Strategies for Efficient Delivery of Colorectal Cancer Therapy

“…The bacterial count has been estimated to be over 10 11 per gram in the colon which accounts for almost one-third of the dry weight of feces in man, compared with 10 4 per gram in the duodenum [ 55 , 56 ]. These vast colonic microfloras consist of mainly anaerobic bacteria that secrete biodegradable enzymes including glucoronidase, xylosidase, arabinosidase, galactosidase, nitroreductase, and azoreductase, which is only present in the colon.…”